Physical, chemical and palynological characterization of avocado (Persea americana Mill.) honey in Israel

Various methods were used to characterize avocado honey in Israel. Perseitol, a unique sugar present only in avocado honey, served as an indicator for the degree of purity of avocado honey. Low avocado pollen counts made the common melissopalynology method ineffective at predicting the contribution of avocado nectar to honey. It was also found that the levels of fructose, sucrose and glucose do not uniquely characterize avocado honey. Potassium content and eight other minerals were linearly highly correlated to perseitol content. Moreover, pH level, absorbance and degree of darkness were significantly correlated to perseitol content, and can therefore be used to characterize avocado honey.     

Toxicity of sea nettle (Chrysaora quinquecirrha) fishing tentacle nematocyst venom in cultured rat hepatocytes

Sea nettle (Chrysaora quinquecirrha) venom (CQV) is known to be toxic to the cardiac, respiratory, renal and hepatic systems in animal models. However, the mechanism of toxicity of CQV on hepatocytes is unknown. We utilized isolated rat hepatocytes in culture and measured percentage total lactate dehydrogenase (LDH) release after direct exposure to CQV. Toxicity of CQV in this system produced a linear multiple-dose response curve as well as a linear single-dose kinetics curve. Neither extracellular calcium concentration nor intracellular calcium chelation had a statistically significant effect on the toxicity of CQV in our hepatocyte model. From these results it appears that CQV does not form large membrane channels similar to complement, nor does calcium appear to play a major role in the mechanism of toxicity in hepatocytes. The isolated rat hepatocyye culture and measure of LDH release provided a relatively simple and reproducible model for examining toxicity of CQV.     

Secure reliable multicast protocols in a WAN

Summary. A secure reliable multicast protocol enables a process to send a message to a group of recipients such that all correct destinations receive the same message, despite the malicious efforts of fewer than a third of the total number of processes, including the sender. This has been shown to be a useful tool in building secure distributed services, albeit with a cost that typically grows linearly with the size of the system. For very large networks, for which this is prohibitive, we present two approaches for reducing the cost: First, we show a protocol whose cost is on the order of the number of tolerated failures. Secondly, we show how relaxing the consistency requirement to a probabilistic guarantee can reduce the associated cost, effectively to a constant. Received: August 1997 / Accepted: July 1999     

Using AVL trees for fault-tolerant group key management

In this paper we describe an efficient algorithm for the management of group keys for group communication systems. Our algorithm is based on the notion of key graphs, previously used for managing keys in large Internet-protocol multicast groups. The standard protocol requires a centralized key server that has knowledge of the full key graph. Our protocol does not delegate this role to any one process. Rather, members enlist in a collaborative effort to create the group key graph. The key graph contains n keys, of which each member learns log₂n of them. We show how to balance the key graph, a result that is applicable to the centralized protocol. We also show how to optimize our distributed protocol, and provide a performance study of its capabilities. Published online: 26 October 2001     

[18F]PRIMATX,a New Positron Emission Tomography Tracer for Imaging of Autotaxin in Lung Tissue and Tumor-Bearing Mice

Autotaxin (ATX) is a secreted enzyme with tissue levels associated with tissue injury, which increase during wound healing and chronic fibrotic diseases. We selected [¹⁸F](R,E)-3-(4-chloro-2-((5-methyl-2H-tetrazol-2-yl)methyl)phenyl)-1-(4-((5-(2-fluoroethoxy)pyridin-2-yl)methyl)-2-methylpiperazin-1-yl)prop-2-en-1-one ([¹⁸F]PRIMATX, [¹⁸F] 2 ), a tracer for positron emission tomography, to image ATX expression in vivo. It successfully differentiates expression levels in lung tissue samples from idiopathic pulmonary fibrosis patients, and allows the detection of ATX-expressing tumors in living mice, confirming its potential for development as a clinical imaging agent.     

Geometrically superresolved lensless imaging using a spatial light modulator

In this paper we introduce an imaging system based on a reflective phase-only spatial light modulator (SLM) in order to perform imaging with improved geometric resolution. By using the SLM, we combine the realization of two main abilities: a lens with a tunable focus and a phase function that, after proper free-space propagation, is projected as an amplitude distribution on top of the inspected object. The first ability is related to the realization of a lens function combined with a tunable prism that yields a microscanning of the inspected object. This by itself improves the spatial sampling density. The second ability is related to a projection of a phase function that is computed using an iterative beam-shaping Gerchberg-Saxton algorithm. After the free-space propagation from the SLM toward the inspected object, an amplitude pattern is generated on top of the object. This projected pattern and a set of low-resolution images with relative shift are interlaced and, after applying the proper regularization method, a geometrically superresolved image is reconstructed.     

