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41.
KA Birkness M Deslauriers JH Bartlett EH White CH King FD Quinn 《Canadian Metallurgical Quarterly》1999,67(2):653-658
A tissue culture bilayer system that mimics some aspects of early alveolar infection by Mycobacterium tuberculosis was developed. This model incorporates human lung epithelial type II pneumocyte (A549) (upper chamber) and endothelial cell (lower chamber) layers separated by a microporous membrane. This construction makes it possible to observe and quantify the passage of bacteria through the two layers, to observe the interaction of the bacteria with the various cell types, and to examine the basic mechanisms of immune cell recruitment to the site of infection. After 10(7) organisms were added to the upper chamber we microscopically observed large numbers of bacteria attached to and within the pneumocytes and we determined by viable-cell counting that a small percentage of the inoculum (0.02 to 0.43%) passed through the bilayer into the lower chamber. When peripheral blood mononuclear cells were added to the lower chamber, microscopic examination indicated a migration of the mononuclear cells through the bilayer to the apical surface, where they were seen associated with the mycobacteria on the pneumocytes. The added complexity of the bilayer system offers an opportunity to define more precisely the roles of the various lung cell types in the pathogenesis of early tuberculosis. 相似文献
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Breen G. Collier D. Craig I. Quinn J. 《IEEE engineering in medicine and biology magazine》2008,27(2):103-108
The explosion of genetic data availability in the last decade has opened up many new avenues for the application of genetics to the improvement of human health, particularly, the common and complex disorders, which previously largely defied researchers seeking to understand their etiology. However, the utilization of this data in healthcare and by the pharmaceutical industry is still in its infancy, with the current focus being almost solely on two forms of genetic variation single nucleotide polymorphisms (SNPs), involving the substitution of one base in DNA for another and copy number variations, involving the deletion and multiplication of regions > 1,000 base pairs (bp). However, there is currently a relative paucity of funding and general interest in a third form of variation called microsatellites and mini- satellites. 相似文献
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Young infants show unexplained asymmetries in the exclusivity of categories formed on the basis of visually presented stimuli. A connectionist model is described that shows similar exclusivity asymmetries when categorizing the same stimuli presented to infants. The asymmetries can be explained in terms of an associative learning mechanism, distributed internal representations, and the statistics of the feature distributions in the stimuli. The model was used to explore the robustness of this asymmetry. The model predicts that the asymmetry will persist when a category is acquired in the presence of mixed category exemplars. An experiment with 3–4-month-olds showed that asymmetric exclusivity persisted in the presence of mixed-exemplar familiarization, thereby confirming the model's prediction. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
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J. M. Dulieu-Smith S. Quinn R. A. Shenoi P. J. C. L. Read S. S. J. Moy 《Applied Composite Materials》1997,4(5):283-303
A detailed study of the stresses that are developed in a glass reinforced plastic (GRP) tee joint under service loads is described.
The joints are fabricated by laminating a boundary angle over a radiused fillet on either side of the ‘tee’. Full-field stress
characterisation data is provided by a thermoelastic analysis of the tee joint. Calibration procedures that allow the thermoelastic
data to be compared with the results of a finite element analysis are detailed. The results of the thermoelastic analysis
are compared with values obtained from the finite element analysis. The applicability of thermoelastic analysis as a validation
tool for finite element models of composite materials is assessed. 相似文献
47.
Cricket-based robots 总被引:1,自引:0,他引:1
Birch M.C. Quinn R.D. Hahm G. Phillips S.M. Drennan B.T. Fife A.J. Beer R.D. Xinyu Yu Garverick S.L. Laksanacharoen S. Pollack A.J. Ritzmann R.E. 《Robotics & Automation Magazine, IEEE》2002,9(4):20-30
This article describes the development of an autonomous hybrid microrobot that uses legs for propulsion and support of the rear half of the body and a pair of wheels for support of the front half. McKibben artificial muscles actuate the legs, and compressed air is generated by an onboard power plant. Control is also onboard in the form of a PIC microcontroller, from Microchip Technology Inc., that controls the actuators through four three-way valves that are each made up of a pair of microelectromechanical system devices. Its motion resembles that of a cricket. 相似文献
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We present a new hydrodynamic modelling framework capable of fully exploiting modern graphics and central processing units (GPUs and CPUs) from any of the mainstream vendors, to be used in the design and assessment of sustainable drainage systems. A finite-volume Godunov-type scheme is combined with the HLLC Riemann solver to create a robust numerical model which correctly addresses wetting and drying and transient flow conditions, and is suitable for application to a wide range of flood simulations. The software is tested with a three day flood event in Carlisle during 2005, at resolutions from 25 m to 2 m. Run-times are significantly reduced without compromising numerical accuracy. Excellent agreement is found between the simulation results and a comprehensive post-event survey. Changes in sensitivity to Manning's n are examined at different resolutions, with changes to the floodplain found to have little influence at 2 m resolution. 相似文献