Effects of Lactobacillus casei and Alterations in Fermentation Conditions on Biosynthesis of Glucuronic Acid by a Dekkera bruxellensis-Gluconacetobacter intermedius Kombucha Symbiosis Model System

A combination of Dekkera bruxellensis and Gluconacetobacter intermedius is used as a model system to emulate the microbial symbiosis between yeast and acetic acid bacteria in kombucha, and to study glucuronic acid (GlcUA) production in this system. In the current study, the addition of Lactobacillus casei and variations in the fermentative conditions, such as sucrose concentration, temperature and duration, were examined to evaluate their effects on GlcUA formation. Results showed that L. casei acts as a supporter species and stimulated GlcUA production in the kombucha symbiosis model to levels 54.1% greater than in the control. Varying the fermentation parameters also affected GlcUA production. Therefore, the range of each factor value was screened and restricted to achieve a higher amount of target acid, as it was 5–15%, 25–31°C, 3–7 days, and 5–7 units in sucrose concentration, temperature, fermentation time and initial pH value, respectively. These findings are an important step for optimizing GlcUA production by this model system.     

Biological,Chemical, and Electronic Applications of Nanofibers

With their high‐surface‐to‐volume ratio, nanofibers have been postulated to increase interactions between nanofibrous materials and targeted substrates, which are helpful to overcome many obstacles and enhance the efficiency in a diverse number of applications. Over the past decade, many studies have been published on the fabrication of nanofibers and their applications in various fields. In this review, novel biological, chemical, and electrical characteristics of nanofibers as well as their recent status and achievements in medicine, chemistry, and electronics are analyzed. It is found that nanofibers can induce fast regeneration of many tissues/organs in medical applications and improve the efficiency of many chemical and electronics applications.

     

Employee attitude surveys: Examining the attitudes of noncompliant employees.

Employees (N? = ?194) from a wide variety of organizations participated in this study aimed at describing the attitudes of individuals who refuse to respond to an employee survey request (noncompliants). Noncompliants, in comparison with those individuals who would comply with the survey request, possessed greater intentions to quit, less organizational commitment, and less satisfaction toward supervisors and their own jobs. Noncompliants also possessed more negative beliefs regarding how their organization handles employee survey data (e.g., does not act on survey data). No significant differences were found for work-related demographic variables, satisfaction with pay, and satisfaction with promotion opportunities. Implications for survey research are discussed along with methods to address nonresponse and noncompliance. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Do Agile Managed Information Systems Projects Fail Due to a Lack of Emotional Intelligence?

Information Systems Frontiers - Agile development methodologies (ADM) have become a widely implemented project management approach in Information Systems (IS). Yet, along with its growing...     

Monolithic, multiple-wavelength vertical-cavity surface-emitting laser arrays by surface-controlled MOCVD growth rate enhancement and reduction

Monolithic, multiple-wavelength vertical-cavity surface-emitting laser (VCSEL) arrays have been obtained by the surface-controlled enhancement and reduction of the MOCVD epitaxial growth rate, achieving a periodic, graded wavelength span greater than 30 nm. Room-temperature (RT), electrically pumped continuous-wave (CW) lasing is demonstrated, with uniform threshold currents of 5.5/spl plusmn/0.5 mA with typical output powers of 0.5 mW. We show here for the first time both the enhancement and the reduction of the growth rate of the entire VCSEL structure and demonstrate the controlled variation of the VCSEL lasing wavelength over a widened spectral range by exploiting both effects.     

Energy harvesting assisted cognitive radio: random location-based transceivers scheme and performance analysis

We consider spectrum-sharing scenario where coexist two communication networks including primary network and secondary network using the same spectrum. While the primary network includes directional multi-transceivers, the secondary network consists of relaying-based transceiver forwarding signals by energy harvesting assisted relay node. In cognitive radio, signals transmitted from secondary network are sufficiently small so that all of primary network receivers have signal to noise ratio (SNR) greater than a given threshold. In contrast, the transmitted signals from primary network cause increasing noise which is difficult to demodulate at secondary network nodes and hence it leads to the peak power constraint. In this paper, we focus on the influence of random location of transceivers at primary network using decode-and-forward protocol. Specifically, we derive closed-form outage probability expression of the secondary network under random location of transceivers and peak power constraint of primary network. This investigation shows the relationship between the fraction of energy harvesting time \(\alpha \) of time switching-based relaying protocol on outage probability of secondary network and throughput. In addition, we analyse the influence of the number of primary network transceivers as well as primary network’s SNR threshold on secondary network. Furthermore, the trade-off between increasing energy harvesting and rate was investigated under the effect of energy conversion efficiency. The accuracy of the expressions is validated via Monte-Carlo simulations. Numerical results highlight the trade-offs associated with the various energy harvesting time allocations as a function of outage performance.     

Self-Calibration of a Moving Camera from Point Correspondences and Fundamental Matrices

We address the problem of estimating three-dimensional motion, and structure from motion with an uncalibrated moving camera. We show that point correspondences between three images, and the fundamental matrices computed from these point correspondences, are sufficient to recover the internal orientation of the camera (its calibration), the motion parameters, and to compute coherent perspective projection matrices which enable us to reconstruct 3-D structure up to a similarity. In contrast with other methods, no calibration object with a known 3-D shape is needed, and no limitations are put upon the unknown motions to be performed or the parameters to be recovered, as long as they define a projective camera.The theory of the method, which is based on the constraint that the observed points are part of a static scene, thus allowing us to link the intrinsic parameters and the fundamental matrix via the absolute conic, is first detailed. Several algorithms are then presented, and their performances compared by means of extensive simulations and illustrated by several experiments with real images.     

Separation and determination of polycyclic aromatic hydrocarbons by solid phase microextraction/cyclodextrin-modified capillary electrophoresis

A sensitive method for the determination of polycyclic aromatic hydrocarbons (PAHs) by solid phase microextraction coupled with cyclodextrin (CD)-modified capillary electrophoresis (CE) using UV detection has been developed. A glass fiber was prepared and used for absorbing 16 EPA priority PAHs from diluted samples until equilibrium was reached. After the glass fiber was connected to a separation capillary via an adapter, the absorbed analytes were directly released into the CE buffer stream, and electrophoretic separation was effected using a 50 mM borate, pH 9.2, buffer containing 35 mM sulfobutyloxy-β-CD, 10 mM methyl-β-CD, and 4 mM α-CD. Separation was effected since neutral PAHs differentially partitioned between the neutral and charged CD phases. Under 30 kV applied potential, separation was achieved in less than 15 min with high resolution and number of theoretical plates. Pyrene as low as 8 ppb was detected, while the highest limit of detection was 75 ppb for acenaphthene. Very satisfactory reproducibility with respect to migration time and peak area was obtained for repetitions using the same separation capillary and adapter, where only the extraction fiber was discarded after each analysis.