The social costs of emotional suppression: A prospective study of the transition to college.

There is growing interest in understanding how emotion regulation affects adaptation. The present study examined expressive suppression (which involves inhibiting the overt expression of emotion) and how it affects a critical domain of adaptation, social functioning. This investigation focused on the transition to college, a time that presents a variety of emotional and social challenges. Analyses focused on 2 components of suppression: a stable component, representing individual differences expressed both before and after the transition, and a dynamic component, representing variance specific to the new college context. Both components of suppression predicted lower social support, less closeness to others, and lower social satisfaction. These findings were robustly corroborated across weekly experience reports, self-reports, and peer reports and are consistent with a theoretical framework that defines emotion regulation as a dynamic process shaped by both stable person factors and environmental demands. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Measurement of Helium Leakage Rates through Closures of Dangerous Goods Packagings for the Assessment of Potentially Explosive Mixtures in Freight Containers

The objective was to find out whether an explosive atmosphere can be created in a freight container by gaseous leakage flow of vapour‐air‐mixture through leaks in the closures of dangerous goods packagings filled with hazardous liquids. Because of high temperatures during intercontinental carriage, there is a gauge pressure in the free vapour phase inside the packagings which can cause a gaseous leakage flow. Two different methods were applied: Helium limit leakage rates for 23 quantitatively important hazardous liquids concerning their lower explosion limit (LEL) were calculated for a worst case transport scenario (Method 1). Helium leakage rates of five closure types of dangerous goods packagings with volumes of approximately 6 l were measured using the pressure technique by accumulation (Method 2). All types of closures of steel packagings were uncritical. The maximum measured leakage was 33% of the limit leakage rate. The leakage rates of screw closures of plastic jerricans can exceed the LEL if there are production‐related patterns such as non‐concentricity of the closures and flashes on the neck. Especially for plastic packagings it is important to minimize gaseous leakage flow, because an explosive atmosphere can also be reached by permeation of the individual filling substance or by a combination of both effects. For the assessment of potentially explosive mixtures in freight containers, both mass transfer mechanisms have to be taken into account. Copyright © 2015 John Wiley & Sons, Ltd.     

Transparent,Stimuli‐Responsive Films from Cellulose‐Based Organogel Nanoparticles

The use of bio‐based nanoscaled cellulose for the construction of novel functional materials has progressed rapidly over the past years. In comparison to most of studies starting with the hydrophilic nanoscaled cellulose, surface‐stearoylated cellulose nanoparticles (SS‐CNPs) are used in this report for the construction of multifunctional, responsive films. SS‐CNPs with an average size of 115 ± 0.5 nm are obtained after the surface‐modification of cellulose under heterogeneous conditions. Crystalline cellulose core is present within SS‐CNPs according to solid‐state ¹³C nuclear magnetic resonance (NMR) spectroscopy. SS‐CNPs show excellent dispersibility in nonpolar solvents and form temperature‐responsive organogels in tetrahydrofuran (THF) at low temperature or after long time storage at room temperature. Moreover, transparent and self‐standing films of SS‐CNPs from their THF‐suspension show solvent‐responsive surface wettability and responsive shape‐memory property. SS‐CNPs can also be used for the fabrication of nanocomposite films together with nonpolar compounds, such as (2‐stearoylaminoethyl) rhodamine B. Thus, these novel SS‐CNPs derived from sustainable cellulose fibers are promising candidates for the construction of novel functional materials.     

Regenerative design,socio-ecological systems and co-evolution

A key notion in regenerative design is the co-evolutionary, partnered relationship between socio-cultural and ecological systems, which requires an explicit engagement with the implications and consequences of future design decisions. However, despite the extensive literature in other disciplines regarding the co-evolution of socio-cultural and ecological systems, this approach has yet to receive serious scrutiny within the context of the built environment and within the emerging notions of regenerative development and design. Drawing on an interdisciplinary body of literature, a discussion is initiated on how socio-cultural and ecological systems and their co-evolution might connect to the concept of regenerative design. Following a critique of a relevant example highlighting the current practice of regenerative design, the new building for the Centre for Interactive Research on Sustainability at the University of British Columbia, Vancouver, potentially relevant aspects of a socio-ecological system and of evolution theories are examined for the built environment. Several observations are presented on how these may offer a stronger theoretical framing of regenerative design, particularly the shifts in design thinking: from buildings as artefacts to their dynamic role in adaptive processes over time; widening the boundary focus of a building from its site to the neighbourhood.

