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111.
Normal human peripheral blood mononuclear cells (MNCs), particularly T lymphocytes (T cells), are a rich source of granulocyte-macrophage colony-stimulating factor (GM-CSF). Glucocorticoids are known to inhibit GM-CSF production in in vitro cultures of a human fibroblast cell line and in normal human blood monocytes and alveolar macrophages. To determine whether glucocorticoids also inhibit GM-CSF production from normal human MNCs and T cells, we set up cultures of normal human MNCs and T cells in a liquid system in the presence and absence of 5, 50, and 250 microg/dL of hydrocortisone, and an hour later, a constant dose of 50-ng/mL Escherichia coli lipopolysaccharide (LPS) or 10-microg/mL phytohemagglutinin (PHA) was added. After three days, cell counts and GM-CSF levels were determined. Administering 50- and 250-microg/dL hydrocortisone decreased lymphocyte recovery from MNC cultures with LPS (p < or = 0.01), and 250 microg/dL of hydrocortisone decreased lymphocyte recovery from MNC and T-cell cultures with PHA (p < or = 0.03). The amount of GM-CSF produced from PHA-stimulated MNCs was about 100-fold higher than that produced from LPS-stimulated MNCs. The magnitude of GM-CSFs produced in MNC and T-cell cultures stimulated by PHA was comparable (p=0.88). Administering hydrocortisone at 5, 50, and 250 pg/dL decreased GM-CSF production (p < 0.003) in LPS- or PHA-stimulated MNC cultures and in PHA-stimulated T-cell cultures. PHA (not tested with LPS)-stimulated GM-CSF messenger RNA (mRNA) expression was blocked by hydrocortisone. These results indicate that lower concentrations of hydrocortisone inhibit GM-CSF production from normal human blood MNCs and T cells entirely by inhibiting the expression of GM-CSF mRNA, and higher concentrations of hydrocortisone inhibit by a combined effect of inhibiting the expression of GM-CSF mRNA and decreasing the lymphocyte count. 相似文献
112.
Pasquale Daponte 《Measurement》1995,15(4):213-222
The behaviour of electronically compensated current transformers is examined with reference to a model valid for frequencies ranging from industrial to ultra-acoustic both in steady-state and in transient conditions. The influence of the hysteresis and saturation effects is evidenced. A comparison with other proposed current transducers is also given. A quantitative treatment of the errors is given and a good agreement between simulation results and experimental data is obtained. 相似文献
113.
R Lorenzoni D Pagano G Mazzotta SD Rosen G Fattore R De Caterina G Gensini 《Canadian Metallurgical Quarterly》1998,19(10):1518-1524
BACKGROUND: The economic evaluation of the results of the Global Utilization of Streptokinase and Tissue Plasminogen Activator for Occluded Coronary Artery (GUSTO) trial found that recombinant tissue plasminogen activator is more cost-effective than streptokinase for the treatment of acute myocardial infarction. AIM: We evaluated the impact on a cost effectiveness analysis, of the differences in the cost of thrombolytics among countries and of differences in efficacy across patient subgroups. METHODS: We considered the crude costs of streptokinase and recombinant tissue plasminogen activator in Germany, Italy, the United Kingdom, and the United States of America, and the 30-day mortality found in the GUSTO trial. We calculated the incremental costs for each life saved when streptokinase is substituted by recombinant tissue plasminogen activator. We also calculated the incremental costs for each life saved for two protocols implying a selective use of streptokinase and recombinant tissue plasminogen activator (age-selective protocol: recombinant tissue plasminogen activator in patients < or = 75 years, streptokinase in older patients; site-selective protocol: recombinant tissue plasminogen activator in anterior acute myocardial infarction, streptokinase in non-anterior acute myocardial infarction). RESULTS: The incremental costs for each life saved when streptokinase is substituted by recombinant tissue plasminogen activator in all GUSTO patients vary greatly among countries: the incremental costs for each life saved are 31%, 45%, and 97% higher in Germany, Italy, and the United States of America compared to the United Kingdom. The use of a site-selective protocol implies a halved cost-effectiveness ratio compared to the use of recombinant tissue plasminogen activator in all cases of acute myocardial infarction. CONCLUSIONS: (1) The cost-efficacy of recombinant tissue plasminogen activator vs streptokinase in acute myocardial infarction varies greatly among countries due to differences in the cost of drugs. (2) A selective use of thrombolytics for some sites of infarction is more cost-effective than the exclusive use of recombinant tissue plasminogen activator. 相似文献
114.
