Filtering out contrast reversals for microscopy autofocus

Autofocus functions based on measurement of image resolution appear to be precise and robust for biological microscopy. However, the through-focus response of these functions previously exhibited unwanted local maxima, or side peaks. Here we report theoretical and experimental studies showing that side peaks are mainly a result of contrast reversals inherent in optical systems at mid-range frequencies. These contrast reversals are not present in frequencies near optical cutoff. Contrast reversals thus limit the lower cutoff for resolution measurement filters, whereas signal-to-noise limits the upper cutoff. These improved bandpass design criteria led to sharp, unimodal autofocus responses for all tested microscopy specimens.     

Biologically Inspired,Cell‐Selective Release of Aptamer‐Trapped Growth Factors by Traction Forces

Biomaterial scaffolds that are designed to incorporate dynamic, spatiotemporal information have the potential to interface with cells and tissues to direct behavior. Here, a bioinspired, programmable nanotechnology‐based platform is described that harnesses cellular traction forces to activate growth factors, eliminating the need for exogenous triggers (e.g., light), spatially diffuse triggers (e.g., enzymes, pH changes), or passive activation (e.g., hydrolysis). Flexible aptamer technology is used to create modular, synthetic mimics of the Large Latent Complex that restrains transforming growth factor‐β1 (TGF‐β1). This flexible nanotechnology‐based approach is shown here to work with both platelet‐derived growth factor‐BB (PDGF‐BB) and vascular endothelial growth factor (VEGF‐165), integrate with glass coverslips, polyacrylamide gels, and collagen scaffolds, enable activation by various cells (e.g., primary human dermal fibroblasts, HMEC‐1 endothelial cells), and unlock fundamentally new capabilities such as selective activation of growth factors by differing cell types (e.g., activation by smooth muscle cells but not fibroblasts) within clinically relevant collagen sponges.     

The influence of the Amazonian floodplain ecosystems on the trace element dynamics of the Amazon River mainstem (Brazil)

The purpose of this paper is to forecast the role of riverine wetlands in the transfer of trace elements. One of the largest riverine wetlands in the world is the floodplain (várzea) of the Amazon River and its tributaries (Junk and Piedade, 1997). The central Amazon wetlands are constituted by a complex network of lakes and floodplains, named várzeas, that extend over more than 300,000 km2 (Junk, W.J., The Amazon floodplain--a sink or source for organic carbon? In Transport of Carbon and Minerals in Major World Rivers, edited by E.T. Degens, S. Kempe, R. Herrera, SCOPE/UNEP; 267-283, 1985.) and are among the most productive ecosystems in the world due to the regular enrichment in nutrients by river waters In order to understand if the adjacent floodplain of Amazon River have a significant influence on the trace element concentrations and fluxes of the mainstem, the concentrations of selected elements (i.e., Al, Mn, Fe, Co, Cu, Mo, Rb, Sr, Ba, and U) have been measured in the Amazon River water (Manacapuru Station, Amazonas State, Brazil) and in lake waters and plants (leaves) from a várzea(Ilha de Marchantaria, Amazonas State, Brazil) during different periods of the hydrological cycle. Four plant species (two perennial species: Pseudobombax munguba and Salix humboldtiana, and two annual herbaceous plants: Echinochloa polystachya and Eichhornia crassipes) were selected to represent the ecological functioning of the site. Time series obtained for dissolved Mn and Cu (<0.20 microm) in Amazon River water could not be explained by tributary mixing or instream processes only. Therefore, the contribution of the waters transiting the floodplains should be considered. These results suggest that the chemical composition of the waters draining these floodplains is controlled by reactions occurring at sediment-water and plant-water interfaces. Trace elements concentrations in the plants (leaves) vary strongly with hydrological seasonality. Based on the concentration data and the biological productivity of floodplain ecosystems, a first order approximation of trace element storage (permanent or temporary) in the vegetation of these floodplains was made. It was found that floodplain-mainstem elemental fluxes make a significant contribution to the dissolved flux of the Amazon River. This study is part of the Brazilian_French joint research program Hybam (Hydrology and Geochemistry of the Amazonian Basin).     

Numerical simulation of dehumidifying fin-and-tube heat exchangers: Semi-analytical modelling and experimental comparison

The aim of this paper is to present a developed semi-analytical model for the simulation of dehumidifying air–liquid fin-and-tube heat exchangers. The simulation strategy and the mathematical methodology are described in detail. The model is based on -NTU method, and formulated in a compact way for dry and wet surface situations (temperature or enthalpy driven, respectively). Both rating and design procedures have been developed for fully dry, partially wet, or fully wet surface conditions. The model predictions are compared with experimental data obtained on a wavy and a plain finned heat exchanger, giving reasonably accurate results. The limitations of the empirical information used are clearly identified in the work. The aim of this model is to provide a fast but reliable rating and design numerical tool for air–liquid heat exchanger applications.     

