Preparation and in vitro characterization of gentamycin-impregnated biodegradable beads suitable for treatment of osteomyelitis

A new method for preparing poly(L-lactide) (PLA) biodegradable beads impregnated with an ionic aminoglycoside, gentamycin, is described. The process employs hydrophobic ion pairing to solubilize gentamycin in a solvent compatible with PLA, followed by precipitation with a compressed antisolvent (supercritical carbon dioxide). The resulting precipitate is a homogeneous dispersion of the ion-paired drug in PLA microspheres. The microspheres are approximately 1 microm in diameter and can be compressed into beads (3-6 mm in diameter) strung on surgical sutures for implantation. The bead strings exhibit no significant change in release kinetics upon sterilization with a hydrogen peroxide plasma (Ster-Rad). The kinetics of gentamycin release from the PLA beads are consistent with a matrix-controlled diffusion mechanism. While nonbiodegradable poly(methyl methacrylate) (PMMA) beads initially release gentamycin in a similar manner, the drug release from PMMA ceases after 8 or 9 weeks, while the PLA beads continue to release drug for over 4 months. Moreover, only 10% of the gentamycin is released from the PMMA beads, while PLA beads release more than 60% of their load, if serum is present in the release medium. The PLA system displays improved release kinetics relative to PMMA, is biodegradable, is unaltered by gas sterilization, can be used for a range of antibiotics, and can be manipulated without disintegration. These are all desirable properties for an implantable drug delivery system for the prevention or treatment of osteomyelitis.     

Centrifuge Modeling of the Cyclic Lateral Response of a Rigid Pile in Soft Clay

A series of centrifuge model tests of the lateral response of a fixed-head single pile in soft clay is reported. Both monotonic and cyclic episodes of loading are described, with varying amplitude and with intervening periods of reconsolidation. The soil conditions are characterized by cyclic T-bar penetrometer tests. The ultimate capacity under monotonic load for virgin and for postcyclic conditions was found to be comparable with calculations based on existing design methods, including theoretical plasticity solutions and empirical methods. The lateral stiffness was observed to degrade with cycles, with the rate of degradation being greater for larger cycles. The degradation pattern has been tentatively linked to the cyclic T-bar response, by considering the ‘damage’ associated with the cumulative displacement and remolding, in each case. This approach provides a consistent interpretation of the tests. Although episodes of pile movement and soil remolding led to a reduction in lateral resistance, intervening periods of reconsolidation led to a similar magnitude of recovery and a reduction in the level of softening in subsequent cyclic episodes. During an initial episode of cyclic lateral movement, the stiffness degraded by a factor of 2.3, which is comparable with the strength sensitivity derived from a cyclic T-bar test. In contrast, after five episodes of reconsolidation, the stiffness had recovered back to within 25% of the stiffness observed in the first cycle of the first episode, and it showed negligible degradation during subsequent cycling. This observation implies that, over a long period of cyclic loading, the lateral stiffness of a pile may tend towards a value that is independent of cycle number, and that represents a balance between the damaging effects of remolding and pore pressure generation and the healing effects of time and reconsolidation.     

Quantification of Losses of Labor Efficiencies: Innovations in and Improvements to the Measured Mile

This paper describes a rigorous procedure for quantifying damages arising from loss of labor inefficiency. Throughout, deficiencies with the measured mile concept are cited. Perhaps one of the least recognized deficiencies is that the measured mile is a concept, not a procedure. Thus, every analyst is left to conduct the study as he/she pleases. This paper defines a step-by-step process for doing a damage study, and cites rules to follow when performing the study. Following the procedure and rules will lead to more rigorous and defensible analyses. A baseline analysis is described and presented as a way of adhering to the measured mile concept. The principle difference is in how best performance is defined. In a baseline study, the focus is on periods of high output (production), not good productivity. The baseline periods need not be continuous. Also, the analysis is performed on combined data (one account), not many accounts. The calculations used to perform a baseline study are illustrated using a simple case study project. Reasons for recovering damages because of losses of labor efficiency are also cited.     

Bearing Response of Skirted Foundation on Nonhomogeneous Soil

Circular skirted offshore foundations on nonhomogeneous soil have been studied numerically, analytically, and physically, with the offshore sediment simulated as a cohesive soil with strength increasing linearly with depth. In the numerical analysis, the h-adaptive FEM is adopted to provide an optimal mesh, in which a strain-superconvergent patch recovery error estimator and mesh refinement with subdivision concept are used. This paper presents two main studies of circular skirted foundations on nonhomogeneous soil, consisting of a bearing-capacity study and a large penetration study. The bearing capacity of the foundations is studied with the degree of nonhomogeneity (kD∕suo) of soil up to 30, different skirt roughness and skirt depth up to five times the foundation diameter (i.e., Df∕D = 5), using an h-adaptive FEM and extended upper-bound method. The bearing-capacity values and soil flow mechanisms are discussed. In the foundation large penetration study, circular skirted foundations penetrating into normally consolidated and overconsolidated soils are tested physically in the centrifuge and analyzed numerically using the h-adaptive remeshing and interpolation technique with small strain method for soil large deformation analysis. The load-displacement responses from centrifuge test data and finite-element results are compared.     

