Characterization of Early Enzymes Involved in TDP‐Aminodideoxypentose Biosynthesis en Route to Indolocarbazole AT2433

The characterization of TDP‐α‐d ‐glucose dehydrogenase (AtmS8), TDP‐α‐d ‐glucuronic acid decarboxylase (AtmS9), and TDP‐4‐keto‐α‐d ‐xylose 2,3‐dehydratase (AtmS14), involved in Actinomadura melliaura AT2433 aminodideoxypentose biosynthesis, is reported. This study provides the first biochemical evidence that both deoxypentose and deoxyhexose biosynthetic pathways share common strategies for sugar 2,3‐dehydration/reduction and implicates the sugar nucleotide base specificity of AtmS14 as a potential mechanism for sugar nucleotide commitment to secondary metabolism. In addition, a re‐evaluation of the AtmS9 homologue involved in calicheamicin aminodeoxypentose biosynthesis (CalS9) reveals that CalS9 catalyzes UDP‐4‐keto‐α‐d ‐xylose as the predominant product, rather than UDP‐α‐d ‐xylose as previously reported. Cumulatively, this work provides additional fundamental insights regarding the biosynthesis of novel pentoses attached to complex bacterial secondary metabolites.     

The route to a defect tolerant LUT through artificial evolution

Evolutionary techniques may be applied to search for specific structures or functions, as specified in the fitness function. This paper addresses the challenge of finding an appropriate fitness function when searching for generic rather than specific structures which, when combined wiacteristic of defect tolerance on the circuit. Production defects for integrated circuits are expected to increase considerably. To avoid a corresponding drop in yield, improved defect tolerance solutions are needed. In the case of Field Programmable Gate Arrays (FPGAs), the pre-designed gate array provides a bridge between production and the application designers. Thus, introduction of defect tolerant techniques to the FPGA itself could provide a defect free gate array to the application designer, despite production defects. The search for defect tolerance presented herein is directed at finding defect tolerant structures for an important building block of FPGAs: Look-Up Tables (LUTs). Two key approaches are presented: (1) applying evolved generic building blocks to a traditional LUT design and (2) evolving the LUT design directly. The results highlight the fact that evolved generic defect tolerant structures can contribute to highly reliable circuit designs at the expense of area usage. Further, they show that applying such a technique, rather than direct evolution, has benefits with respect to evolvability of larger circuits, again at the expense of area usage.     

Efficient Boustrophedon Multi-Robot Coverage: an algorithmic approach

This paper presents algorithmic solutions for the complete coverage path planning problem using a team of mobile robots. Multiple robots decrease the time to complete the coverage, but maximal efficiency is only achieved if the number of regions covered multiple times is minimized. A set of multi-robot coverage algorithms is presented that minimize repeat coverage. The algorithms use the same planar cell-based decomposition as the Boustrophedon single robot coverage algorithm, but provide extensions to handle how robots cover a single cell, and how robots are allocated among cells. Specifically, for the coverage task our choice of multi-robot policy strongly depends on the type of communication that exists between the robots. When the robots operate under the line-of-sight communication restriction, keeping them as a team helps to minimize repeat coverage. When communication between the robots is available without any restrictions, the robots are initially distributed through space, and each one is allocated a virtually-bounded area to cover. A greedy auction mechanism is used for task/cell allocation among the robots. Experimental results from different simulated and real environments that illustrate our approach for different communication conditions are presented.     

