Calibration and quantification of fast intracellular motion (FIM) in living cells using correlation analysis

Video rate confocal laser scanning microscopy at the highest spatial and temporal resolution of backscattered light (BSL) imaging allowed for regular observation of fast intracellular motion (FIM) first revealed in living neoplastic cells. However, the absence of an objective evaluation has hampered further study of the mechanisms and biological significance of FIM. Particularly, a quantification of apparent differences in velocities that would complement and improve the current demonstration of FIM by color coding using the combination of red-green-blue (RGB) images had been missing. Standard methods of tracking or pattern recognition could not be applied because of the fuzzy nature of images of FIM. A search for a suitable method led to correlation analysis. It was calibrated on Brownian motion and a known type of motion, such as cell marginal ruffling, compared with FIM. Results approved its explanatory potential. Therefore, several crucial incidences of FIM could be analyzed. Apart from an argument against viewing FIM as a manifestation of simple Brownian motion, the correlation analysis of FIM in the adjacent peripheries of a rat fibroblast and a K4 rat sarcoma cell confirmed the notion of higher and uneven distribution of velocity of FIM in a tumor cell so far shown in color-coded images only. This result and other yet unpublished observations indicate that the velocity and topology of FIM can also contribute to a biological distinction between neoplastic and normal cells. Regular application of the correlation analysis should further expand the study of FIM for its mechanisms and predictive value. Such an approach should be thoroughly examined for a contribution to the knowledge of cancer cells.     

The Sales Tax Puzzle – Has the law of demand been repealed?

TRA86 removed the deductibility of state and local sales taxes from U.S. personal income taxes. This effectively increased the price to state governments of sales tax revenue relative to income tax revenues. This implies that state reliance on income taxes relative to sales taxes should have increased after TRA86 was implemented. Leading public finance economists investigated this in the period shortly following the reform and invariably found that the predicted substitution of income for sales taxes did not take place. In fact, several studies noted that state dependence on the sales tax increased relative to income taxes – hence the Sales Tax Puzzle. Several experts tried to rationalize their non-findings. These rationalizations are unconvincing. This paper asserts that the puzzle may be resolved in two different ways. The first, seemingly trivial, is that the analysis must incorporate sufficient time so as to allow for relatively complex adjustments. Our contributions here are to anchor this obvious point with a theoretical model related to earlier work of the authors, and an explicit empirical examination of the lag structures of individual states reactions to the comprehensive tax reform. The second demonstrates that the analysis must take into account regional shifts that were taking place in the U.S. during this period. When such compositional shifts involving political and deductibility patterns are explicitly introduced into the model, the paradoxical findings are resolved both in the long and in the short run. Our contribution here is a demonstration that the use of detailed state-level data, unlike any of the earlier work in this area, allows for the inclusion in the analysis of inter-regional shifts in various parameters. An additional contribution is the explicit use and emphasis of the propensity to itemize as an independent variable. The use of this variable ties this area of analysis of post tax reform behavior into a hitherto untapped strand of public-finance literature. The variable is both theoretically interesting, and proves to have experienced compositional shift patterns which help resolve the sales-tax puzzle paradox.     

A Hybrid AlGaInAs–Silicon Evanescent Amplifier

We report a hybrid AlGaInAs-silicon evanescent amplifier incorporating a silicon waveguide with a III-V gain medium. The optical mode of the hybrid amplifier is mostly confined to the silicon waveguide and evanescently coupled to the AlGaInAs quantum-well (QW) region where optical gain is provided by electrical current injection. These two different material systems are bonded by low-temperature oxygen plasma assisted wafer bonding at 300 degC. The fabricated device shows 13 dB of maximum chip gain with 11 dBm of output saturation power. Evanescent coupling allows a lower active region confinement factor to provide a higher saturation output power than amplifiers with centered QWs, which is important for applications that require linear amplification     

A Study of the Properties of Compacts from Silicified Microcrystalline Celluloses

ABSTRACT

The paper deals with a study of tensile strength and disintegration time of compacts made from silicified microcrystalline celluloses, Prosolv SMCC 90, and Prosolv HD 90, in dependence on compression force, addition of two types of lubricants, and two active ingredients. The lubricants were magnesium stearate and sodium stearyl fumarate in a concentration of 0.5%, the active ingredients being ascorbic acid and acetylsalicylic acid in a concentration of 50%.

