Implementation of response surface methodology to optimise extraction of onion (Allium cepa) solid waste phenolics

An experimental setup based on a 2³-full factorial, central composite design was implemented with the aim to optimising recovery of polyphenols from onion solid wastes (OSW). In order to allow for the establishment of a sustainable process, reusable and non-toxic solutions composed of water/ethanol/citric acid were employed as extracting media. The factors considered were (i) the pH of the medium, (ii) the extraction time and (iii) the ethanol concentration. The model obtained produced a satisfactory fitting of the experimental data with regard to total polyphenol extraction (R² = 0.97, p = 0.0025) and the reducing power of the extracts (R² = 0.97, p = 0.0033), but not with the antiradical activity (R² = 0.89, p = 0.0592). The 2nd order polynomial equations obtained after elaboration of the experimental data indicated that all parameters considered were significant in respect with the efficiency of total polyphenol recovery. The highest total polyphenol yield was theoretically predicted to be 9342 ± 1435 mg gallic acid equivalents per 100 g dry weight, under optimal conditions (60% EtOH, pH 2 and 4.2 h). Liquid chromatography-electrospray ionisation mass spectrometry of the optimally obtained extract revealed that the principal phytochemicals recovered were quercetin 3,4′-diglucoside, quercetin 4′-glucoside and quercetin. Simple linear regression analysis between the total polyphenol and the antiradical activity of the OSW extracts showed that there was no correlation in a statistically significant manner, as opposed to reducing power.Industrial relevanceThe recovery of value-added substances from agri-food industrial wastes is an issue with importance pertaining to both the reduction of the waste load released to the environment, and the development of novel, natural food additives with functional properties. Up to date, the examinations pertaining to the clarification of factors that can affect extractability were based on rather unilateral assessment, while it is generally accepted that the retrieval depends on several parameters, which render the phenomenon a particular complexity. In this view, the implementation of factorial design with respect to investigating in parallel several factors pertaining to efficient polyphenol recovery becomes imminent. Thus the establishment of models on such a sound experimental basis is expected to provide a reliable background for more costand resource-effective processes, with a potential direct industrial applicability.     

Model for Understanding, Preventing, and Resolving Project Disputes

Based on the comparative analysis of 24 construction disputes, this paper presents a process model that explains the development of disputes. The model examines the combined effect of project uncertainty, contract, working relations, and problem solving effectiveness on the development of disputes. The model develops a classification of problem situations, and identifies the problem-solving requirements and the potential for dispute in each situation. The model indicates that the prevention of complex, high cost disputes depends more on the planning and problem solving ability of the project organization, and less on the contractual terms. The paper identifies the following actions that can reduce the number and severity of claims: reduction of project uncertainty, reduction of contractual problems, reduction of opportunistic behavior, increased the project organization's problem-solving ability, and use of alternative dispute resolution methods to reduce resolution costs.     

A full‐scale system for aerobic biological treatment of olive mill wastewater

Olive mill wastewater (OMW) is a major environmental problem in the Mediterranean basin. Although many methods for OMW treatment have been developed, only a few have been adopted in pilot‐ or full‐scale applications. A full‐scale system for aerobic biological treatment of OMW was developed. The system consists of a trickling filter and a recirculation tank. Continuous recirculation of the wastewater was used to provide oxygen concentrations from 0.7 to 1.2 mg L^?1. Low ambient temperatures did not affect system performance since the raw wastewater was warm enough. Nutrient addition was not necessary as raw wastewater contained sufficient nitrogen and phosphorous concentrations. Indigenous olive pulp bacteria proved to be resistant to full‐scale conditions. Feed chemical oxygen demand and phenolic concentrations were about 43 000 and 9500 mg L^?1, respectively. The system reduced more than half of the organic load under continuous operation and a hydraulic retention time of 24 h. The efficiency of this method could be improved by combining it with another technology to further reduce the organic load. The absence of mechanical aeration and the very low hydraulic retention time denotes that the proposed system could be viable and attractive. Copyright © 2011 Society of Chemical Industry     

Setting process of lime-based conservation mortars with barium hydroxide

This paper presents the effect of barium hydroxide on the setting mechanism of lime-based conservation mortars, when used as an additive material. The study focuses on the monitoring of the setting process and the identification of the mineral phases formed, which are essential for furthering the study of the durability of barium mixtures against chemical degradation. X-ray diffraction analysis (XRD), scanning electron microscopy (SEM) and thermal analysis (DTA-TG) were used to monitor the setting processes of these mixtures and identify new phases formed. The results suggest that barium hydroxide is evenly distributed within the lime and produces a homogeneous binding material, consisting of calcite (CaCO₃), witherite (BaCO₃) and barium-calcium carbonate [BaCa(CO₃)₂]. Finally, it was found that barium carbonate can be directly bonded to calcitic aggregates and therefore increases its chemical compatibility with the binding material.     

