Three-dimensional reconstruction from a single-exposure,random conical tilt series applied to the 50S ribosomal subunit of Escherichia coli

We present a new reconstruction method that takes advantage of the fact that many biological macromolecular assemblies show a preferred orientation with respect to the plane of the specimen grid in the electron microscopic preparation. From one micrograph taken of such a specimen tilted by a large angle, a conical tilt series with random azimuthal angles can be extracted and used for a three-dimensional reconstruction. Our technique allows the determination of the molecular structure under low-dose conditions, which are not achievable with reconstruction methods that use conventional tilt series. The reconstruction method combines a number of existing image processing techniques with a newly developed weighted back-projection algorithm designed for three-dimensional reconstruction from projections taken with arbitrary projecting directions. The method is described as it was applied to the three-dimensional reconstruction of the structure of the 50S ribosomal subunit of Escherichia coli (E. coli).     

Comprehensive investigation of numerical methods in simulating a steady-state vapor compression system

Computer simulation has become a required tool in the design phase of vapor compression systems; however with relatively few exceptions most simulations focus on the basic four component systems. With an increasing focus being placed on energy efficiency, the simulation of multi-component vapor compression systems (having multiple evaporators, condenser or compressors) will become essential to assist in the design of these more complicated systems. The implementation of a component-based framework will facilitate the simulation of multi-component systems. This paper describes three algorithms used to simulate a component-based vapor compression system. A test matrix of 6174 sample runs covering a wide range of operating conditions was constructed to determine the robustness and speed of each method when using three different types of nonlinear equation solvers. Each method was tested by simulating a basic four component cycle and a more advanced multiple evaporator system. The results are presented in such a format as to describe the reasons that contribute to any instability of the solvers and the computational efficiency of each method is discussed.     

A Novel Application of Thermoelectric Modules in an HVAC System Under Cold Climate Operation

A vapor compression cycle (VCC) with integrated thermoelectric (TE) modules to boost the heating capacity of the system in an energy-efficient way, especially for cold climate operation, is suggested in this paper. While a baseline heat pump (HP) cycle absorbs heat from a source through an evaporator, the proposed system utilizes TE modules as an intermediate (or third) stage of an otherwise two-stage vapor compression system with a vapor injection compressor. This increases the overall system efficiency and augments the system capacity through the high coefficient of performance (COP) of the TE for small temperature lift conditions. To demonstrate the concept, a prototype refrigerant-to-solid (TE) heat exchanger, consisting of TE modules and microchannel flat tubes, was designed and fabricated so that the whole system could realize an additional 1?kW of heating capacity compared with the baseline system. The TE heat exchanger was integrated into a residential HP unit that uses R-410A as a refrigerant, and the system was tested in a laboratory under the severe condition of ?17.8°C, in order to investigate the capacity improvement and the overall COP. Finally, an application of this technique in an automotive heating, ventilating, and air-conditioning system with HFC134a working fluid has been studied for the purpose of providing supplemental heating for electric vehicles and hybrid electric vehicles by establishing a detailed simulation model of a HP system with the TE heat exchanger. Both the laboratory test and the calculation study show that a VCC with integrated TE modules has both reasonable efficiency and increased heating capacity.     

Field performance measurements of a heat pump desiccant unit in heating and humidification mode

In this study, a novel self-regenerating electric vapor compression heat pump desiccant (HPD) unit operated in the heating and humidification mode during the winter season is introduced. The HPD unit was installed in an office suite for the field test. The performance of the HPD unit and the provided indoor conditions were measured over a wide range of operating conditions. The target indoor humidity ratio was set to 4.4 g/kg, which is the minimum required indoor humidity ratio for a comfortable indoor environment indicated in the ASHRAE winter thermal comfort zone. The seasonal comparison revealed that even though 77.7% of all outdoor humidity ratio data was lower than 4.4 g/kg, 78.2% and 85.8% of all the indoor humidity ratio data of each room were found to be higher than 4.4 g/kg. In addition, due to the significant sensible capacity of the HPD unit, the indoor temperatures could be maintained within 20-25 °C. These results prove that the HPD unit not only properly humidifies the indoors without using any additional water source, like the conventional humidifier, but also helps to keep the indoor temperature at the desired temperature levels.     

