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991.
A deletion mutant of the catalytic RNA component of Escherichia coli RNase P missing residues 87-241 retains the ability to interact with the protein component to form a functional catalyst. The deletion of this phylogenetically conserved region significantly increases the Km, indicating that the deleted structures may be important for binding to the precursor tRNA substrate but not for the cleavage reaction. Under some reaction conditions, this RNase P deletion mutant can become a relatively non-specific nuclease, indicating that this RNA's catalytic center may be more exposed. The catalytic core of the RNase P is formed by less than one third of the 377 residues of the RNase P RNA. 相似文献
992.
P Fauran 《Canadian Metallurgical Quarterly》1996,89(2):163-4; discussion 165
Since the 1940s, the dengue epidemics occur more and more often in the Pacific islands with an increased severity. For example, in New Caledonia, outbreaks of dengue-like diseases have been reported since the end of the last century but the first epidemic due to an identified virus occurred in 1942-1943 and was caused by the DEN-1 type. The next, due to the DEN-2 type, was reported thirty years later, in 1972-1973. After that, three outbreaks burst between 1975 and 1990, caused successively by dengue types 1, 4 and 3 but from 1985, human strains of different types were isolated at the same time. The vector control measures, mainly aerial ULV spraying had a variable efficacy. The most important vector was Aedes aegypti but dengue virus strains were also isolated from other mosquito species (Aedes vigilax, Culex annulirostris, C. bitaeniorhynchus, Coquillettidia xanthogaster); their role in the interhuman transmission of dengue was not demonstrated. In New Caledonia, the development of international travels, an extension of suburbs and an increased insecticide resistance of Ae. aegypti are the obvious causes of the problem but an unknown transmission cycle, involving different hosts and vectors may also be suspected. 相似文献
993.
994.
P Lundy 《Canadian Metallurgical Quarterly》1996,42(3):313-324
Sociologists and, more recently, critical medical anthropologists have been arguing for a refocusing of the analysis of health and health care towards a perspective which considers the broader global political economy. In the context of the debt crisis and IMF/World Bank-inspired structural adjustment policies, the political economy theoretical perspective is becoming even more relevant in the analysis of health underdevelopment in many 'Third World' countries. This study focuses on the direct and indirect effects of the Jamaican debt crisis and structural adjustment programmes on health care services and health standards. In this paper it is argued that there are methodological problems using quantitative data when studying the effects of structural adjustment. In addition to providing a limited account of the effects, it is argued that the basic problem is a matter of the availability and reliability of the quantitative data in many 'Third World' countries. It is argued that some of these problems could be overcome by the application of qualitative micro-level analysis. This type of methodology is important to ascertain the effects of global processes at the grass roots level and to gain insights into what those working in the health sector are experiencing and what they perceive as the effects, if any, of structural adjustment policies. This has often been missing from the impersonal accounts offered by quantitative research on the subject to date. 相似文献
995.
996.
In order to protect Ni–Cr alloys from high-temperature corrosion, a new heat-resistant glass-ceramic coating was developed with a glass matrix synthesized on the basis of a composite R
x
O–Al2O3–SiO2–TiO2 (R–Li–, Na+, K+, Mg2+, Ca2+, Ba2+) system. The special features of the formation of crystalline phases in the glasses in heat treatment and the optimum regime for the formation of a glass ceramic structure are described.Translated from Steklo i Keramika, No. 3, pp. 30–32, March, 1996. 相似文献
997.
A. J. Kalkman H. P. M. Pellemans T. O. Klaassen W. Th. Wenckebach 《Journal of Infrared, Millimeter and Terahertz Waves》1996,17(3):569-577
The low temperature lifetime of electrons excited in the 2p?1 donor level of n-GaAs has been studied in a far-infrared pump-probe experiment. The measurement has been carried out using a pulsed far-infrared molecular gas laser working at a wavelength of 292µm, with the sample in a magnetic field of 5.1 T, resonant with the 1so?2p?1 transition. Two FIR pulses are sliced from one FIR-laser pulse by means of optical switching techniques using two Q-switched Nd:YAG lasers. The first pulse is used to saturate the transition, while the second pulse probes the return of the population in the excited state towards thermal equilibrium as a function of the time delay after the excitation pulse. The value of 350±50 ns found for the lifetime falls in line with CW saturation results on materials with other doping concentrations. 相似文献
998.
A Bousquet-Mélou J Galitzky M Lafontan M Berlan 《Canadian Metallurgical Quarterly》1995,36(3):451-461
The mechanisms that control lipolysis in intra-abdominal fat cells from various primate species, the marmoset (Callithrix jacchus), the baboon (Papio papio), and the macaque (Macaca fascicularis), were compared to those of human intraabdominal fat cells. Selective beta 1- or beta 2-adrenoceptor agonists induced lipolysis in all species. Selective beta 3-agonists (BRL 37344, CL 316243, and SR 58611) acted as partial agonists in marmoset but were inefficient in other primates, including humans. alpha 2-Adrenoceptor number ([3H]RX 8210002 binding) equalized (baboon) or exceeded (other primates) beta 1/beta 2-adrenoceptors ([3H]CGP 12177 binding). Baboon fat cell membranes expressed similar amounts of coupled beta- and alpha 2-adrenoceptors. In all species, norepinephrine- or epinephrine-induced lipolysis did not reach the lipolytic effect of isoproterenol but their effects were enhanced after alpha 2-adrenoceptor blockade. N6-phenylisopropyladenosine (PIA) induced a full antilipolytic effect in baboon, macaque, and human adipocytes through adenosine receptors ([3H]DPCPX binding). Peptide YY (PYY) weakly inhibited lipolysis in baboon. Adrenocorticotropic hormone (ACTH) was inactive whereas parathyroid hormone (PTH) partially stimulated lipolysis in primates. Histamine was partially lipolytic in marmoset only. This study emphasizes the similarities of the mechanisms controlling the lipolysis in nonhuman primate and in human adipocytes and suggests that the baboon and the macaque should provide unique models for the study of the regulation of lipolysis. 相似文献
999.
Winzker M. Gruger K. Gehrke W. Pirsch P. 《Solid-State Circuits, IEEE Journal of》1993,28(12):1354-1361
A chip set for 2D subband filtering of HDTV signals has been designed, fabricated and successfully tested. The two chips perform 10*14 quadrature mirror filtering for analysis filtering at the coder and synthesis filtering at the decoder. In order to achieve a very compact realization, the architectures utilize all a priori known properties of the filter algorithm. A 2D polyphase filter structure reduces the processing clock rate from the 72-MHz sampling rate to a moderate 18 MHz. The memory for vertical filtering is realized by on-chip parallel shift registers with multiphase clocking. A small silicon area for the filter arithmetic is achieved by application of carry save adder trees with fixed filter coefficients represented by canonical signed digits. A complete filterbank for luminance and chrominance signals consists of four identical chips, each with 450 000 transistors on 92 mm2 相似文献
1000.
The tone sense multiaccess with partial collision detection (TSMA/PCD) protocol is particularly suitable for a packet satellite system serving an area with a dense population of earth stations. By incorporating a narrowband ground radio channel for broadcasting busy ones, the earth stations are able to avoid packet collisions by sensing for the absence of busy tones before transmitting packets. Partial collision detection capability can also be achieved. Single-tone TSMA/PCD gives 97% of the carrier-sense multiaccess with collision detection (CSMA/CD) throughput when N =10 tones are used, while for multitone and slot-by-slot announcement TSMA/PCD protocols only N =8 and N =2, respectively, are sufficient to drive the system to the CSMA/CD performance 相似文献