A carefully controlled comparative study showed miconazole nitrate 2% vaginal cream (Monistat) to be a highly effective agent in the treatment of vaginal candidiasis in pregnant subjects. Miconazole nitrate was significantly more effective than nystatin (Mycostatin) in the treatment of vaginal candidiasis in all three trimesters of pregnancy, and also more effective regardless of whether the candidal infection was primary or recurrent. Observations relating to the safety of this therapy during pregnancy were made and discussed. 相似文献
115.
Suboptimal supervisory control of Petri nets in presence of uncontrollable transitions via monitor places 总被引:1,自引:0,他引:1
Francesco Basile Author Vitae Pasquale Chiacchio Author Vitae Author Vitae 《Automatica》2006,42(6):995-1004
This paper deals with the problem of enforcing generalized mutual exclusion constraints (GMEC) on place/transition nets with uncontrollable transitions. An efficient control synthesis technique, which has been proposed in the literature, enforces GMEC constraints by introducing monitor places to create suitable place invariants. The method has been shown to be maximally permissive and to give a unique control structure in the case that the set of legal markings is controllable. This paper investigates on and formally shows that the class of controllers obtained by this technique may not have a supremal element for uncontrollable specifications. Moreover, it is shown that the family of monitor places enforcing an uncontrollable specification can be parameterized with respect to the solution of a linear system of equation. An algorithm to obtain such parameterization is presented here. 相似文献
116.
Study of in-bin canola (rapeseed) drying with near-ambient air is realised to know characteristics of drying and seed deterioration. Simulation models are applied to estimate both drying and in-bin cooling times. Along the process: (a) bed-density increases; (b) porosity varies with a second-order relation of time; (c) bed height decreases, mainly during the first 27 drying hours; (d) was not visually observed damages on grain surface; (e) neither was observed appreciable changes on the quality parameters refraction index, performance of oil extraction, acidity index and germination power; (f) drying time can be predicted through the programmable simulator designed to drying in-bin of shelled corn and other grains (Bauer et al., 1980[2]) with error lesser than 2.2%; (g) in-bin cooling time (17 h) was comparable with data of literature extrapolated from Christensen model (1982)[4], which predicts 14 h for cooling-time. 相似文献
117.
Vachaspathi P. Kompella Joseph C. Pasquale George C. Polyzos 《Journal of Network and Systems Management》1996,4(2):107-131
We consider the problem of optimal multicast routing with Quality of Service constraints motivated by the requirements of interactive continuous media communication, e.g., real-time teleconferencing. We concentrate on distributed algorithms for determining a tree over the network topology, rooted at the source and spanning the intended destinations. Quality of Service requirements for interactive continuous media typically impose constraints on some metric over the individual paths from the source to each destination, usually in the form of an upper bound on the delay. Thus, we focus on the problem of minimizing the cost of the tree while at the same time satisfying a common constraint over individual source-destination paths. We have shown that this problem is intractable, but have also devised centralized polynomial time heuristics that perform well. Here we present distributed algorithms to minimize tree cost while satisfying the constraints on the paths from the source to each destination. 相似文献
118.
Antonio?PigaEmail author Pasquale?Catzeddu Stefano?Farris Tonina?Roggio Annamaria?Sanguinetti Efisio?Scano 《European Food Research and Technology》2005,221(3-4):387-391
The results of a study on texture evolution during 35 days of storage of amaretti, a typical Italian cookie, packaged in two different ways are reported. Amaretti cookies were wrapped in polyvinylchloride (PVC) film or aluminium foil (ALL), to simulate two different permeability conditions and stored at controlled temperature and humidity. Evolution of texture (such as hardness) and aw were tested instrumentally by a texture analyser and a hygrometer, respectively. Texture was assessed by a cut and puncturing test. Indices for hardening were the area under the curve (N mm) and gradient (N/mm) for the puncturing test and maximum force (N) for the cut test. Both textural tests showed significantly higher hardening of PVC cookies, compared to the ALL cookies. The latter retained good sensorial properties at the end of the storage period, although their internal structure changed from soft and moist to mealy, while the PVC cookies were no longer edible only 10 days after baking. aw values decreased and increased in PVC and ALL lots, respectively. The results suggest that hardening may be explained by water loss in PVC and moisture redistribution in ALL. 相似文献
119.
We analyze, in the framework of the functional approach to statistical mechanics, general properties of correlation functions in ordered magnetic systems. The aim of this paper is to investigate what consequences can be derived from the requirement of invariance of the theory under local infinitesimal spin rotation. The extension of the method that we introduce, to include anisotropic Hamiltonians, is discussed, and the case of the anisotropy described by an internal field is worked out. Our main results are a set of generalized Ward identities, a microscopic definition of the stiffness constant as a static limit of a local response function, and an exact asymptotic expression for the correlation functions of interest, for zero temperature and small wave length and frequency. Modifications to the hydrodynamic expression for correlation functions in the case where anisotropy is present are also discussed. 相似文献
120.