Bioremediation of 2,4,6-trinitrotoluene under field conditions

In situ bioremediation of the nitroaromatic explosive 2,4,6-trinitrotoluene (TNT) provides a cost-effective alternative for cleaning up contaminated sites. Here we compare the effectiveness of several bioremediation techniques: natural attenuation, bioaugmentation with TNT-degrading Pseudomonas putida JLR11, phytoremediation with maize (Zea mays L.) and broad beans (Vicia faba L.), and rhizoremediation with maize and broad beans inoculated with P. putida JLR11. Experiments in spiked hydroponic medium demonstrated that inoculation with bacteria did not affect TNT levels. On the other hand, axenic plants were able to remove 32% to 38% of the TNT from the medium. However, when plants were inoculated with bacteria,TNT disappeared to an even greater extent (80% to 88%), a result that advocates a role for P. putida JLR11 in rhizoremediation. In field experiments neither natural attenuation nor bioaugmentation with P. putida JLR11 affected TNT levels to a significant degree. However, the extractable TNT content in rhizosphere soil associated to maize roots decreased by more than 96% in 60 days regardless of inoculation. This indicates that under these field conditions, the effect of phytoremediation by maize overshadowed any effect of rhizoremediation by P. putida JLR11.     

A Double Energy Transition of Nanocrystalline CdxZn1-xS Films Deposited by Chemical Bath

Ternary films of Cd_xZn_1-xS were deposited on Corning glass substrates by the chemical bath deposition technique at 80°C. A process for depositing thin films of the ternary Cd_xZn_1-xS is proposed. Different induction times in the methodology for films deposition were included. The chemical bath was composed of zinc chloride, thiourea, and cadmium chloride as source of the main ions, ammonium nitrate as complexing agent, and potassium hydroxide for pH control. Groups of samples with different conditions were deposited to obtain the ternary material. The manufacturing processes as well as the chemical reactions that take place during preparation are discussed. The morphology, the crystalline structure, and the optical properties of the deposited films were obtained. X-ray diffraction results revealed hexagonal structures of the Cd_xZn_1-xS material in all the deposited samples and structures of ZnO in some cases. Energy dispersive spectroscopy results show the presence of Cd, Zn, and S as main components of the material. A double transition on the absorption measurements were obtained on the produced ternary films which is associated with a double bandgap energy.     

Three-dimensional numerical simulation of convection and radiation in a differentially heated cavity using the discrete ordinates method

The main purpose of this paper is to study, in a three-dimensional, differentially heated cavity, the phenomenon of radiation and natural convection in both transparent and participating media. The discrete ordinates method (DOM) is used to solve the radiative transfer equation. The Navier-Stokes equations (NSE), describing natural convection, are solved with a segregated SIMPLE-like algorithm. For non-participating media, the coupling between the radiative transfer and NSE is done via the radiative heat exchange between surfaces. For participating media, a source term is added in the energy equation. The local and mean heat flux as a function of the Rayleigh number is studied, for both transparent and participating media with different optical thicknesses. The effect of the Planck number on the heat flux is also analyzed for different values of the Rayleigh number. Also, a comparison between a purely two-dimensional case and the results obtained in the mid-plane of a long rectangular enclosure is presented.     

Canonical visual size for real-world objects.

Real-world objects can be viewed at a range of distances and thus can be experienced at a range of visual angles within the visual field. Given the large amount of visual size variation possible when observing objects, we examined how internal object representations represent visual size information. In a series of experiments which required observers to access existing object knowledge, we observed that real-world objects have a consistent visual size at which they are drawn, imagined, and preferentially viewed. Importantly, this visual size is proportional to the logarithm of the assumed size of the object in the world, and is best characterized not as a fixed visual angle, but by the ratio of the object and the frame of space around it. Akin to the previous literature on canonical perspective, we term this consistent visual size information the canonical visual size. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Cross-Batch Contamination in a Continuous Horizontal Decanter Centrifuge during Virgin Olive Oil Production

Cross-batch contamination in a decanter centrifuge during virgin olive oil production cannot be avoided using current technology. The extent of this contamination is investigated using industrial-scale tests, by measuring the volatile profile and color on three consecutive oil batches, collected at the decanter outlet at different extraction times. The extent of contamination varied, pointing out qualitative consequences, as defective molecules are found. The latter are often active at low concentrations, and the measured cross-batch contamination can lead both to the downgrading of large batches of virgin olive oils and to the adulteration of monovarietal and certified productions. An innovative method, based on the direct determination of the color (L and a* coordinates) of oil at the outlet of the decanter is able to identify the same compositional change point indicated by gas chromatography, and could be successfully used to mitigate the effects of cross-batch contamination. Practical applications: An in-line colorimetric system can be implemented at the decanter outlet to detect the point of change between different olive batches. Otherwise, the virgin olive oil exiting from the decanter at the beginning of one batch can be collected separately in order to avoid the contamination due to the previous batch.