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81.
The free energy of inserting a protein into a membrane is determined by considering the variation in the spectrum of thermal fluctuations in response to the presence of a rigid inclusion. Both numerically and through a simple analytical approximation, we find that the primary effect of fluctuations is to reduce the effective surface tension, hampering the insertion at low surface tension. Our results, which should also be relevant for membrane pores, suggest (in contrast to classical nucleation theory) that a finite surface tension is necessary to facilitate the opening of a pore. 相似文献
82.
Prof. Yuping P Zeng Prof. Oded Shenkar Prof. Sangcheol Song Prof. Seung-Hyun Lee 《Management International Review》2013,53(3):477-509
Purpose: This study examines the relationship between an MNE’s experience with foreign direct investments (FDI) and subsidiary mortality when considering learning limitations that an MNE are susceptible to under the influence of national cultural differences. Methods: We apply event history analysis on a sample of subsidiaries of Korean MNEs during 1990–2006. Results: We find that subsidiary mortality has a negative relationship with FDI experience from countries culturally similar to both the MNE’s home country and the focal host country; an inverse U-shaped relationship with both experience from countries culturally similar to the home country but different from the focal host country, and experience from countries culturally different from the home country but similar to the focal host country. Experience from countries culturally dissimilar to both the focal host country and the home country has a negative effect when a subsidiary is located in countries dissimilar to the home country, and an insignificant effect when a subsidiary is located in countries similar to the home country. Conclusions: Our findings show that prior experience has different effects depending on where it is accumulated and that MNEs are susceptible to limitations both when drawing inferences from their FDI experiences and when applying these experiences toward subsequent FDIs. 相似文献
83.
The dynamic out-of-plane response of unreinforced masonry walls is investigated. The study combines analytical, numerical, and experimental methodologies. The paper focuses on structural schemes that involve supporting at the base and the top and yield a unidirectional (one-way) flexural action. First, the modeling concepts for the nonlinear dynamic analysis are discussed and used as a basis for a finite element formulation. The element is based on a first-order shear deformation theory with large displacements, moderate rotations, small strains, material nonlinearity, and a Rayleigh type of viscoelastic damping. The nonlinearities due to cracking and the inelastic response under cyclic compression are introduced through the constitutive model for the mortar. The experimental part includes shake-table testing under different levels of out-of-plane excitation and compressive loading. The experimental results and the numerical model quantify a range of physical phenomena, including the dynamic arching and rocking effects, the coupling of the axial (in the height direction) and the out-of-plane responses, the role of axial loading, and the vulnerability of the masonry construction to dynamic loads. The comparison between the numerical results and the experimental results examines the capabilities of the model and gains insight into the nonlinear dynamics of the masonry wall. 相似文献
84.
Sarah C. Atkinson Steven M. Heaton Michelle D. Audsley Oded Kleifeld Natalie A. Borg 《International journal of molecular sciences》2021,22(16)
The cytoplasmic retinoic acid-inducible gene-I (RIG-I)-like receptors (RLRs) initiate interferon (IFN) production and antiviral gene expression in response to RNA virus infection. Consequently, RLR signalling is tightly regulated by both host and viral factors. Tripartite motif protein 25 (TRIM25) is an E3 ligase that ubiquitinates multiple substrates within the RLR signalling cascade, playing both ubiquitination-dependent and -independent roles in RIG-I-mediated IFN induction. However, additional regulatory roles are emerging. Here, we show a novel interaction between TRIM25 and another protein in the RLR pathway that is essential for type I IFN induction, DEAD-box helicase 3X (DDX3X). In vitro assays and knockdown studies reveal that TRIM25 ubiquitinates DDX3X at lysine 55 (K55) and that TRIM25 and DDX3X cooperatively enhance IFNB1 induction following RIG-I activation, but the latter is independent of TRIM25’s catalytic activity. Furthermore, we found that the influenza A virus non-structural protein 1 (NS1) disrupts the TRIM25:DDX3X interaction, abrogating both TRIM25-mediated ubiquitination of DDX3X and cooperative activation of the IFNB1 promoter. Thus, our results reveal a new interplay between two RLR-host proteins that cooperatively enhance IFN-β production. We also uncover a new and further mechanism by which influenza A virus NS1 suppresses host antiviral defence. 相似文献
85.
