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91.
Dmitry V. Volodkin Stephan Schmidt Paulo Fernandes Natalia I. Larionova Gleb B. Sukhorukov Claus Duschl Helmuth Möhwald Regine von Klitzing 《Advanced functional materials》2012,22(9):1914-1922
Formulation of therapeutic proteins into particulate forms is a main strategy for site‐specific and prolonged protein delivery as well as for protection against degradation. Precise control over protein particle size, dispersity, purity, as well as mild preparation conditions and minimal processing steps are highly desirable. It is, however, hard to fit all these criteria with conventional preparation techniques. Here a one‐step hard‐templating synthesis of microparticles composed of functional, non‐denatured protein is reported. The method is based on filling porous CaCO3 microtemplates with the protein near to its isoelectric point (pI) followed by pH‐ or EDTA‐mediated dissolution of the tempplates. In principle, a wide variety of proteins can be converted into microparticles using this approach. The main requirement is an overlap of the protein insolubility and a template solubility for a certain parameter (here pH or EDTA). Here the formulation of insulin particles is studied in detail and it is shown that particles consisting of high molecular weight protein (catalase) can also be prepared. In this context, the synthesis of CaCO3 templates with controlled size, the mechanism of the protein microparticle formation and mechanical properties of the microparticles are discussed. For the first time, the fabrication of mesoporous monodispersed CaCO3 microtemplates with identical porocity but tuned diameter from 3 to 20 μm is demonstrated. The protein particle diameter can be adjusted by choosing the appropriate template size that is critical for successful pulmonary delivery of insulin. As a first step towards insulin delivery, the in vitro release of insulin at physiological conditions is studied. 相似文献
92.
L.S.L. Fernandes F.C. Moraes Filho J.B.A. Paulo J.A. Oliveira 《Control Engineering Practice》2013,21(8):1121-1127
The mixer-settler based on phase inversion (MSBPI) is a system used for treatment of wastewaters in order to reduce the content of oils and greases from wastewaters produced in oil industry. Its operation principle combines mixing, extraction and separation by decantation in a unique process. In particular, for control purpose, the level of the interface between organic solvent and water, in the separation section, is a critical variable to guarantee performance and security during operation of the MSBPI. In this work, an image-based detector to monitor the organic solvent–water interface level is proposed and a feedback control loop for the process is presented. Initially, a conventional controller (PID) was implemented and, subsequently, a strategy of gain scheduling adaptive control was developed in order to improve the process response in closed-loop. The experimental results showed that the adaptive control presented an efficient performance, even when disturbances of significant magnitude were applied to the process. 相似文献
93.
Damien Trentesaux Cyrille Pach Abdelghani Bekrar Yves Sallez Thierry Berger Thérèse Bonte Paulo Leitão José Barbosa 《Control Engineering Practice》2013,21(9):1204-1225
Benchmarking is comparing the output of different systems for a given set of input data in order to improve the system’s performance. Faced with the lack of realistic and operational benchmarks that can be used for testing optimization methods and control systems in flexible systems, this paper proposes a benchmark system based on a real production cell. A three-step method is presented: data preparation, experimentation, and reporting. This benchmark allows the evaluation of static optimization performances using traditional operation research tools and the evaluation of control system's robustness faced with unexpected events. 相似文献
94.
Tactic planning or master production scheduling focuses on time and spatial decomposition of the aggregate planning targets and forecasts, as well as, forecast and provision of needed resources. This process becomes extremely hard and time consuming with the increase of number of products, resources and periods considered. In face of such obstacles, this work shows a study of an Artificial Intelligence technique called Simulated Annealing applied to the optimization of production planning problem, more specifically, Master Production Scheduling. This work reviews some of the fundamental theory of simulated annealing, the methodology for master production scheduling calculation, the applicability of simulating annealing to planning problems, most important results and suggestions for further studies. 相似文献
95.
96.
We report the results of an empirical study demonstrating the value of using physiological compliance as a measure of social presence during digital game playing. The physiological activity (facial EMG, electrodermal activity, cardiac activity and respiration) of 21 dyads were acquired synchronously while they were playing a digital game either cooperatively or competitively and either at home or in the laboratory. Physiological compliance was defined as the correlation between the physiological signals of the dyad members. The results of this study confirm that physiological compliance is higher in a conflicting situation than when playing cooperatively. Importantly, the results also demonstrate that physiological compliance is related to self-reported social presence. This suggests that physiological compliance is not limited to negative situations but rather increases due to rich interactions. Only minor differences in physiological compliance were observed between home play and laboratory play, suggesting the ecological validity of laboratory measures. Finally, we propose that compliance measures can be considered as objective indices of social presence in digital gaming. 相似文献
97.
