Evidence that cell surface beta 1,4-galactosyltransferase spontaneously galactosylates an underlying laminin substrate during fibroblast migration

beta 1,4-Galactosyltransferase is unusual among the glycosyltransferases in that a subpopulation exists on the cell surface in addition to its traditional biosynthetic location within the Golgi complex. On the cell surface, galactosyltransferase is expressed in spatially restricted, cell type-specific domains, where it functions as a receptor for extracellular oligosaccharide ligands during selected cellular interactions. For example, galactosyltransferase is found on the leading and trailing edges of migrating cells, where it facilitates lamellipodia formation and cell spreading by binding to specific N-linked oligosaccharides within laminin. Although the ability of galactosyltransferase to serve as a laminin receptor is well documented, it is unclear whether it functions solely in a lectin-like capacity to bind laminin glycoside ligands or uses its intrinsic catalytic activity to release itself from and modify its oligosaccharide substrate. In this study, we determined whether cell surface galactosyltransferase spontaneously galactosylates laminin matrices during cell migration using endogenous galactose donors. Cells were prelabeled with [3H]galactose, washed, and transferred in small clusters onto laminin matrices. The prelabeled cells migrated out from the cell cluster, during which time they deposited covalently bound [3H]galactose residues onto the laminin matrix. The degree of galactosylation was both laminin- and time-dependent and required actively migrating, intact cells. The radioactivity released from the 3H-galactosylated laminin by acid hydrolysis comigrated with authentic galactose standards on paper chromatography. In parallel assays, there was no radioactivity deposited on laminin matrices when cells were prelabeled with [3H]fucose or [3H]leucine. Furthermore, [3H]galactosylation was dependent upon galactosyltransferase-mediated cell migration, since prelabeled cells did not deposit [3H]galactose when migrating on fibronectin, upon which migration is integrin-dependent and galactosyltransferase-independent. These results raise the possibility that galactosyltransferase functions catalytically during cell migration, either to dissociate from its oligosaccharide ligand and/or to modify the extracellular matrix.     

明清家具与牙板装饰纹样的形制特征

牙板是家具上具有结构功能的装饰构件。本文通过对明清家具牙板的形制与不同装饰纹样的形制进行分析,来研究牙板装饰纹样的形制,得出植物装饰纹样、动物装饰纹样和其他装饰纹样是牙板的主要形制特征。通过对牙板的深入的研究,使我们从一个全新的角度来重新认识明清家具,为明清家具牙板装饰艺术的系统化研究奠定基础。     

川西民间座椅类古旧家具的品种和用材

本文研究了以成都平原为中心的四川西部地区由当地木匠手工制作,供社会中下层使用、充满地方特色和乡土气息的民间座椅类古旧家具。具体介绍了川西民间座椅的常见品种,以及它们的用材特色,希望通过研究加深对川西家具文化的理解,促进家具地域文化的传承,同时对川西家具的现代制作和改良以及室内装饰设计等方面提供参考和借鉴。     

设计艺术专业教学实践平台构建研究——以景德镇陶瓷学院环境艺术设计专业实践教学为例

设计艺术专业是一门实际应用型专业,实践教学是非常重要的环节。本文针对高校设计艺术学专业的现状和培养目标,提出欲培养和提升教师及学生的实际动手能力,构建教学实践平台并以此拓宽教师和学生接触实践的渠道,是培养实际应用型人才的必然需求,也是有效途径。     

室内观赏植物的生态保健作用

概述了室内观赏植物的概念及常见种类,分析了室内观赏植物的生长环境以及栽种注意事项、室内植物的保健作用。     

设计创造生活便利——学生设计竞赛亮相芝加哥2010国际家居用品展

学生设计竞赛获奖作品及其各奖项得主亮相2010芝加哥国际家具用品展（International Homehouse Wares Show 2010）,无疑是本届展会上一个名副其实的亮点所在。由国际家居协会发起的学生设计大赛,今年已经是第17届了,获奖者给创新产品所下的定义就是：能帮助人们更加高效便利地生活。获奖作品包括多功能婴儿澡盆到烟火警报器,从储存食物到园艺工具的创新。     

Gia嘉奖世界家居零售业的创新者——2009～2010Gia年度大奖述评

2009年3月,我们曾在芝加哥的国际家居用品展上,参观了Gia2008～2009年度奖项的展示专区,对Gia有了一些感性认识,得到了一个不坏的＂第一印象＂;尔后,曾在本刊以《GIA评奖激励家居零售业创新》为题（2010年2、3期连载）作了报道。今年3月再度到访芝加哥参观Gia展区,并有幸应邀出席了Gia颁奖盛典的晚宴。值得一提的是,与我们同坐一席的马来西亚＂Jia（家）＂的公司代表,在与众多欧美企业的角逐中,捧得Gia奖杯,成为亚洲地区唯一获奖的企业,不折不扣地成为了颁奖盛宴中的亚洲亮点。