Comparison of Three Flexural Retrofit Systems under Monotonic and Fatigue Loads

The majority of experimental work involving the flexural retrofit of concrete bridge girders has been conducted on beam specimens with adhesive-applied, soffit-mounted, fiber-reinforced polymer (FRP) composite systems, referred to in this study as conventional adhesive application (CAA). It has been observed that the performance of such girders is often controlled by the quality of the bond between the FRP and the concrete substrate and the substrate’s ability to transfer stress from the steel to the FRP. With the goal of improving the performance of bonded FRP in mind, two additional soffit-mounted retrofit schemes are investigated: near-surface mounted (NSM), where the FRP strips are embedded in adhesive within slots cut into the substrate concrete, and, powder-actuated fastener-applied (PAF) FRP, which uses a powder-actuated nail gun to install mechanical fasteners through predrilled holes in the FRP into the concrete substrate, “nailing” the FRP in place. The PAF application is a recent development, and little work has been done on it other than by the proprietors of the system. This study reports on a comparative study of the static and fatigue performance of reinforced concrete beams retrofitted with CAA, NSM, and PAF FRP retrofit systems. Ten medium-scale beams were tested: six strengthened specimens, two per retrofit method, were tested under cyclic loading conditions, and four specimens, one per retrofit method and one control specimen, were tested monotonically to failure. The results of this study indicate that although all three methods of FRP application result in significant strength increases over the control specimen under monotonic loading conditions, the CAA method is outperformed by the other methods under cyclic conditions. A number of other relevant detailed conclusions with respect to performance and practical application issues are presented for each of the methods of retrofit examined in this study. Significantly, clear evidence of FRP debonding in the midspan region prior to specimen failure is presented.     

Glycerin suppression of fluorescence self-quenching and improvement of heterogeneous fluoroimmunoassay sensitivity

Fluorescent labels find wide application in immunoassays and immunosensors as well as in protein and DNA chips. However, the use of fluorescent labels in applications requiring high detection sensitivity is limited by fluorescence self-quenching observed when a relatively high number of fluorescent compounds is introduced in the recognition molecule. Here we describe a simple method that suppresses effectively fluorescence self-quenching observed when highly labeled antibodies are used as labels in immunoassays. This was achieved by treating the microtitration wells after the completion of the immunoassay with a glycerin solution followed by 15-min incubation of the emptied wells at 37 degrees C. The remedial action of this method on self-quenching was studied through a noncompetitive immunofluorometric assay for rabbit gamma-globulins employing a sheep anti-rabbit gamma-globulin antibody labeled with fluorescein at molar ratios ranging from 1.0 to 17.4. The glycerin/thermal treatment increased the fluorescence signal measured directly onto the solid surface by 9.2-117% for the antibodies with molar ratios of 1.0-17.4, compared with the values obtained prior to treatment. Furthermore, fluorescence self-quenching was completely removed for labeling ratios up to 14.0. The assay sensitivity was improved 2-4 times by the glycerin/thermal treatment when heavily fluoresceinated antibodies are used as labels (molar ratio >/=5.6). The proposed method resulted also in increased fluorescence signals when labels other than fluorescein were used and improved considerably the detection of protein spots on silicon dies.     

Region-based remote sensing image compression in wavelet domain using free angle segmentation model

A novel compression algorithm suitable for remote sensing applications is proposed. Exploiting the high density of edge points, textured regions are identified and removed from the encoding process. The rest of the image is encoded in the wavelet domain. Experimental results show significant improvements compared to standard coding approaches.     

Sequential recogniser combination

A Korean character recogniser which performs better than a commercially available system, and a methodology for accelerating its performance, combining it with some other fast and non-reliable recognisers, is proposed.