AGEs-Induced IL-6 Synthesis Precedes RAGE Up-Regulation in HEK 293 Cells: An Alternative Inflammatory Mechanism?

Advanced glycation end products (AGEs) can activate the inflammatory pathways involved in diabetic nephropathy. Understanding these molecular pathways could contribute to therapeutic strategies for diabetes complications. We evaluated the modulation of inflammatory and oxidative markers, as well as the protective mechanisms employed by human embryonic kidney cells (HEK 293) upon exposure to 200 μg/mL bovine serum albumine (BSA) or AGEs–BSA for 12, 24 and 48 h. The mRNA and protein expression levels of AGEs receptor (RAGE) and heat shock proteins (HSPs) 27, 60 and 70, the activity of antioxidant enzymes and the expression levels of eight cytokines were analysed. Cell damage via oxidative mechanisms was evaluated by glutathione and malondialdehyde levels. The data revealed two different time scale responses. First, the up-regulation of interleukin-6 (IL-6), HSP 27 and high catalase activity were detected as early as 12 h after exposure to AGEs–BSA, while the second response, after 24 h, consisted of NF-κB p65, RAGE, HSP 70 and inflammatory cytokine up-regulation, glutathione depletion, malondialdehyde increase and the activation of antioxidant enzymes. IL-6 might be important in the early ignition of inflammatory responses, while the cellular redox imbalance, RAGE activation and NF-κB p65 increased expression further enhance inflammatory signals in HEK 293 cells.     

Isolation and Characterization of the First Microsatellite Markers for the Endangered Relict Mussel Hypanis colorata (Mollusca: Bivalvia: Cardiidae)

Hypanis colorata (Eichwald, 1829) (Cardiidae: Lymnocardiinae) is a bivalve relict species with a Ponto-Caspian distribution and is under strict protection in Romania, according to national regulations. While the species is depressed in the western Black Sea lagoons from Romania and Ukraine, it is also a successful invader in the middle Dniepr and Volga regions. Establishing a conservation strategy for this species or studying its invasion process requires knowledge about the genetic structure of the species populations. We have isolated and characterized nine polymorphic microsatellite markers in H. colorata. The number of alleles per locus ranged from 4 to 28 and the observed heterozygosity ranged from 0.613 to 1.000. The microsatellites developed in the present study are highly polymorphic and they should be useful for the assessment of genetic variation within this species.     

Weak Tangency, Weak Invariance, and Carathéodory Mappings

New results on weak invariance in Hilbert spaces, both in autonomous and nonautonomous case, are given. Applications to weakly decreasing systems and to strong invariance are presented.     

Pose estimation for objects with planar surfaces using eigenimage and range data analysis

In this paper we present a novel method for estimating the object pose for 3D objects with well-defined planar surfaces. Specifically, we investigate the feasibility of estimating the object pose using an approach that combines the standard eigenspace analysis technique with range data analysis. In this sense, eigenspace analysis was employed to constrain one object rotation and reject surfaces that are not compatible with a model object. The remaining two object rotations are estimated by computing the normal to the surface from the range data. The proposed pose estimation scheme has been successfully applied to scenes defined by polyhedral objects and experimental results are reported.     

Volumetric hydrogen adsorption capacity of densified MIL-101 monoliths

The hydrogen adsorption isotherms of MIL-101 compressed pellets at 77.3 K are reported. The specific surface area and micropore volume decrease rather sharply when the pellet density approaches the crystal density. Optimum volumetric storage capacity of 40 g L⁻¹ is obtained for monoliths of remarkable mechanical integrity. The X-ray diffraction patterns do not exhibit notable changes with compression up to densities close to the crystal density. However, the infrared spectra show significant modification of the band structure in the range of vibration frequencies characteristic to the carboxylate and phenylene groups, due to the pressure-induced changes in the coordination environment of the metal, close to the adsorption sites. The compression effect on hydrogen adsorption can be correlated with the changes in the nitrogen adsorption isotherms. The results are discussed and compared with the literature results on volumetric hydrogen storage capacity of MOF-5 and MOF-177 monoliths.     

Magnetic and dielectric properties of Co–Zn ferrite

Nanocrystalline powders of Co substituted Zn ferrite with the chemical formula Co_xZn_1−xFe₂O₄ (x = 0, 0.2, 0.4, 0.6, 0.8, 1) were synthesized by sol–gel autocombustion method using tartaric acid as fuel agent. The samples were sintered in static air atmosphere for 7 h at 773 K, 7 h at 973 K and 10 h at 1173 K. The organic phase extinction and the spinel phase formation were monitored by means of Fourier transform infrared spectroscopy. The X-ray diffraction patterns analysis confirmed the spinel single phase accomplishment. Crystallite size, average grains size, lattice parameter and cation distribution were estimated. Magnetic behavior of the as-obtained samples by means of M-H hysteresis measurements was studied at room temperature. Permeability and dielectric permittivity at room temperature versus frequency was the subject of a comparative study for the Co_xZn_1−xFe₂O₄ series. In agreement with the proposed cation distribution the sample with Co_0.8Zn_0.2Fe₂O₄ formula exhibits the optimal magnetic and dielectric properties.     

3D morphology of Au and Au@Ag nanobipyramids

The morphologies of Au and Au@Ag nanobipyramids were investigated using electron tomography. The 3D reconstruction reveals that the Au bipyramids have an irregular six-fold twinning structure with highly stepped dominant {151} facets. These short steps/edges stabilized via surface adsorbed CTAB favor the growth of silver on the lateral facets leading to strong blue shifts in longitudinal plasmon surface resonance.