Pesticide monitoring programme of the Ministry of Health of Brazil

The structure of the recently introduced Brazilian government programme for monitoring pesticide residues in six major agricultural products is described. Residues found from the analysis of samples of tomatoes and strawberries are discussed in detail. The much greater frequency of detection of pesticide residues, many of which are not approved for use in Brazil, in produce being sold on the domestic market, as opposed to that produced for export, is of major concern. It is perceived that this new monitoring programme will become permanent and be used to underpin enforcement actions that will need to be taken to reduce the numbers of incidents of illegal residues occurring, particularly in fresh fruits and vegetables.     

The VLISP verified PreScheme compiler

This paper describes a verified compiler for PreScheme, the implementation language for thevlisp run-time system. The compiler and proof were divided into three parts: A transformational front end that translates source text into a core language, a syntax-directed compiler that translates the core language into a combinator-based tree-manipulation language, and a linearizer that translates combinator code into code for an abstract stored-program machine with linear memory for both data and code. This factorization enabled different proof techniques to be used for the different phases of the compiler, and also allowed the generation of good code. Finally, the whole process was made possible by carefully defining the semantics ofvlisp PreScheme rather than just adopting Scheme's. We believe that the architecture of the compiler and its correctness proof can easily be applied to compilers for languages other than PreScheme.This work was supported by Rome Laboratory of the United States Air Force, contract No. F19628-89-C-0001, through the MITRE Corporation, and by NSF and DARPA under NSF grants CCR-9002253 and CCR-9014603. Author's current address: Department of Computer Science and Engineering, Oregon Graduate Institute, P.O. Box 91000, Portland, OR 97291-1000.The work reported here was supported by Rome Laboratory of the United States Air Force, contract No. F19628-89-C-0001. Preparation of this paper was generously supported by The MITRE Corporation.This work was supported by Rome Laboratory of the United States Air Force, contract No. F19628-89-C-0001, through the MITRE Corporation, and by NSF and DARPA under NSF grants CCR-9002253 and CCR-9014603.     

Binding Properties of RNA Quadruplex of SARS-CoV-2 to Berberine Compared to Telomeric DNA Quadruplex

Previous studies suggest that berberine, an isoquinoline alkaloid, has antiviral potential and is a possible therapeutic candidate against SARS-CoV-2. The molecular underpinnings of its action are still unknown. Potential targets include quadruplexes (G4Q) in the viral genome as they play a key role in modulating the biological activity of viruses. While several DNA-G4Q structures and their binding properties have been elucidated, RNA-G4Qs such as RG-1 of the N-gene of SARS-CoV-2 are less explored. Using biophysical techniques, the berberine binding thermodynamics and the associated conformational and hydration changes of RG-1 could be characterized and compared with human telomeric DNA-G4Q 22AG. Berberine can interact with both quadruplexes. Substantial changes were observed in the interaction of berberine with 22AG and RG-1, which adopt different topologies that can also change upon ligand binding. The strength of interaction and the thermodynamic signatures were found to dependent not only on the initial conformation of the quadruplex, but also on the type of salt present in solution. Since berberine has shown promise as a G-quadruplex stabilizer that can modulate viral gene expression, this study may also contribute to the development of optimized ligands that can discriminate between binding to DNA and RNA G-quadruplexes.     

Neuropathic Pain Induces Interleukin-1β Sensitive Bimodal Glycinergic Activity in the Central Amygdala

Neuropathic pain reduces GABA and glycine receptor (GlyR)-mediated activity in spinal and supraspinal regions associated with pain processing. Interleukin-1β (IL-1β) alters Central Amygdala (CeA) excitability by reducing glycinergic inhibition in a mechanism that involves the auxiliary β-subunit of GlyR (βGlyR), which is highly expressed in this region. However, GlyR activity and its modulation by IL-1β in supraspinal brain regions under neuropathic pain have not been studied. We performed chronic constriction injury (CCI) of the sciatic nerve in male Sprague Dawley rats, a procedure that induces hind paw plantar hyperalgesia and neuropathic pain. Ten days later, the rats were euthanized, and their brains were sliced. Glycinergic spontaneous inhibitory currents (sIPSCs) were recorded in the CeA slices. The sIPSCs from CeA neurons of CCI animals show a bimodal amplitude distribution, different from the normal distribution in Sham animals, with small and large amplitudes of similar decay constants. The perfusion of IL-1β (10 ng/mL) in these slices reduced the amplitudes within the first five minutes, with a pronounced effect on the largest amplitudes. Our data support a possible role for CeA GlyRs in pain processing and in the neuroimmune modulation of pain perception.     