Radical-scavenging activity of Aegean Sea marine algae

In the present work, thirteen algae from the Aegean Sea were examined for radical-scavenging activity (RSA) using the DPPH and chemiluminescence (CL) tests. Extracts of the brown alga Taonia atomaria exhibited the best RSA in comparison to the extracts of the other investigated species and approached the activity of powerful antioxidant standards. Column chromatography separation of T. atomaria extract, followed by preparative HPLC, resulted in the isolation of six metabolites, which were identified by spectral analyses (¹H NMR, ¹³C NMR and MS). The isolated metabolites taondiol, isoepitaondiol, stypodiol, stypoldione, sargaquinone and sargaol were found to possess marked RSA.     

Theoretical and experimental investigation of pulsed laser grooving process

A theoretical model has been developed for simulating the laser grooving process. It takes into account the interaction among subsequent pulses, the required time for the melting temperature to be reached and the subsequent removal of a finite volume of material during each laser pulse. The model predicts the maximum groove depth that can be achieved for a specified set of process parameters, such as laser power, pulsing frequency, and scanning velocity. The theoretical predictions have been experimentally tested with a medium-power laser beam.     

Use of lambda DNA as a marker to assess DNA stability in olive oil during storage

Filtered olive oil samples spiked with three different concentrations of λDNA were stored at 25 °C under a 12 h photoperiod for up to a year. These samples were used for DNA extraction and PCR amplification of λDNA amplicons of 107, 415 and 691 bp length. The amplification signal was gradually decreased with longer storage periods, while the strength of the signal was related to the initial concentration of spiking λDNA particularly during longer storage periods. The 107 bp amplicon was the only one successfully amplified from all the samples, regardless of both concentration of spiking λDNA and storage period. The amplification of 415 and 691 bp amplicons was not successful for samples stored longer than a threshold period of 20 and 10 days, respectively. These results suggest that detection of polymorphic markers requiring DNA templates shorter than 100 bp might have a wider range of applications in DNA fingerprinting of olive oil. In addition, the DNA extracts were tested for the presence of inhibitors in PCR amplification reactions of yeast DNA amplicons. The inhibitory effect of olive DNA extracts was partial and gradually increased with the storage period of the olive oil samples used for the DNA extraction.     

Forces Driving Adoption of New Information Technologies

This paper investigates the forces that drive construction firms to adopt new information technologies. The research focuses on CAD and electronic data interchange technologies and studies in-depth eight innovation adoption decisions. The paper presents the findings regarding the forces that created the managerial stimuli for innovation and the organizational characteristics that played an important role in the adoption. The investigation identified four forces that drive innovation: competitive advantage, process problems, technological opportunity, and institutional requirements. These forces change over time and drive the diffusion of a technology in the industry. The study also found that different organizational characteristics determine a company's sensitivity to each force. The paper proposes a new model of diffusion of new technologies and presents the implications for increasing the rate of innovation in the industry.     

MRE hierarchical decomposition of general queueing network models

Summary The principle of Minimum Relative Entropy (MRE), given fully decomposable subset and aggregate mean queue length, utilisation and flow-balance constraints, is used in conjunction with asymptotic connections to infinite capacity queues, to derive new analytic approximations for the conditional and marginal state probabilities of single class general closed queueing network models (QNMs) in the context of a multilevel variable aggregation scheme. The concept of subparallelism is applied to preserve the flow conservation and a universal MRE hierarchical decomposition algorithm is proposed for the approximate analysis of arbitrary closed queueing networks with single server queues and general service-times. Heuristic criteria towards an optimal coupling of network's units at each level of aggregation are suggested. As an illustration, the MRE algorithm is implemented iteratively by using the Generalised Exponential (GE) distributional model to approximate the service and asymptotic flow processes in the network. This algorithm captures the exact solution of separable queueing networks, while for general queueing networks it compares favourably against exact solutions and known approximations.This work is sponsored by the Science and Engineering Research Council (SERC), UK, under grant GR/F29271