Prosolv SMCC 90 proved to be better compatible than Prosolv HD 90; the compacts were of higher strength, which was markedly increased with increasing compression force. Prosolv HD 90 was more sensitive to additions of lubricants, and a greater decrease in strength was recorded due to the influence of sodium stearyl fumarate. The effect of lubricants on the strength of compacts in the presence of active ingredients was not identical. The disintegration time of compacts from Prosolv HD 90 without as well as with lubricants was shorter than from Prosolv SMCC 90 and was increasing with increasing compression force. Disintegration time was increased with added lubricants, and it was markedly shortened by addition of active ingredients. Compacts containing ascorbic acid possessed a shorter disintegration time than those containing acetylsalicylic acid, and it was not markedly influenced by the presence of lubricants.     

Seismic retrofitting of nonductile beam-column sub-assemblage using FRP wrapping and steel plate jacketing

The paper discusses the aspects of repair and retrofitting technique adopted for a damaged reinforced concrete beam-column joint specimen under cyclic loading. A specimen designed based on Indian Standard specifications with consideration of seismic load but without adopting ductile detailing (NonDuctile) was investigated under reverse cyclic loading. Then, the damaged nonductile specimen was repaired with epoxy mortar and grouted using low viscous polymer, and retrofitted using fiber reinforced plastic (FRP) wrapping in beam and column components and steel plate jacketing in joint region. The experimental results showed that the retrofitted specimen not only regained its original strength and stiffness but also has overcome the deficiencies of nonductile detailing. The present study shows that a proper repair and adequate retrofitting technique can be used for strengthening and improvement of damaged regions in reinforced concrete structures.     

Electing a leader in a ring with link failures

Summary We investigate the message complexity of electing a leader in a ring of asynchronous processors. Our work deviates from the previous works on electing a leader in that we consider the effect of link failures. A link is said to fail if some message sent through it never reaches its destination. We distinguish the case where n is known from the case n unknown. Our main result is a O(n · log n) algorithm for electing a leader on a n-processor ring (the case n is known).     

Dynamic routing in reentrant flexible manufacturing systems

Consider a flexible manufacturing system (FMS), with several parallel production lines. Each line is statistically balanced. Due to process time and yield variations, some workstations may be temporarily starved of parts during the FMS operation, while others may have too many. The purpose of the dynamic routing algorithm described here is to achieve real-time load balancing in a stochastic processing environment and thus to increase the performance of the system in throughput, workload balance and reduced work-in-process queues. We formulate the problem and develop an optimal stationary policy (for two lines that have a material handling transport between them) based on the input buffer state of each station.     

Effect of noble metals in the decomposition of nitrous oxide over Fe-ferrierites

The decomposition of nitrous oxide was studied over Fe-ferrierite, Me-ferrierites and Fe/Me-ferrierites (Me: Pt, Rh and Ru). Flow as well as batch experiments were carried out and showed a synergy between Fe and Me ions. Ions of noble metals in Fe-ferrierite increased the catalytic activity in the sequence Pt < Rh ≅ Ru. Addition of NO substantially decreased the decomposition of N₂O over Rh/ferrierite and Ru/ferrierite, but not over bimetallic ferrierites. NO _x species created during the decomposition of nitrous oxide alone as well as with addition of NO, and employment of nitrous oxide labeled with ¹⁸O allowed us to assume a changing decomposition mechanism in the presence of Me ions in Fe-ferrierites.