Une notion clé du design régénérateur est la relation coévolutionnaire de partenariat entre les systèmes socioculturels et écologiques, ce qui nécessite un engagement explicite à l'égard des implications et des conséquences des futures décisions en matière de design. Cependant, malgré la vaste littérature existant dans les autres disciplines concernant la coévolution des systèmes socioculturels et écologiques, cette approche doit encore faire l'objet d'un examen sérieux dans le contexte de l'environnement bâti et sous l'angle des notions émergentes de développement et de design régénérateurs. En s'appuyant sur un corpus interdisciplinaire de publications, une discussion est amorcée sur la manière dont les systèmes socioculturels et écologiques et leur coévolution pourraient être reliés au concept de design régénérateur. Après une critique portant sur un exemple pertinent mettant en évidence les pratiques actuelles du design régénérateur, le nouveau bâtiment du Centre de Recherche Interactive sur le Développement Durable de l'Université de Colombie Britannique, à Vancouver, sont examinés les aspects potentiellement pertinents d'un système socioécologique et des théories évolutionnistes, s'agissant de l'environnement bâti. Plusieurs observations sont présentées quant à la manière dont ceux-ci peuvent offrir un cadre théorique plus solide au design régénérateur, concernant en particulier les changements intervenant dans la façon de penser le design : des bâtiments envisagés en tant qu'artéfacts à leur rôle dynamique dans les processus adaptatifs au fil du temps, et un bâtiment dont les limites ne se focalisent plus sur son site, mais sont élargies à son quartier.

Mots clés: environnement bâti, coévolution, cadres conceptuels, évolution, design régénérateur, systèmes socioécologiques     

Gallium gradients in Cu(In,Ga)Se2 thin‐film solar cells

The gallium gradient in Cu(In,Ga)Se₂ (CIGS) layers, which forms during the two industrially relevant deposition routes, the sequential and co‐evaporation processes, plays a key role in the device performance of CIGS thin‐film modules. In this contribution, we present a comprehensive study on the formation, nature, and consequences of gallium gradients in CIGS solar cells. The formation of gallium gradients is analyzed in real time during a rapid selenization process by in situ X‐ray measurements. In addition, the gallium grading of a CIGS layer grown with an in‐line co‐evaporation process is analyzed by means of depth profiling with mass spectrometry. This gallium gradient of a real solar cell served as input data for device simulations. Depth‐dependent occurrence of lateral inhomogeneities on the µm scale in CIGS deposited by the co‐evaporation process was investigated by highly spatially resolved luminescence measurements on etched CIGS samples, which revealed a dependence of the optical bandgap, the quasi‐Fermi level splitting, transition levels, and the vertical gallium gradient. Transmission electron microscopy analyses of CIGS cross‐sections point to a difference in gallium content in the near surface region of neighboring grains. Migration barriers for a copper‐vacancy‐mediated indium and gallium diffusion in CuInSe₂ and CuGaSe₂ were calculated using density functional theory. The migration barrier for the In_Cu antisite in CuGaSe₂ is significantly lower compared with the Ga_Cu antisite in CuInSe₂, which is in accordance with the experimentally observed Ga gradients in CIGS layers grown by co‐evaporation and selenization processes. Copyright © 2014 John Wiley & Sons, Ltd.     