A detailed description of the short-term musculoskeletal and cognitive effects of prolonged standing for office computer work

Due to concerns about excessive sedentary exposure for office workers, alternate work positions such as standing are being trialled. However, prolonged standing may have health and productivity impacts, which this study assessed. Twenty adult participants undertook two hours of laboratory-based standing computer work to investigate changes in discomfort and cognitive function, along with muscle fatigue, movement, lower limb swelling and mental state. Over time, discomfort increased in all body areas (total body IRR [95% confidence interval]: 1.47[1.36–1.59]). Sustained attention reaction time (β = 18.25[8.00–28.51]) deteriorated, while creative problem solving improved (β = 0.89[0.29–1.49]). There was no change in erector spinae, rectus femoris, biceps femoris or tibialis anterior muscle fatigue; low back angle changed towards less lordosis, pelvis movement increased, lower limb swelling increased and mental state decreased. Body discomfort was positively correlated with mental state. The observed changes suggest replacing office work sitting with standing should be done with caution.

Practitioner Summary: Standing is being used to replace sitting by office workers; however, there are health risks associated with prolonged standing. In a laboratory study involving 2 h prolonged standing discomfort increased (all body areas), reaction time and mental state deteriorated while creative problem-solving improved. Prolonged standing should be undertaken with caution.     

Hybrid dynamical systems with hybrid inputs: Definition of solutions and applications to interconnections

In this paper, we define solutions for hybrid systems with prespecified hybrid inputs. Unlike previous work where solutions and inputs are assumed to be defined on the same domain a priori, we consider the case where intervals of flow and jump times of the input are not necessarily synchronized with those of the state trajectory. This happens in particular when the input is the output of another hybrid system, for instance, in the context of observer design or reference tracking. The proposed approach relies on reparametrizing the jumps of the input in order to write it on a common domain. The solutions then consist of a pair made of the state trajectory and the reparametrized input. Our definition generalizes the notions of solutions of continuous‐time and discrete‐time systems with inputs. We provide an algorithm that automatically performs the construction of solutions for a given hybrid input. In the context of hybrid interconnections, we show how the solutions of the individual systems can be linked to the solutions of a closed‐loop system. Example illustrate the notions and the proposed algorithm.     

Adaptive output-feedback stabilization of non-local hyperbolic PDEs

We address the problem of adaptive output-feedback stabilization of general first-order hyperbolic partial integro-differential equations (PIDE). Such systems are also referred to as PDEs with non-local (in space) terms. We apply control at one boundary, take measurements on the other boundary, and allow the system’s functional coefficients to be unknown. To deal with the absence of both full-state measurement and parameter knowledge, we introduce a pre-transformation (which happens to be based on backstepping) of the system into an observer canonical form. In that form, the problem of adaptive observer design becomes tractable. Both the parameter estimator and the control law employ only the input and output signals (and their histories over one unit of time). Prior to presenting the adaptive design, we present the non-adaptive/baseline controller, which is novel in its own right and facilitates the understanding of the more complex, adaptive system. The parameter estimator is of the gradient type, based on a parametric model in the form of an integral equation relating delayed values of the input and output. For the closed-loop system we establish boundedness of all signals, pointwise in space and time, and convergence of the PDE state to zero pointwise in space. We illustrate our result with a simulation.     

Path Planning and Control for Free-Flying Inspection Robot in Space

This paper describes a prototype robot called AERCam and the necessary path planning and control for its space inspection applications. The robot is the first generation of a free-flying robotic camera that will assist astronauts in constructing and maintaining the space station. The robot will provide remote views both to astronauts inside the space shuttle and the future space station and to ground controllers. The first part of the paper describes a planar robot prototype autonomously moving about an air-bearing table. The second part of this paper introduces a method for determining paths in three dimensions for efficient fuel use. Finally, this paper describes the software simulation of the path planner with the future space station.     

Case for Undergraduate Study of Disasters

Literature on disasters provides the focus for student-based learning exercises in civil and architectural engineering at Queen's University, Belfast, which have been ongoing since 1994. Incorporating these studies in the early part of the curriculum helps students to reorientate from their previous mainly science-based study by gaining some knowledge of the roles, responsibilities, priorities, and work practices of engineers in a way that would not be possible through lectures. In view of the influence of disasters on engineering thinking and work practice, they should have a much larger influence than they currently do on curriculum development.