Simulation comparison of VAV and VRF air conditioning systems in an existing building for the cooling season

Performance of two widely used air conditioning (AC) systems, variable air volume (VAV) and variable refrigerant flow (VRF), in an existing office building environment under the same indoor and outdoor conditions for an entire cooling season is simulated by using two validated respective models and compared. It was observed that the indoor temperatures could not be maintained properly at the set temperature by the VAV no-reheat boxes. However, it could be maintained by the VAV boxes with reheat with a significant energy consumption penalty. It was found that the secondary components (indoor and ventilation units) of the VRF AC system promised 38.0-83.4% energy-saving potential depending on the system configuration, indoor and outdoor conditions, when compared to the secondary components (heaters and the supply fan) of the VAV AC system. Overall, it was found that the VRF AC system promised 27.1-57.9% energy-saving potentials depending on the system configuration, indoor and outdoor conditions, when compared to the VAV AC system.     

Two-stage heat pump system with vapor-injected scroll compressor using R410A as a refrigerant

Refrigerant vapor-injection technique has been well justified to improve the performance of systems in refrigeration applications. However, it has not received much attention for air conditioning applications, particularly for air conditioning in hot climates and for heat pumping in cold climates. In this study, the performance of an 11 kW R410A heat pump system with a two-stage vapor-injected scroll compressor was experimentally investigated. The vapor-injected scroll compressor was tested with the cycle options of both flash tank and internal heat exchanger configurations. A cooling capacity gain of around 14% with 4% COP improvement at the ambient temperature of 46.1 °C and about 30% heating capacity improvement with 20% COP gain at the ambient temperature of −17.8 °C were found for the vapor-injected R410A heat pump system as compared to the conventional system which has the same compressor displacement volume.     

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Effects of epinephrine on glucose metabolism in patients with alcoholic cirrhosis

The cirrhotic liver has been shown to be resistant to the actions of various glucoregulatory hormones. The objective of this study was to investigate the effects of epinephrine on hepatic glucose metabolism in cirrhotic patients. Thirteen cirrhotic and eight healthy subjects were studied. Hepatic glucose production and turnover of alanine and glycerol were measured using stable isotope technique before and during 70 and 150 minutes of epinephrine infusion (0.1 microgram/kg/min). beta-Adrenoreceptor binding sites and affinity in mononuclear leukocyte membranes also were determined. Hepatic glucose production and alanine turnover in normals significantly increased during epinephrine infusion, but did not change in cirrhotics. Glycerol turnover increased after 70 minutes of epinephrine infusion in both groups. Epinephrine induced a significant rise of high-affinity beta-adrenoreceptor binding sites in normals, yielding a significant correlation between hepatic glucose production and receptor density (r = .94, P < .0001). In cirrhotic patients, similar changes in the number of high-affinity beta-adrenoreceptors were observed, but no correlation with hepatic glucose production was detected. The cirrhotic liver did not respond normally to the stimulatory effect of epinephrine on hepatic glucose production. Because this blunted response was not related to changes of beta-adrenoreceptors, our findings suggest that epinephrine resistance in cirrhosis was caused by a postreceptor defect.     

POD‐based model reduction with empirical interpolation applied to nonlinear elasticity

The constantly rising demands on finite element simulations yield numerical models with increasing number of degrees‐of‐freedom. Due to nonlinearity, be it in the material model or of geometrical nature, the computational effort increases even further. For these reasons, it is today still not possible to run such complex simulations in real time parallel to, for example, an experiment or an application. Model reduction techniques such as the proper orthogonal decomposition method have been developed to reduce the computational effort while maintaining high accuracy. Nonetheless, this approach shows a limited reduction in computational time for nonlinear problems. Therefore, the aim of this paper is to overcome this limitation by using an additional empirical interpolation. The concept of the so‐called discrete empirical interpolation method is translated to problems of solid mechanics with soft nonlinear elasticity and large deformations. The key point of the presented method is a further reduction of the nonlinear term by an empirical interpolation based on a small number of interpolation indices. The method is implemented into the finite element method in two different ways, and it is extended by using different solution strategies including a numerical as well as a quasi‐Newton tangent. The new method is successfully applied to two numerical examples concerning hyperelastic as well as viscoelastic material behavior. Using the extended discrete empirical interpolation method combined with a quasi‐Newton tangent enables reductions in computational time of factor 10 with respect to the proper orthogonal decomposition method without empirical interpolation. Negligibly, orders of error can be reached. Copyright © 2015 John Wiley & Sons, Ltd.