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131.
Prostaglandins act through specific receptors to stimulate cyclic AMP formation which inhibits platelet activation and relaxes vascular smooth muscle. We have used RT-PCR combined with Southern blot analysis to determine the subtypes of prostaglandin receptor on platelets. Platelets expressed the EP4 rather than the EP2 prostaglandin EP receptor subtype, whereas vascular smooth muscle cells predominantly expressed the EP2 receptor. The IP receptor, which binds prostacyclin and couples to stimulation of adenylyl cyclase, and three isoforms of the inhibitory EP3 receptor were equally expressed in platelets, HEL cells and umbilical artery smooth muscle cells. The EP3-II isoform showed variation in level of expression among the three cell types. As a positive control for the presence of platelet RNA, PCR was performed using primers specific for the alpha chain of the platelet membrane glycoprotein Ib. As a negative control for the absence of T and B cell contamination in the platelet RNA, PCR was performed using primers specific for the cell specific cluster determinants CD2 (a T-cell marker) and CD20 (a B-cell marker). The finding that platelets express both stimulatory and inhibitory prostaglandin receptors provides confirmation of a homeostatic model of regulation of platelet adenylyl cyclase previously proposed. 相似文献
132.
Edwards Jean M.; Rudisill John R.; Champney Tim F.; Hershberger Paul J.; Polaine Vanessa H.; Archambault Denise L. 《Canadian Metallurgical Quarterly》1998,50(3):173
Outplacement firms developed in response to corporate downsizings to assist former upper-level employees in seeking reemployment. An important question for practitioners who assist clients in outplacement is how the personal characteristics that clients bring to the situation influence their success in finding a new job. The present archival study examined demographic variables, previous job history, and personality characteristics as predictors of outplacement outcomes. Outplacement duration was predicted by previous income, and new earnings were predicted by previous earnings, sex, and years with previous employer. The need for prospective, longitudinal research that examines the coping process in outplacement is discussed. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
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This paper is concerned with improvement in optical image quality by image restoration. Image restoration is an ill-posed
inverse problem which involves the removal or minimization of degradations caused by noise and blur in an image, resulting
from, in this case, imaging through a medium. Our work here concerns the use of the underlying Toeplitz structure of such
problems, and associated techniques for accelerating the convergence of iterative image restoration computations. Denoising
methods, including total variation minimization, followed by segmentation-based preconditioning methods for minimum residual
conjugate gradient iterations, are investigated. Regularization is accomplished by segmenting the image into (smooth) segments
and varying the preconditioners across the segments. By taking advantage of the Toeplitz structure, our algorithms can be
implemented with computational complexity of onlyO (ln
2 logn), wheren
2 is the number of pixels in the image andl is the number of segments used. Also, parallelization is straightforward. Numerical tests are reported for atmospheric imaging
problems, including the case of spatially varying blur.
Research supported in part by a National Science Foundation Postdoctoral Research Fellowship.
Research sponsored by the U.S. Air Force Office of Scientific Research under grant F49620-97-1-1039.
Research sponsored by the U.S. Air Force Office of Scientific Research under grant F49620-97-1-0139, and by the National Science
Foundation under grant CCR-96-23356.
Research sponsored by the National Science Foundation under grant CCR-96-23356. 相似文献
137.
The problem of packing circles into a domain of prescribed topology is considered. The circles need not have equal radii. The Collins-Stephenson algorithm computes such a circle packing. This algorithm is parallelized in two different ways and its performance is reported for a triangular, planar domain test case. The implementation uses the highly parallel graphics processing unit (GPU) on commodity hardware. The speedups so achieved are discussed based on a number of experiments. 相似文献
138.
Sample preparation for DNA and RNA assays is a prime candidate for laboratory automation. A novel, parallel processing device that performs the three separate liquid-handling functions necessary for such sample preparation-dispensing, pipetting, and pressurizing-is presented. The device comprises an array of fine nozzles connected by fluidic channels to automatically and precisely distribute flow between one source and an array of points. The design principles, as well as the experimental and computational methods used to develop the device, are described. Test results, including accuracy, uniformity, volume range, and timing, are presented. The functionality of the device is demonstrated by performing a solid-phase extraction of DNA with two types of microcolumns. 相似文献
139.
Miguel Eduardo L. Felismino Paul A. Helm Chelsea M. Rochman 《Journal of Great Lakes research》2021,47(1):180-189
Microplastics (plastic particles <5 mm in size) have been reported in ecosystems worldwide and have been shown to cause adverse negative impacts on organisms. This study provides the first report of microplastics and other anthropogenic microparticles in the surface waters and sediments of Lake Simcoe in Ontario, Canada, a popular recreational and fishing lake. Surface waters (low volume grabs and manta trawls) and sediments were sampled from eight sites to determine microparticle abundances and character (size, morphology, material identity). Concentrations ranged from 0 to 0.7 particles/L in surface water grab samples, 0.4–1.3 particles/m3 in manta trawl samples, and 8.3–1070 particles/kg in sediment samples. Spectroscopic analysis confirmed that 72% of particles were anthropogenic, with 64% unambiguously identified as microplastics. However, confirmed microplastic concentrations were approximately 6–7 times lower than unadjusted counts in sediments, demonstrating the importance of verifying and adjusting reported values. Fibers were only quantified and characterized in surface water grab samples and sediments, and were the most common morphology, accounting for 82% and 89% of anthropogenic particles, respectively. Fragments were most common in manta trawl samples (75%, excluding fibers) and consisted predominantly of polyethylene (41%) and polypropylene (22%). The influence of proximity to urban centers and prevailing winds on the distribution of microplastics was apparent in surface water manta trawls. Microplastics and other anthropogenic microparticles are present in Lake Simcoe but at lower abundances than at locations impacted by larger population centers in other large freshwater lakes such as Lakes Ontario and Erie of the Great Lakes. 相似文献
140.