The removal of Escherichia coli-bacteriophage T7 by magnetic filtration

Magnetic filtration effectively removed bacteriophage T₇ from water. The removal was undertaken by adsorbing the virus onto magnetite in the presence of calcium chloride and by passing the mixture through a filter placed in a magnetic field.     

Determination of methionine and selenomethionine in yeast by species-specific isotope dilution GC/MS

A method for the simultaneous determination of methionine (Met) and selenomethionine (SeMet) in yeast using species-specific isotope dilution (ID) gas chromatography/mass spectrometry (GC/MS) is described. Samples were digested by refluxing for 16 h with 4 M methanesulfonic acid. Analytes were derivatized with methyl chloroformate and extracted into chloroform for GC/MS analysis. In addition to use of commercially available 13C-enriched Met and SeMet spikes for species specific ID analysis, a 74Se-enriched SeMet spike was also available for comparison of results. In selective ion monitoring mode, the intensities of ions at m/z 221, 222, 269, 270, and 263 were used to calculate the 221/222, 269/270, and 269/263 ion ratios for quantification of Met and SeMet. Concentrations of 5959 +/- 33 and 3404 +/- 12 microg g(-1) (one standard deviation, n = 6) with relative standard deviations of 0.55 and 0.36% for Met and SeMet, respectively, were obtained using 13C-enriched spikes. A concentration of 3417 +/- 8 microg g(-1) (one standard deviation, n = 6) was obtained using the 74Se-enriched SeMet spike. The concentration of SeMet measured in the yeast is equivalent to 66.43 +/- 0.24% of total Se and 30.31 +/- 0.11% of total Met is in the form of SeMet. Method detection limits (three times the standard deviation) of 3.4 and 1.0 microg g(-1) were estimated for Met and SeMet, respectively, based on a 0.25-g subsample of yeast with 1 mL of extract used for derivatization. A similar concentration of 5930 +/- 29 microg g(-1) (one standard deviation, n = 4) for Met and a lower concentration of 2787 +/- 49 microg g(-1) (one standard deviation, n = 4) for SeMet were obtained for this yeast sample using species-specific ID analysis based on GC/MS with 13C-enriched Met and SeMet spikes when a 2-h open microwave digestion approach using 8 M methanesulfonic acid was used.     

Evaluation of outpatient psychotherapy: Some suggested guidelines.

Suggests guidelines for developing evaluative procedures to fit the needs and constraints of specific treatment settings. The orientation is practical, recognizing that clients and professionals alike must carefully attend to cost–benefit factors. It is felt that psychotherapists can move beyond their current impressionistic, global evaluations and develop procedures with greater value for corrective feedback and communication to both lay and professional audiences. (8 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Solar radiation incident upon slopes of different orientations

By means of a pyranometer oriented at various tilt angles and azimuth angles with respect to the Sun, measurements of the direct and diffuse solar flux incident on slopes of various orientations have been made for clear sky conditions. The resulting data have been used to evaluate the validity of the solar flux model of Robinson[1] and to introduce certain modifications of that model to achieve better agreement of computations with measurements. It is concluded that the modified model yields significantly better results than the original for slopes oriented in the azimuth of the Sun, but results are comparable for other azimuthal orientations of the slope. The accuracy attainable by this modeling approach is considered sufficient for many practical applications.     

A generalized model for Kalman filtering of motion information in video compression

In this paper, we tackle the problem of motion estimation in video compression. Since Full Search Algorithms (FSA) present the disadvantage of adding a high computational burden to the encoder, fast search techniques have been used in conjunction with predictive filtering, in such a way to guarantee an acceptable quality with an affordable complexity. The aim of this work is to propose a novel framework for Kalman filtering of motion information in compressed video sequences. The merits of our new framework are twofold: First, using an appropriate formulation of the system equations, several shortcomings inherent with former models in the literature are greatly counteracted. Secondly, it is constructed using a generalized structure in such a way to enclose a large variety of prediction models. Therefore, it can adapt to different types of motion activities in video sequences, without the need for a different formulation in each prediction model, as was the case in previous studies. Furthermore, we propose an adaptive motion compensation technique that permits an additional improvement to the decoded video quality. Our framework permits a considerable gain in the average performance compared to previous models and even to the FSA technique.     

Dynamic Mechanical Analysis of Amorphous-Phase Organization in Acrylic Polymers

Factors affecting polymer network organization were studied in highly crosslinked acrylics of the type used in dental adhesive resins. The variables tested were comonomer content and processing conditions. BisEMA (2,2,-bis[4-(2-methacryloyloxyethoxy)-phenyl]-propane) and BisEMA + TEGDMA (triethyleneglycol dimethacrylate) were cured with and without 25% comonomer. Comonomers had characteristics that are expected to influence intrachain organization in amorphous phases: TEGDMA, crosslinking; methyl methacrylate (MMA), monomer conversion; isobornyl methacrylate (IBM), low cure shrinkage; tetrahydrofurfuryl methacrylate (THFM), antiplasticization. Dynamic mechanical analysis temperature scans were run at 0.1 Hz 2h or 24h after ambient cure, or 24h postcure after heating at 75° or 125°C. After 24h, tan δ maxima occurred in ranges centering on approximately -30°, 75° and 150°C (T_g). Heating at 125°C nearly eliminated all peaks except T_g, reduced tan δ peaks and increased T_g by 0–14°. T_g increased in the order: TEGDMA>125°C>IBM>MMA>75°C>2h>24h>THFM. The ability to crosslink, and postcure heating at 125°C, were the more important factors found to increase intrachain organization in amine-promoted, unfilled BisEMA resins of the type used in dental sealants, luting cements and bulk-filling resin composite materials.