Inhibition of recombinant human mitochondrial aldehyde dehydrogenase by two intermediate metabolites of disulfiram

Disulfiram is used in aversion therapy for alcoholism. S-Methyl-N,N-diethylthiocarbamate (MeDTC) sulfoxide, a potent inhibitor of the target enzyme mitochondrial aldehyde dehydrogenase (ALDH2), is thought to be the principal active metabolite of disulfiram in vivo. We examined the effects on recombinant human ALDH2 of two intermediate metabolites of disulfiram, S-methyl-N,N-diethyldithiocarbamate (MeDDC) sulfoxide and MeDDC sulfine. MeDDC sulfoxide was a potent inhibitor of ALDH2 with an IC50 of 2.2 +/- 0.5 microM (mean +/- SD, N = 4) after preincubation with enzyme for 30 min. MeDDC sulfine was a relatively weak inhibitor of ALDH2 under the same conditions with an IC50 value of 62 +/- 14 microM. The inhibition of ALDH2 by both compounds was irreversible and did not require the cofactor NAD. The latter finding demonstrates that inactivation of ALDH2 is independent of the dehydrogenase activity of the enzyme. GSH blocked almost completely the inhibition by 20 microM of MeDDC sulfoxide and greatly diminished the inhibition by 200 microM of MeDDC sulfine. Inactivation by MeDDC sulfoxide was time dependent. MeDTC sulfoxide was a more potent inhibitor of recombinant human ALDH2 (IC50 = 1.4 +/- 0.3 microM after preincubation for 15 min) than either of the intermediate metabolites, and its inhibition was unaffected by GSH. Our results suggest that these newer intermediate metabolites of disulfiram, especially the more potent MeDTC sulfoxide, have the potential to inhibit the target enzyme ALDH2 in patients receiving disulfiram. However, until the significance of the interactions of the inhibitors with GSH is more fully understood, the contribution of MeDDC sulfine and MeDDC sulfoxide to the pharmacological effects of disulfiram in vivo is uncertain.     

User attitudes towards news content personalization

Personalizing news content requires to choose the appropriate depth of personalization and to assess the extent to which readers’ explicit expressions of interest in general and specific news topics can be used as the basis for personalization. A preliminary survey examined 117 respondents’ attitudes towards news content personalization and their interest in various news topics and subtopics. The second survey examined 23 participants’ declared and actual interests. Participants preferred personalization based on general news topics. Declared interest in general news topics adequately predicted the actual interests in some topics, while in others users’ interests differed between general news topics and subtopics. The variance in interest in items also differed among topics. Thus, different personalization methods should be used for different topics. For some, such as ‘Sports’, users show either high interest or no interest at all. In the latter case most articles related to the topic should be removed, with the exception of items that refer to unique events that may raise general interest according to the expressed interest. In other topics, such as ‘Science & Technology’, most users are interested in important articles, even if they are not interested in the general news topic. Here, the filtering technique should identify the important articles and present them to all readers. The results can be used to develop effective and simple personalization mechanisms which can be applied to the personalization of news, as well as to other domains.     

Capsular management and refractive error in pediatric intraocular lenses

The effects of dietary protein types and methionine supplementation on phospholipid metabolism were investigated to clarify the mechanism of the hypocholesterolemic action of soybean protein in rats fed a cholesterol-free diet. The effect of switching from a casein diet to a soybean protein diet was also investigated. Rats were fed casein, soybean protein or soybean protein + methionine diet for 14 d. Compared with casein diet, feeding of soybean protein diet led to significantly higher proportions of linoleic acid and linoleic acid-containing molecular species, especially 16:0-18:2, in plasma and liver microsomal phosphatidylcholine (PC). In addition, significantly lower plasma cholesterol concentration, hepatic S-adenosylmethionine concentration and liver microsomal PC:phosphatidylethanolamine ratio resulted. These alterations caused by the soybean protein diet were significantly suppressed by supplementing methionine to the level of the casein diet (3.4 g/kg diet). The proportion of the sum of certain plasma PC molecular species, which contain 18:1 or 18:2 in the sn-2 position, increased in response to the switch from the casein diet to the soybean protein diet at a rate similar to the decrease in plasma cholesterol concentration; there was a significant correlation between the two variables (r = -0.992, P < 0.001). These results indicate that about 40% of the hypocholesterolemic action of soybean protein is due to the low methionine content of the protein and might be associated with alterations of the plasma phospholipid molecular species profile.     

General Geometric Good Continuation: From Taylor to Laplace via Level Sets

Good continuation is the Gestalt observation that parts often group in particular ways to form coherent wholes. Perceptual integration of edges, for example, involves orientation good continuation, a property which has been exploited computationally very extensively. But more general local-global relationships, such as for shading or color, have been elusive. While Taylor’s Theorem suggests certain modeling and smoothness criteria, the consideration of level set geometry indicates a different approach. Using such first principles we derive, for the first time, a generalization of good continuation to all those visual structures that can be abstracted as scalar functions over the image plane. Based on second order differential constraints that reflect good continuation, our analysis leads to a unique class of harmonic models and a cooperative algorithm for structure inference. Among the different applications of good continuation, here we apply these results to the denoising of shading and intensity distributions and demonstrate how our approach eliminates spurious measurements while preserving both singularities and regular structure, a property that facilitates higher level processes which depend so critically on both of these classes of visual structures.