Loss in Anchor Embedment during Plate Anchor Keying in Clay

Vertically installed plate anchors have been investigated in this paper by numerical analysis and centrifuge modeling. In the numerical analysis, the large deformation finite-element method (remeshing and interpolation technique with small strain) was used to simulate strip plate anchor rotation. In the centrifuge model tests, transparent soils were used to observe square anchor rotation. The loss in anchor embedment during anchor keying was assessed for anchors in uniform and normally consolidated soils with anchor pullout angle varying from 30° to 90° to the horizontal. It was found that the loss in anchor embedment during anchor keying may be expressed in terms of a nondimensional anchor geometry factor, which is a function of the eccentricity of the padeye, angle of loading, and the net moment applied to the anchor at the stage where the applied load balances the anchor weight. However, once the anchor geometry factor reaches a certain value, the loss in anchor embedment stabilizes at 0.25–0.5 times the anchor width. The loss in anchor embedment decreases linearly with decreasing pullout angle. Simple formulae and design procedures have been proposed to estimate the loss in anchor embedment during keying.     

Setup Following Installation of Dynamic Anchors in Normally Consolidated Clay

This paper describes a series of centrifuge model tests designed to assess the increase in capacity of dynamic anchors due to setup in normally consolidated clay. The tests involved measurement of the vertical capacity of 1:200 reduced scale model anchors following various periods of postinstallation consolidation. The short-term capacity was shown to be dependent on the anchor impact velocity. Cavity expansion solutions for consolidation around a solid driven pile were found to provide agreement with the experimental results. A simplified capacity calculation technique predicted higher friction ratio values than is typically observed for driven piles; however, these calculations were complicated by the unusual dynamic anchor load–displacement response and uncertainty regarding the true sample shear strength. Dynamic anchor consolidation proceeds at a slower rate than for suction caissons and open-ended piles of similar equivalent diameter. However, the results indicate that depending on the site conditions, dynamically installed anchors remain a viable alternative to conventional deep-water mooring techniques.     

Ultrasensitive detection and characterization of posttranslational modifications using surface-enhanced Raman spectroscopy

Posttranslational modification (PTM) of proteins is likely to be the most common mechanism of altering the expression of genetic information. It is essential to characterize PTMs to establish a complete understanding of the activities of proteins. Here, we present a sensitive detection method using surface-enhanced Raman spectroscopy (SERS) that can detect PTMs from as little as zeptomoles of peptide. We demonstrate, using model peptides, the ability of SERS to detect a variety of protein modifications, such as acetylation, trimethylation, phosphorylation, and ubiquitination. In addition, we show the capability to obtain positional information for modifications such as trimethylation and phosphorylation using SERS and wavelet decomposition data analysis techniques. We further show that it is possible to apply SERS to detect PTMs from biological samples such as histones. We envision that this detection method might be a valuable technique that is complementary to mass spectrometry in obtaining orthogonal chemical and modification-specific information from biological samples at sensitive levels.     

Microstructural Stability and Hot Deformation of γ–γ′–δ Ni-Base Superalloys

Nickel-base superalloys exhibit excellent high-temperature mechanical and physical properties and remain the first choice for structural components in advanced gas turbine engines for the aerospace propulsion and power generation applications. In response to the increasing demand for more efficient solutions and tighter requirements linked to gas turbine technologies, the properties of nickel-base superalloys can be improved by modification of their thermo-mechanical and/or compositional attributes. Recent investigations have revealed the potential use of ternary eutectic γ–γ′–δ Ni-base superalloys in advanced gas turbines due to high temperature mechanical properties that are comparable to state-of-the-art polycrystalline Ni-base superalloys. With properties largely dependent on microstructural strengthening mechanisms, both the composition and thermo-mechanical processing parameters of this novel class of alloys need to be optimized concurrently. The hot deformation characteristics of four γ–γ′–δ Ni-base superalloys with varying levels of Nb were evaluated at temperatures and strain rates between 1353 K and 1433 K (1080 °C and 1160 °C) and 0.01 to 0.001/s, respectively. Evidence of dislocation-based plasticity was observed following deformation at low temperatures and high strain rates, while high temperatures and low strain rates promoted superplasticity in these alloys. The extent of the microstructural changes and the magnitude of the cavitation damage which occurred during deformation was found to vary as a function of the alloy composition.     

Generation(s) of phonological codes in reading: A reply to Guttentag.

Addresses R. E. Guttentag's (see record 1982-11297-001) 2 questions about the review by L. X. McCusker et al (see record 1981-11846-001) of the phonological recoding literature. Following Guttentag's suggestion, the possibility of phonological mediation without the use of grapheme–phoneme correspondence rules is considered. It is recognized as a logically possible supplement to phonological recoding, but no empirical support was found. Guttentag's discussion of how dependence on phonological recoding might change with increased age and reading skill is also addressed. (12 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)