Great Lakes Beginning-of-Month Water Levels and Monthly Rates of Change of Storage

Time series of beginning-of-month water levels and rates of change of lake storage were determined for each of the Great Lakes and Lake St. Clair for 1941-1975 period. The Thiessen polygon procedure was used to compute the beginning-of-month levels because it provides more representative over-all lake levels than straight averaging and requires minimum subjectivity. The effect of crustal movement on the rate of change of lake storage was investigated and found to be negligible. A gage density analysis showed good agreement between various size gage networks with the maximum deviation between networks decreasing with increasing gage density. Thiessen polygon weighting factors are presented for the current gage networks to enable future extension of the time series.     

Relative Accuracy of Connecting Channel Discharge Data with Application to Great Lakes Studies

The flows in the Great Lakes connecting channels are a major component in the water balance of the Great Lakes Basin. The increased emphasis on Great Lakes water quality and quantity requires an assessment of the accuracy of both measured and computed connecting channel discharge data. In this study, the standard error of typical discharge measurements was found to be approximately 3 to 5 percent, depending upon the number of panels used in the cross section. Mesurement sets were found to have a practical limit of about 25 measurements. The standard error of a set of measurements was found to be on the order of 1 percent. The procedure used to compute the published flows of the Niagara River was found to have an apparent bias of about 2 percent on the high side. It is recommended that the published Niagara River flows be adjusted prior to use in detailed water balance studies.     

Molecular genetic analysis of plastocyanin biosynthesis in Chlamydomonas reinhardtii

Five plastocyanin-deficient mutants were identified from a population of UV-mutagenized Chlamydomonas reinhardtii cells. Genetic complementation experiments indicated that four mutants represented alleles at the PCY1 locus (pcy1-2, pcy1-3, pcy1-4, and pcy1-5). Sequence analysis confirmed that two strains, pcy1-2 and pcy1-3, carry a frameshift (-1) and a nonsense mutation, respectively, while strains pcy1-4 and pcy1-5 synthesize an extended protein as a result of read-through mutations at the stop codon. The C-terminal extension does not affect synthesis or processing of the pre-proteins, but the polypeptides are rapidly degraded after the second (lumenal) processing event. The frameshift mutation in pcy1-2 results in loss of Pcy1 mRNA, as noted previously for strain ac208 (pcy1-1), but the abundance of Pcy1 mRNA in strain pcy1-3, which carries a nonsense mutation at codon 26, is unaffected relative to wild-type cells. The decreased abundance of frameshifted Pcy1 mRNA is attributed to increased degradation rather than decreased synthesis, since the mRNAs can be stabilized by treatment of cells with cycloheximide or anisomycin. The fifth strain has a wild-type plastocyanin-encoding gene, but the strain accumulates apoplastocyanin at the expense of holoplastocyanin. We suggest that the mutation identifies a new locus (PCY2) whose function is required for normal holoplastocyanin accumulation. Like ac208 (pcy1-1), several of the new mutants were suppressed spontaneously owing to accumulation of cytochrome c6 (a functional substitute for plastocyanin). The suppressor mutation(s) displayed Mendelian inheritance and segregated independently from the PCY1 locus, which confirms that regulation of Cyc6 expression is not tightly linked to plastocyanin function.     

Effects of injurious compression on matrix turnover around individual cells in calf articular cartilage explants

The effects of mechanical injury on the metabolism of cartilage matrix are of interest for understanding the pathogenesis of osteoarthrosis and the development of strategies for cartilage repair. The purpose of the present study was to examine the effects of injury on matrix turnover in a calf articular cartilage explant system for which the effects of mechanical loading on cell activity and the cell-mediated pathways of matrix metabolism are already well characterized. New methods of quantitative autoradiography were used in combination with established biochemical and biomechanical techniques for the analysis of cell and matrix responses to acute mechanical injury, with particular attention to the processes of localized matrix turnover in the cell-associated matrices of individual chondrocytes. Matrix deposition and turnover around cells in control explants was spatially dependent, with the highest rates of proteoglycan deposition and turnover and the lowest rates of collagen deposition (as indicated by [3H]proline autoradiography) occurring in the pericellular matrix. Injurious compression was associated with (a) an abrupt decrease in the tensile load-carrying capacity of the collagen matrix, apparently associated with mechanical failure of the tissue, (b) a considerable but subtotal decrease in cell viability, marked by the emergence of an apparently inactive cell population interspersed within catabolically active but abnormally large cells, and (c) sustained, elevated rates of proteoglycan turnover, particularly in the cell-associated matrices of apparently viable cells, which involved the increased release of aggregating species in addition to a spectrum of degradation fragments that were also in controls. These results may represent an in vitro model for the responses of chondrocytes and the cartilage extracellular matrix to mechanical injury.     

2-Deoxy-D-glucose preferentially kills multidrug-resistant human KB carcinoma cell lines by apoptosis

The aim of this study was to determine the mechanism of cell death associated with the preferential killing of multidrug-resistant (MDR) cells by the glycolytic inhibitor 2-deoxy-D-glucose (2DG) in a range of MDR human KB carcinoma cell lines selected in different drugs. The D10 values for KB-V1, KB-C1 and KB-A1 (selected in vinblastine, colchicine and doxorubicin respectively) were 1.74, 1.04 and 0.31 mM, respectively, compared with 4.60 mM for the parental cell line (KB-3-1). The mechanism of cell death was identified as apoptosis, based on nuclear morphology, annexin V binding and poly(ADP-ribose) polymerase (PARP) cleavage. 2DG induced apoptosis in the three MDR cell lines in a dose- and time-dependent manner and did not induce necrosis. PARP cleavage was detected in KB-C1 cells within 2 h of exposure to 50 mM 2DG and slightly later in KB-A1 and KB-V1 cells. The relative levels of 2DG sensitivity did not correlate with the levels of multidrug resistance or with the reduced levels of the glucose transporter GLUT-1 in these cells. We speculate that a 2DG-stimulated apoptotic pathway in MDR KB cells differs from that in normal KB cells.     

The coalescence of H2S and CO2 bubbles in water

Coalescence times are reported for bubbles of H₂S and CO₂ formed on adjacent nozzles in water. With CO₂, times were small (1–3 ms) at pressures below 2 MPa, and rose to 20 ms at 3.4 MPa. With H₂S, the logarithm of the coalescence time was proportional to pressure, and coalescence times reached 100 ms at 1.7 MPa. Film stretching rates were calculated assuming that the film surfaces were slightly immobile. By using a form of Vrij's theory for the inherent breaking time of films of constant thickness, the effective number of immobile layers could be calculated. Since these were in the range 10^?2 to 10^?3, only a small amount of “stiffness” in the film surface is enough to cause large changes in coalescence time.     

PLUGE method of adjusting picture monitors in television studios

The PLUGE method is described, and its advantages are given. Five sets of reference conditions for setting up picture monitors are presented. Further, it is shown that the required PLUGE black level is dependent upon the value of base luminance.     

THE ESTIMATION OF RANDOM COEFFICIENT AUTOGRESSIVE MODELS. II

Abstract. In Nicholls and Quinn (1980) a procedure was proposed for the determination of strongly consistent estimates of random coefficient autoregressive models. These estimates are used here as starting values in a Newton-Raphson algorithm which is employed to obtain the maximum likelihood estimates of a class of random coefficient autoregressions. The maximum likelihood estimates are shown to be strongly consistent and to satisfy a central limit theorem. The problem of testing for the randomness of the coefficients is also briefly discussed. The results of a number of simulations are reported which illustrate the theoretical results obtained.