Designed amyloid beta peptide fibril - a tool for high-throughput screening of fibril inhibitors

Amyloid beta peptide (Abeta) fibril formation is widely believed to be the causative event of Alzheimer's disease pathogenesis. Therapeutic approaches are therefore in development that target various sites in the production and aggregation of Abeta. Herein we present a high-throughput screening tool to generate novel hit compounds that block Abeta fibril formation. This tool is an application for our fibril model (Abeta(16-37)Y(20)K(22)K(24))(4), which is a covalent assembly of four Abeta fragments. With this tool, screening studies are complete within one hour, as opposed to days with native Abeta(1-40). A Z' factor of 0.84+/-0.03 was determined for fibril formation and inhibition, followed by the reporter molecule thioflavin T. Herein we also describe the analysis of a broad range of reported inhibitors and non-inhibitors of Abeta fibril formation to test the validity of the system.     

Evaluation of performance improvement by model predictive control in a renewable energy system with hydrogen storage

Nowadays islanded microgrids mostly rely on diesel generator. In order to reduce greenhouse emissions, two islanded microgrids with hydrogen storage have been installed and are currently working autonomously in Reunion Island and France. Energy management implemented on these stations fall in the myopic control category. This study aims to determine the performance improvement that could be achieve on such stations using model predictive control. Ability to supply to the loads and energy losses minimisation are our main objectives. Lifetime degradation is also taken in consideration. Simulations shows that significant improvement can be brought, with a 76% decrease of the station's defaults time and better fill rate of hydrogen tank and batteries. In the meantime, chemical and electrical losses are reduced by 38 and 11%, and the batteries degradations are decreased by around 1%. The relevance of taking into account the electrolyser and fuel cell degradations depends on the time-step of the control. These results give a performance target in order to implement a real-time model predictive control in the microgrids, and eventually can be used to better sizing of future microgrids with similar architecture.     

Experimental investigation in the TOSQAN facility of heat and mass transfers in a spray for containment application

TOSQAN is an experimental program undertaken by the Institut de Radioprotection et de Sûreté Nucléaire (IRSN) in order to perform thermal hydraulic containment studies. The TOSQAN facility is a large enclosure devoted to simulate typical accidental thermal hydraulic flow conditions in nuclear-pressurized water reactor (PWR) containment. The TOSQAN facility which is highly instrumented with non-intrusive optical diagnostics is particularly adapted to nuclear safety CFD code validation. The present work is devoted to studying the interaction of a water spray injection used as a mitigation means in order to reduce the gas pressure and temperature in the containment, to produce gases mixing and washout of fission products. In order to have a better understanding of heat and mass transfers between spray droplets and the gas mixture, and to analyze mixing effects due to spray activation, we perform detailed characterization of the two-phase flow.     

Polystyrene latex particles bearing primary amine groups via soap‐free emulsion polymerization

Polystyrene latexes were prepared in the presence of an amino‐containing functional comonomer, N‐(3‐aminopropyl)methacrylamide hydrochloride (APMH), via soap‐free batch emulsion polymerization initiated by the cationic initiator 2,2′‐azobis(2‐amidinopropane) dihydrochloride. These latexes were characterized by studying the influence of the ionic comonomers on the polymerization kinetics, particle size, surface charge density and colloidal properties. The synthesized latexes were monodisperse with a final size between 100 and 600 nm depending on the APMH concentration. The initial polymerization rate and the particle number increased in accordance with the Smith–Ewart theory for soap‐free styrene emulsion polymerization with a hydrophilic functional comonomer. The final functionalization rate of the particles has been particularly studied with the intention of fitting the prepared latexes to be used in the immobilization of biological molecules for biological sample preparation and diagnostic applications. © 2020 Society of Chemical Industry     

Antimicrobial Activity of Nisin and Natamycin Incorporated Sodium Caseinate Extrusion‐Blown Films: A Comparative Study with Heat‐Pressed/Solution Cast Films

Antimicrobial edible films based on sodium caseinate, glycerol, and 2 food preservatives (nisin or natamycin) were prepared by classical thermomechanical processes. Food preservatives were compounded (at 65 °C for 2.5 min) with sodium caseinate in a twin‐screw extruder. Anti‐Listeria activity assays revealed a partial inactivation of nisin following compounding. Thermoplastic pellets containing food preservatives were then used to manufacture films either by blown‐film extrusion process or by heat‐press. After 24 h of incubation on agar plates, the diameters of K. rhizophila growth inhibition zones around nisin‐incorporated films prepared by solution casting (control), extrusion blowing or heat pressing at 80 °C for 7 min of nisin‐containing pellets were 15.5 ± 0.9, 9.8 ± 0.2, and 8.6 ± 1.0 mm, respectively. Since heat‐pressing for 7 min at 80 °C of nisin‐incorporated pellets did not further inactivate nisin, this indicates that nisin inactivation during extrusion‐blowing was limited. Moreover, the lower diameter of the K. rhizophila growth inhibition zone around films prepared with nisin‐containing pellets compared to that observed around films directly prepared by solution casting confirms that nisin inactivation mainly occurred during the compounding step. Natamycin‐containing thermoplastic films inhibited Aspergillus niger growth; however, by contrast with nisin‐containing films, heat‐pressed films had higher inhibition zone diameters than blown films, therefore suggesting a partial inactivation of natamycin during extrusion‐blowing.     

卫星高光谱红外大气探测的正演模拟研究

利用精确的红外大气辐射传输计算模型KCARTA,模拟计算了光谱分辨率、通道光谱响应函数、光谱定标精度、大气倾斜路径和表面发射率对高光谱观测亮温的影响.正演计算的结果表明：1）提高光谱分辨率明显增加了可探测的大气亮温;2）各种内、外部因素的变化对高光谱仪器将产生远较低光谱仪器更大的观测亮温差.本研究可为我国高光谱大气探测仪器的设计以及高光谱大气探测反演算法研究的开展提供科学的参考依据.     

便携式电子设备中的立体声

手机等便携式电子产品正集成越来越多的多媒体功能，这引起了人们对增加立体声功能的极大兴趣。单声道结构用于声音放大及和弦铃声，在某些情况下可用作免提扬声器。虽然功能已足够，但这并非是令人满意的高质量音乐播放解决方案。     

Probing photonic and optoelectronic structures by apertureless scanning near-field optical microscopy

This report presents the Apertureless Scanning Optical Near-Field Microscope as a powerful tool for the characterization of modern optoelectronic and photonic components with sub-wavelength resolution. We present an overview of the results we obtained in our laboratory over the past few years. By significant examples, it is shown that this specific probe microscopy allows for in situ local quantitative study of semiconductor lasers in operation, integrated optical waveguides produced by ion exchange (single channel or Y junction), and photonic structures.     

Nitrogen contributions of alley cropped Trifolium pratense may sustain short rotation woody crop yields on marginal lands

Nutrient Cycling in Agroecosystems - Incorporating nitrogen-fixing cover crops into short rotation woody crop (SWRC) plantations may increase soil and tree nitrogen while sustaining, or even...