Concerted evolution at the population level: pupfish HindIII satellite DNA sequences

The canonical monomers (approximately 170 bp) of an abundant (1.9 x 10(6) copies per diploid genome) satellite DNA sequence family in the genome of Cyprinodon variegatus, a "pupfish" that ranges along the Atlantic coast from Cape Cod to central Mexico, are divergent in base sequence in 10 of 12 samples collected from natural populations. The divergence involves substitutions, deletions, and insertions, is marked in scope (mean pairwise sequence similarity = 61.6%; range = 35-95.9%), is largely confined to the 3' half of the monomer, and is not correlated with the distance among collecting sites. Repetitive cloning and direct genomic sequencing experiments failed to detect intrapopulation and intraindividual variation, suggesting high levels of sequence homogeneity within populations. The satellite sequence has therefore undergone "concerted evolution," at the level of the local population. Concerted evolution has previously almost always been discussed in terms of the divergence of species or higher taxa; its intraspecific occurrence apparently has not been reported previously. The generality of the observation is difficult to evaluate, for although satellite DNAs from a large number of organisms have been studied in detail, there appear to be little or no other data on their sequence variation in natural populations. The relationship (if any) between concerted, population level, satellite DNA divergence and the extent of gene flow/genetic isolation among conspecific natural populations remains to be established.     

Recent advances in amperometric glucose biosensors for in vivo monitoring

The substance-use, positive health practices, diet and eating habits, driving behaviours and preventive health care practices of 113 nurses living in the southeast of England were assessed by means of a postal questionnaire. Females were generally more compliant than males with most of the behaviours assessed. Females reported taking greater risks with driving behaviours by an occasional tendency to "drink and drive". Many males reported both a lack of knowledge of how to self-examine their testicles for signs of cancer and little compliance with this behaviour. This sample reported more smoking and alcohol use than previously reported samples of American nurses and U.K. norms. The nurses in this sample were more likely to avoid foods high in cholesterol, add fibre to their diet and be more vigilant in their driving behaviour than American nurses and British non-nurses.     

Highly Efficient Quasi 2D Blue Perovskite Electroluminescence Leveraging a Dual Ligand Composition

Perovskite light-emitting diodes (PeLEDs) are advancing because of their superior external quantum efficiencies (EQEs) and color purity. Still, additional work is needed for blue PeLEDs to achieve the same benchmarks as the other visible colors. This study demonstrates an extremely efficient blue PeLED with a 488 nm peak emission, a maximum luminance of 8600 cd m⁻², and a maximum EQE of 12.2% by incorporating the double-sided ethane-1,2-diammonium bromide (EDBr₂) ligand salt along with the long-chain ligand methylphenylammonium chloride (MeCl). The EDBr₂ successfully improves the interaction between 2D perovskite layers by reducing the weak van der Waals interaction and creating a Dion–Jacobson (DJ) structure. Whereas the pristine sample (without EDBr₂) is inhibited by small stacking number (n) 2D phases with nonradiative recombination regions that diminish the PeLED performance, adding EDBr₂ successfully enables better energy transfer from small n phases to larger n phases. As evidenced by photoluminescence (PL), scanning electron microscopy (SEM), and atomic force microscopy (AFM) characterization, EDBr₂ improves the morphology by reduction of pinholes and passivation of defects, subsequently improving the efficiencies and operational lifetimes of quasi-2D blue PeLEDs.     

Energy sector planning using multiple-index pinch analysis

Pinch analysis was initially developed as a methodology for optimizing energy efficiency in process plants. Applications of pinch analysis applications are based on common principles of using stream quantity and quality to determine optimal system targets. This initial targeting step identifies the pinch point, which then allows complex problems to be decomposed for the subsequent design of an optimal network using insights drawn from the targeting stage. One important class of pinch analysis problems is energy planning with footprint constraints, which began with the development of carbon emissions pinch analysis; in such problems, energy sources and demands are characterized by carbon footprint as the quality index. This methodology has been extended by using alternative quality indexes that measure different sustainability dimensions, such as water footprint, land footprint, emergy transformity, inoperability risk, energy return on investment and human fatalities. Pinch analysis variants still have the limitation of being able to use one quality index at a time, while previous attempts to develop pinch analysis methods using multiple indices have only been partially successful for special cases. In this work, a multiple-index pinch analysis method is developed by using an aggregate quality index, based on a weighted linear function of different quality indexes normally used in energy planning. The weights used to compute the aggregate index are determined via the analytic hierarchy process. A case study for Indian power sector is solved to illustrate how this approach allows multiple sustainability dimensions to be accounted for in energy planning.     

Discriminating constant from variable angular velocities in structure from motion

We investigated accuracy in discriminating between constant and variable angular velocities for orthographic projections of three-dimensional rotating objects. The reported judgments of "constant" or "variable" angular velocity were only slightly influenced by the projected angular velocities, but they were greatly affected by the variations of the deformation, a first-order component of the optic flow. When viewing either a rotating ellipsoidal volume or a planar surface that accelerated and decelerated over the course of rotation, observers' tendencies to report a variable angular velocity were increased when the temporal phase of the acceleration pattern increased the range of variation of the median deformation; the tendencies were decreased when the same acceleration pattern was used to decrease the range of variation of the median deformation. These results provide evidence contrary to the hypothesis that the visual system performs a mathematically correct analysis of the optic flow.     