Cybele Lotti Rogério M.B. Moreno Paulo de S. Gonçalves Satinath Bhattacharya Luiz H.C. Mattoso 《Polymer Engineering and Science》2012,52(1):139-148
Natural rubber (NR) is a biopolymer whose properties depend on the molecular structure of the 1,4‐cis polyisoprene chains, nonrubber constituents, environmental conditions, etc. NR has been characterized by Mooney viscosity, Wallace plasticity, nitrogen content (%N). However, these cannot effectively account for clone's differences. The aim of this work is to use extensional rheology to characterize and differentiate NR samples as for clone type and the season of the year in comparison to the traditional characterizations. Three IAC 300 series and RRIM 600 clones of Hevea brasiliensis tapped between October 2006 (Oct_06) and August 2008 (Aug_08) were investigated. IAC 329 clones showed the least susceptibility to seasonal changes, whereas RRIM 600 was the most influenced. An opposite trend between extensional viscosity (ηE) and %N was established. The former was very sensitive to changes in the molecular structure of NR, being fundamental for monitoring purposes and strategic development of new rubber tree clones. POLYM. ENG. SCI., 2012. © 2011 Society of Plastics Engineers 相似文献
98.
Valério Rosset Pedro F. Souto Paulo Portugal Francisco Vasques 《Computer Standards & Interfaces》2012,34(3):281-291
We present reliability models for a group membership protocol designed for TDMA networks such as FlexRay, a protocol that is likely to become the de facto standard for next generation automotive networks. The models are based on discrete-time Markov chains and consider a comprehensive set of fault scenarios. Furthermore, they are parametric allowing for a sensitivity analysis. The results, obtained by a numeric solution of the models using the PRISM model-checker, show that they are computationally practical for realistic configurations and that the GMP can achieve reliability levels in the range required for safety critical applications. 相似文献
99.
Paulo H. R. Borges Neil B. Milestone Juliana O. Costa Cyril J. Lynsdale Túlio H. Panzera André L. Christophoro 《Materials and Structures》2012,45(5):663-678
Blended cement pastes are currently used for encapsulation of low level and intermediate level nuclear waste in the UK. However,
there is still little information on the long-term durability of those mixes to some chemical attacks. Accelerated testing
may predict the long-term durability or at least help the selection of more durable formulations. In this work, blended blastfurnace
slag (BFS)/Portland cement (OPC) pastes containing 60, 75 and 90% BFS and pulverised fuel ash (PFA)/OPC pastes with 40, 55
and 75% PFA were cured at 20 and 60°C for 90 days then submitted to natural and accelerated carbonation (5% CO2). The effects of the curing temperature as well as the OPC replacement level on the carbonation ratio are presented. Results
showed a good correlation between natural and accelerated carbonation for the pastes studied. Carbonation was found to be
governed by the amount of calcium hydroxide available in the mixes before the process started. 相似文献
100.
Paulo A.N. Dias José A.C. Alves Duncan Paul Fagg Marco S. Reis Maria H. Gil 《乙烯基与添加剂工艺杂志》2012,18(2):95-104
A new material has been developed consisting of pieces of wood embedded within a matrix of acrylic polymer, resulting in a transparent or semitransparent wood‐based product. This material presents quite appealing aesthetic features, thereby opening new possibilities for decorative applications. Because acrylic and methacrylic monomers are in the liquid state at room temperature, it is possible to introduce wood (in the current case, walnut wood) into a mixture of acrylic (hydroxypropyl acrylate) and/or methacrylic monomers (methyl methacrylate and 2‐hydroxyethyl methacrylate) along with a plasticizer (dioctyl phthalate) in the presence of a chemical initiator (benzoyl peroxide). A transparent polymeric matrix with dispersed wood is then obtained through bulk free‐radical polymerization. Introducing this reaction mixture along with pieces of wood into a mold results in a wood‐polymer composite. A 24?1 experimental fractional factorial design was implemented to study the importance of the composition of these materials on several relevant properties. The sheets produced were characterized by tensile testing, dynamic mechanical thermal analysis, thermal gravimetric analysis, and heat deflection temperature. The models obtained for predicting each property pointed to valuable insights regarding the influential constituents. In particular, our results suggested that monomers to be used in future applications of this material should be selected in terms of their cost and the desired flexibility for the final product, not in terms of their polarity. J. VINYL ADDIT. TECHNOL., 2012. © 2012 Society of Plastics Engineers 相似文献