PMD and PDL monitoring of traffic-carrying transatlantic fibre-optic system

For the first time the direct monitoring of polarisation mode dispersion (PMD) and polarisation-dependent loss (PDL) in a traffic-carrying transatlantic fibre-optic system without the requirement of loopingback is reported. The impact of transmitter polarisation scrambling in the measured results is analysed and verified by the measured results.     

WSN Deployment and Localization Using a Mobile Agent

To address the problem the sensors were typically deployed in fixed positions, but the robots can be used to calibrate, deploy and maintain the surrounding wireless sensor network (WSN) in disaster relief applications, a novel framework was proposed to obtain a wide coverage of the unknown environment by the sensors, which can help the robot during the disaster recovery activities, for the concurrent deployment and localization of a WSN by means of a mobile robot. During the mission, the robot explored an unknown environment, and was equipped with both proprioceptive sensors, range finders and wireless antennas. Moreover, the robot carried a set of nodes, and it can deploy them while exploring the unknown environment. Variou experimental results showd the proposed algorithm can outperform trilateration method in unknown environment exploration and network coverage problems.     

Digital control of a voltage-mode synchronous buck converter

A digital control algorithm capable of separately specifying the desired output voltage and transient response for a synchronous buck converter operating in voltage mode was developed. This algorithm is based on superimposing a small control signal onto a voltage reference at each switching cycle to cancel out the perturbations. A zero steady-state error in the output voltage can be obtained with the aid of additional dynamics to allow the controller to track a load change and update the reference to a new load state. The specifications of the control algorithm are achieved by pole placement using complete state feedback. The control algorithm was implemented on a digital signal processor (DSP)-controlled synchronous buck converter.     

Susceptibility of Listeria monocytogenes isolated from food in Italy to antibiotics

The susceptibility of 148 strains of Listeria monocytogenes isolated from food to antibiotics currently used in veterinary and human therapy was determined by standard agar dilution and disk diffusion methods. The antibiotics included amikacin, amoxicillin, cefazolin, chloramphenicol, erythromycin, flumequine, fosfomycin, gentamicin, kanamycin, lincomycin, oxytetracycline, rifampicin, spiramycin, streptomycin, tetracycline, tobramycin and vancomycin. Soussy's breakpoints and MIC(50)-MIC(90) values were used to classify the strains into sensitive, moderately sensitive and resistant groups.This work is part of a wider surveillance program on listeriosis started in Italy in 1995.     

Time course of vinblastine-induced autophagocytosis and changes in the endoplasmic reticulum in murine pancreatic acinar cells: a morphometric and biochemical study

The time course of the vinblastine(-sulfate; 10 mg/kg body weight, single injection)-induced enlargement and subsequent regression of the autolysosomal compartment was studied by electron microscopic morphometrical and cell biochemical methods in order to gain information concerning some key problems of this major route of intralysosomal degradation of the cell's endogenous macromolecules and structures. Detailed analysis of the dynamics of the total autophagic vacuole (AV) compartment and its different subcompartments (early, advanced, late, and fused AVs), as well as of changes of rough-surfaced endoplasmic reticulum (RER) showed: 1. Pancreatic acinar cells react to vinblastine biphasically, i.e. two expansion phases of the AV compartment, the first in the 0 to 90 min and the second in the 2 to 8 h post-injectional periods, were detected. 2. Fusions of AVs are not inhibited by vinblastine, at least during the second expansion phase when cytoplasmic volume fraction (CVF) of fused AVs steadily increased until the 12th h. Fusion of early, advanced and late AVs or composition of fused complex vacuoles (AVc) are somehow regulated, as the proportion of the three AV stages from the CVF of AVc, was maintained constant throughout the second expansion phase. 3. Stimulation of autophagosome formation and resulting substrate overload seems to be the primary mode of action by which vinblastine causes the enormous expansion of the autolysosomal compartment. 4. Degranulation of the rough-surfaced endoplasmic reticulum (RER) membranes occurs in a biphasic fashion, similarly to the volume and surface changes of the AV compartment, thus supporting our previous hypothesis, that labilization or change of RER may have a role in the formation of autophagosomes. 5. Vinblastine-induced autophagocytosis is a selective process, as mitochondria, Golgi elements and zymogen granules are very much underrepresented, whereas RER is more than twice overrepresented in the volume of early AVs, when compared to their volume fraction in the whole cytoplasm. 6. Immunogold electron microscopy revealed the presence of ubiquitinylated proteins in advanced and late, but not in early AVs.