Fiber-optic probes with improved excitation and collection efficiency for deep-UV Raman and resonance Raman spectroscopy

The ability of ultraviolet resonance Raman spectroscopy (UVRRS) to determine structural, environmental, and analytical information concerning low-concentration aqueous biomolecules makes it a powerful bioanalytical and biophysical technique. Unfortunately, its utility has been limited by experimental requirements that preclude in situ or in vivo studies in most cases. We have developed the first high-performance fiber-optic probes suitable for long-term use in pulsed UVRRS applications in the deep- UV (DUV, 205-250 nm). The probes incorporate recently developed improved ultraviolet (IUV) fibers that do not exhibit the rapid solarization and throughput decay that previously hampered the use of optical fibers for delivering pulsed, DUV light. A novel 90 degrees mirrored collection geometry is used to overcome the inner-filtering effects that plague flush-probe geometries. The IUV fibers are characterized with respect to their efficacy at transmitting pulsed, DUV laser light, and prototype probes are used to obtain pulsed UVRRS data of aromatic amino acids, proteins, and hormones at low concentrations with 205-240-nm pulsed excitation. Efficient probe geometries and fabrication methods are presented. The performance of the probes in examining resonance-enhanced Raman signals from absorbing chromophores is investigated, and the optimal excitation wavelength is shown to be significantly red-shifted from the maximum of the resonance Raman enhancement profile. Generally applicable procedures for determining optimal experimental conditions are also introduced.     

1996 editorial: 50th Anniversary Issue of the American Psychologist.

Presents an editorial on the 50th Anniversary Issue of The American Psychologist by the CEO and Executive Vice President of the American Psychological Association. An overview of changes to the journal and the developments in electronic communications pare presented, and the implications for international exchange in scholarly publishing are discussed. (0 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Proteomics in chronic kidney disease: The issues clinical nephrologists need an answer for

A growing number of patients are recognised to have chronic kidney disease (CKD). However, only a minority will progress to end-stage renal disease requiring dialysis or transplantation. Currently available diagnostic and staging tools frequently fail to identify those at higher risk of progression or death. Furthermore within specific disease entities there are shortcomings in the prediction of the need for therapeutic interventions or the response to different forms of therapy. Kidney and urine proteomic biomarkers are considered as promising diagnostic tools to predict CKD progression early in diabetic nephropathy, facilitating timely and selective intervention that may reduce the related health-care expenditures. However, independent groups have not validated these findings and the technique is not currently available for routine clinical care. Furthermore, there are gaps in our understanding of predictors of progression or need for therapy in non-diabetic CKD. Presumably, a combination of tissue and urine biomarkers will be more informative than individual markers. This review identifies clinical questions in need of an answer, summarises current information on proteomic biomarkers and CKD, and describes the European Kidney and Urine Proteomics initiative that has been launched to carry out a clinical study aimed at identifying urinary proteomic biomarkers distinguishing between fast and slow progressors among patients with biopsy-proven primary glomerulopathies.     

Examining knowledge contribution from the perspective of an online identity in blogging communities

Knowledge contribution is one of the essential factors behind the success of blogging communities (BCs). This research studies knowledge contribution behavior in a BC from the perspective of knowledge contributors and their characteristics using the lens of social identity theory. Social identity theory asserts that individuals are fundamentally motivated to present or communicate their identities in everyday social life through behavior. A similar line of reasoning can be used to argue that members of a BC would also be motivated to communicate their online identities through their behavior, that is, through knowledge contribution in the BC. Specifically, this study conceptualized the online identity and examined the effects of its personal (online kindness, online social skills, and online creativity) and social aspects (BC involvement) on knowledge contribution. The data was collected using an online survey from the members of Cyworld, a popular BC in South Korea and a few other countries (members from South Korea were included in this study). The results indicate that both the personal and social aspects of online identity and their interactions significantly influenced knowledge contribution. Based on the findings, this study offers suggestions to organizers of BCs to enhance the knowledge contribution from their members.     

Shape optimization for tumor location

In non-invasive thermal diagnostics, accurate correlations between the thermal image at skin surface and interior human physiology are desired. In this work, an estimation methodology to determine unknown geometrical parameters of an embedded tumor is proposed. We define a functional that represents the mismatch between a measured experimental temperature profile, which may be obtained by infrared thermography on the skin surface, and the solution of an appropriate boundary problem. This functional is related to the geometrical parameters through the solution of the boundary problem, in such a way that finding the minimum of this functional form also means finding the unknown geometrical parameters of the embedded tumor. Sensitivity analysis techniques coupled with the adjoint method were considered to compute the shape derivative of the functional. Then, a nonmonotone spectral projected gradient method was implemented to solve the optimization problem of finding the optimal geometric parameters.