Oded Amir Martin P. Bendsøe Ole Sigmund 《International journal for numerical methods in engineering》2009,78(12):1474-1491
In the nested approach to structural optimization, most of the computational effort is invested in the solution of finite element analysis equations. In this study, the integration of an approximate reanalysis procedure into the framework of topology optimization of continuum structures is investigated. The nested optimization problem is reformulated to accommodate the use of an approximate displacement vector and the design sensitivities are derived accordingly. It is shown that relatively rough approximations are acceptable since the errors are taken into account in the sensitivity analysis. The implementation is tested on several small and medium scale problems, including 2‐D and 3‐D minimum compliance problems and 2‐D compliant force inverter problems. Accurate results are obtained and the savings in computation time are promising. Copyright © 2008 John Wiley & Sons, Ltd. 相似文献
86.
de Ruiter G Wijsboom YH Oded N van der Boom ME 《ACS applied materials & interfaces》2010,2(12):3578-3585
The ever-increasing flow of information requires new approaches for high-density data storage (HDDS). Here, we present a novel solution that incorporates the easily accessible polymer poly(3,4-ethylenedioxythiophene) (PEDOT) with multistate memory. The electrical addressable polymer is able to store up to five different memory states, which are stable up to 20 min. The observed memory states are generated by the optical output signature of the PEDOT deposited on indium tin oxide (ITO) coated glass, upon applying specific electrical inputs. Moreover, the demonstrated platforms can be represented by a general logic circuit, which allows the construction of multistate memory, such as flip-flops and flip-flap-flop logic circuits. 相似文献
87.
The linear elastic structural behavior of steel beams strengthened with externally bonded composite materials is experimentally and analytically investigated. The paper focuses on the full-field inter-layer relative displacements between the beam and the FRP layer. Such displacements result from the interaction between the adhesively bonded components and it is the integrated outcome of the interfacial conditions and the deformability of the adhesive. As such, it is commonly adopted as the state variable in simplified bond shear stress–slip representations. This aspect, as well as other aspects of the global and localized structural response, is analytically and experimentally quantified. The experiment includes a simply supported steel beam strengthened with a CFRP plate. A 3D image correlation technique with sequential measurements is used for the assessment of the full-field inter-layer displacements along the beam. The analysis adopts a high order modeling approach that accounts for the 2D stress and displacement fields through the depth of the adhesive and a 1D shear stress–slip approach using only a linear increasing branch. The comparison between the results provides validation of the analytical and experimental capabilities with emphasis on the inter-layer effects. One of the interesting findings which is discussed and explained in this paper is the fact that the slip values calculated with the shear stress–slip approach are notably different from the ones that can be measured experimentally and determined by the high order model. 相似文献
88.
The nonlinear behavior of masonry arches strengthened with externally bonded composite materials is investigated. A finite-element (FE) formulation that is specially tailored for the nonlinear analysis of the strengthened arch is developed. The FE formulation takes into account material nonlinearity of the masonry construction and high-order kinematic relations for the layered element. Implementation of the above concept in the FE framework reduces the general problem to a one-dimensional nonlinear formulation in polar coordinates with a closed-form representation of the elemental Jacobian matrix (tangent stiffness). A numerical study that examines the capabilities of the model and highlights various aspects of the nonlinear behavior of the strengthened masonry arch is presented. Emphasis is placed on the unique effects near irregular points and the nonlinear evolution of these effects through the loading process. A comparison with experimental results and a discussion of the correlating aspects and the ones that designate needs of further study are also presented. 相似文献
89.
W Ahmad V Brancolini MF ul Faiyaz H Lam S ul Haque M Haider A Maimon VM Aita J Owen D Brown DJ Zegarelli M Ahmad J Ott AM Christiano 《Canadian Metallurgical Quarterly》1998,62(4):987-991
Lumbosacral defects on 20 patients were covered with a perforator-based flap. Cutaneous perforators derived from the 9th and 10th intercostal arteries, the 4th lumbar artery, and multiple gluteal perforators that penetrate the gluteus maximus muscle were used as vascular pedicles. Minor complications occurred in five cases. Using this method, minimal morbidity of the donor site is expected because the gluteus maximus need not be sacrificed. Accordingly, perforator-based flaps are especially indicated for ambulatory patients, but for paraplegic patients as well. Even in the event of recurrence, another perforator-based or musculocutaneous flap can be elevated from the ipsilateral side because of the presence of multiple perforators in the lumbosacral and gluteal regions. 相似文献
90.
We present a simple and concise discretization of the covariant derivative vector Dirichlet energy for triangle meshes in 3D using Crouzeix-Raviart finite elements. The discretization is based on linear discontinuous Galerkin elements, and is simple to implement, without compromising on quality: there are two degrees of freedom for each mesh edge, and the sparse Dirichlet energy matrix can be constructed in a single pass over all triangles using a short formula that only depends on the edge lengths, reminiscent of the scalar cotangent Laplacian. Our vector Dirichlet energy discretization can be used in a variety of applications, such as the calculation of Killing fields, parallel transport of vectors, and smooth vector field design. Experiments suggest convergence and suitability for applications similar to other discretizations of the vector Dirichlet energy. 相似文献