Characterization of GPVI- or GPVI-CD39-Coated Nanoparticles and Their Impact on In Vitro Thrombus Formation

Traditional antithrombotic agents commonly share a therapy-limiting side effect, as they increase the overall systemic bleeding risk. A novel approach for targeted antithrombotic therapy is nanoparticles. In other therapeutic fields, nanoparticles have enabled site-specific delivery with low levels of toxicity and side effects. Here, we paired nanotechnology with an established dimeric glycoprotein VI-Fc (GPVI-Fc) and a GPVI-CD39 fusion protein, thereby combining site-specific delivery and new antithrombotic drugs. Poly(lactic-co-glycolic acid) (PLGA) nanoparticles, NP-BSA, NP-GPVI and NP-GPVI-CD39 were characterized through electron microscopy, atomic force measurements and flow cytometry. Light transmission aggregometry enabled analysis of platelet aggregation. Thrombus formation was observed through flow chamber experiments. NP-GPVI and NP-GPVI-CD39 displayed a characteristic surface coating pattern. Fluorescence properties were identical amongst all samples. NP-GPVI and NP-GPVI-CD39 significantly impaired platelet aggregation. Thrombus formation was significantly impaired by NP-GPVI and was particularly impaired by NP-GPVI-CD39. The receptor-coated nanoparticles NP-GPVI and the bifunctional molecule NP-GPVI-CD39 demonstrated significant inhibition of in vitro thrombus formation. Consequently, the nanoparticle-mediated antithrombotic effect of GPVI-Fc, as well as GPVI-CD39, and an additive impact of CD39 was confirmed. In conclusion, NP-GPVI and NP-GPVI-CD39 may serve as a promising foundation for a novel therapeutic approach regarding targeted antithrombotic therapy.     

Loss of SDHB Induces a Metabolic Switch in the hPheo1 Cell Line toward Enhanced OXPHOS

Background: Enzymes of tricarboxylic acid (TCA) have recently been recognized as tumor suppressors. Mutations in the SDHB subunit of succinate dehydrogenase (SDH) cause pheochromocytomas and paragangliomas (PCCs/PGLs) and predispose patients to malignant disease with poor prognosis. Methods: Using the human pheochromocytoma cell line (hPheo1), we knocked down SDHB gene expression using CRISPR-cas9 technology. Results: Microarray gene expression analysis showed that >500 differentially expressed gene targets, about 54%, were upregulated in response to SDHB knock down. Notably, genes involved in glycolysis, hypoxia, cell proliferation, and cell differentiation were up regulated, whereas genes involved in oxidative phosphorylation (OXPHOS) were downregulated. In vitro studies show that hPheo1 proliferation is not affected negatively and the cells that survive by shifting their metabolism to the use of glutamine as an alternative energy source and promote OXPHOS activity. Knock down of SDHB expression results in a significant increase in GLUD1 expression in hPheo1 cells cultured as monolayer or as 3D culture. Analysis of TCGA data confirms the enhancement of GLUD1 in SDHB mutated/low expressed PCCs/PGLs. Conclusions: Our data suggest that the downregulation of SDHB in PCCs/PGLs results in increased GLUD1 expression and may represent a potential biomarker and therapeutic target in SDHB mutated tumors and SDHB loss of activity-dependent diseases.     

Prevalence, distribution, and diversity of Listeria monocytogenes in retail environments, focusing on small establishments and establishments with a history of failed inspections

Despite growing concerns about cross-contamination of ready-to-eat foods with Listeria monocytogenes, our knowledge about the ecology and transmission of L. monocytogenes in retail establishments has remained limited. We conducted a cross-sectional study to characterize the prevalence, distribution, and subtype diversity of L. monocytogenes in 120 New York State retail deli establishments that were hypothesized to present an increased risk for environmental L. monocytogenes contamination (i.e., small establishments and establishments with a history of failed New York State Agriculture and Markets inspections). Analysis of these data along with previously reported data for 121 predominantly larger retail establishments in New York State identified establishment size, geographic location, and inspection history as significant predictors of L. monocytogenes presence and prevalence. The odds of an establishment being L. monocytogenes positive were approximately twice as high for large establishments, establishments located in New York City, or establishments with poor inspection history (as compared with establishments without these attributes), even though correlation between location and inspection history complicated interpretation of results. Within an establishment, L. monocytogenes was significantly more prevalent on nonfood contact surfaces than on food contact surfaces; prevalence was particularly high for floors and in floor drains, sinks, the dairy case, and milk crates. L. monocytogenes subtype diversity differed between sites, with lineage I isolates significantly associated with nonfood contact surfaces and lineage II isolates significantly associated with food contact surfaces. Isolates belonging to the same ribotype were often found dispersed across multiple sites within an operation.