Assessment of iron bioavailability from twenty elite late‐maturing tropical maize varieties using an in vitro digestion/Caco‐2 cell model

An in vitro digestion/Caco‐2 cell model was used to assess iron bioavailability of twenty elite late‐maturing tropical maize varieties grown in three diverse agroecologies in West and Central Africa (WCA). Kernel‐iron concentration of the varieties, averaged across locations, varied from 19.2 to 24.4 mg kg^?1, while mean kernel‐zinc concentration ranged between 19.4 and 24.6 mg kg^?1. Significant differences in iron bioavailability were observed among varieties, but the environment had no significant effect. Mean bioavailable iron ranged between 14% below and 43% above the reference control variety, TZB‐SR. Variety DMR‐LSR‐Y with the highest concentrations of kernel‐iron and ‐zinc of 24–25 mg kg^?1 across the three locations had a similar quantity of bioavailable iron as the reference control, TZB‐SR. In the long run this variety could be potentially effective in reducing iron deficiency because of its high kernel‐iron. The most promising varieties were Mid‐altitude STR synthetic and ACR91SUWAN‐1‐SRC1. They had kernel‐iron and ‐zinc levels of 22–24 mg kg^?1 and bioavailable iron 24–36% higher than the reference control, TZB‐SR. Additional research is necessary to determine if the increases in kernel‐iron concentration and bioavailable iron observed in this study can significantly improve the iron status of individuals in WCA at risk for iron deficiency. Copyright © 2004 Society of Chemical Industry     

Glycation of the Major Milk Allergen β‐Lactoglobulin Changes Its Allergenicity by Alterations in Cellular Uptake and Degradation

1 Scope

During food processing, the Maillard reaction (МR) may occur, resulting in the formation of glycated proteins. Glycated proteins are of particular importance in food allergies because glycation may influence interactions with the immune system. This study compared native and extensively glycated milk allergen β‐lactoglobulin (BLG), in their interactions with cells crucially involved in allergy.

2 Methods and results

BLG was glycated in MR and characterized. Native and glycated BLG were tested in experiments of epithelial transport, uptake and degradation by DCs, T‐cell cytokine responses, and basophil cell degranulation using ELISA and flow cytometry. Glycation of BLG induced partial unfolding and reduced its intestinal epithelial transfer over a Caco‐2 monolayer. Uptake of glycated BLG by bone marrow–derived dendritic cells (BMDC) was increased, although both BLG forms entered BMDC via the same mechanism, receptor‐mediated endocytosis. Once inside the BMDC, glycated BLG was degraded faster, which might have led to observed lower cytokine production in BMDC/CD4⁺ T‐cells coculture. Finally, glycated BLG was less efficient in induction of degranulation of BLG‐specific IgE sensitized basophil cells.

3 Conclusions

This study suggests that glycation of BLG by MR significantly alters its fate in processes involved in immunogenicity and allergenicity, pointing out the importance of food processing in food allergy.     

Analyzing intention in utterances

This paper describes a model of cooperative behavior and describes how such a model can be applied in a natural language understanding system. We assume that agents attempt to recognize the plans of other agents and, then, use this plan when deciding what response to make. In particular, we show that, given a setting in which purposeful dialogues occur, this model can account for responses that provide more information that explicitly requested and for appropriate responses to both short sentence fragments and indirect speech acts.     

Purity aspects of higher alpha olefins

The various processes which are used commercially to manufacture detergent range alpha olefins are compared in terms of the quality of the products obtained. Thermal cracking ofn-paraffins gives the least pure olefins. Processes based on ethylene oligomerization are superior and, of these, the Shell SHOP (Shell Higher Olefins Process) process gives alpha olefins which are somewhat better in quality than those obtained from processes based on aluminum alkyl chemistry. The practical consequences of the presence of internal and vinylidene olefins, dienes and paraffins in alpha olefins are considered for the manufacture of alpha olefin sulfonates, linear alkyl-benzene, epoxides, alkyl bromides, rnercaptans and copolymers with ethylene (linear low density polyethylene). Low levels of impurities are desirable in most cases to minimize formation of unwanted byproducts, or to reduce the cost of bleeding of inert components, or to ensure that the quality of the final product meets the requirements of the marketplace. Presented at the AOCS Meeting in Toronto, May 1982.     

Synthesis and properties of fatty imidazolines and their N-(2-aminoethyl) derivatives

Cationic high molecular weight surfactants hold promise as water repellents for soil for water harvesting. Specifically, the products of the reaction of 2 moles of fatty acids with 1 mole of diethylenetria-mine were investigated. Optimum conditions for the formation of imidazolines as well as the open-chain N-(2-aminoethyl) derivatives, i.e., the diamides, were determined. Saturated fatty acids, pelargonic through behenic, oleic acid, elaidic acid as well as tallow, tallow fatty acids, and hydrogenated tallow fatty acids, were converted in 6 hr at 150 C to form the diamides. The fatty imidazolines were synthesized by cyclizing the diamides at 150 C for 2 hr under reduced pressure in a 92% yield. The crude imidazolines were purified by crystalliza-tion. The fatty imidazolines were readily hydrolyzed at the C=N bond of the imidazoline ring with water or dilute alcohol. The resulting diamides, RCON(CH₂ CH₂ NH₂)CH₂ CH₂ NHCOR, were also purified by crystallization. Water repellency of these com-pounds was evaluated by contact angle measurements and percola-tion tests. The surface properties of these series of compounds are determined by four parameters. The open-chain diamides are more hydrophobic than the analogous imidazolines, and hydrophobicity increases with increasing molecular weight of derivatives of saturated fatty acids. Oleic acid derivatives are far more hydrophilic than the saturated acid derivatives or the analogous elaidic acid derivatives. The latter are more hydrophilic than stearic derivatives. Presented at the 72nd AOCS annual meeting, New Orleans, 1981.     

Dynamic mechanical analysis of polymers in the liquid state: Use of a perforated shim stock support

This report summarizes some initial results on the use of a perforated brass shim stock support to extend the useful range of dynamic mechanical testing well over 100 degrees above the traditional limiting polymer softening points of T_g or T_m. Weak secondary relaxations were observed above T_g in both polyisobutylene (PIB) and polystyrene (PS) homopolymer, copolymer, and blend systems and above T_m in gutta percha (trans-polyisoprene). The DuPont 981 Dynamic Mechanical Analyzer (DMA)–990 Thermal Analyzer system was used to characterize these weak liquid state processes. The DMA instrument deforms the specimen in flexure, thus minimizing the shear component present in some other techniques. Specimen-coating weights are typically in the range 20–40 mg. The perforations are particularly useful for polymers having low inherent adhesion to metals or systems which are very brittle in the glassy state, e.g., low MW PS's and many methacrylates.     

Distribution of diethylstilboestrol in pigs

The synthetic growth promoter diethylstilboestrol (DES) administered orally to pigs can be detected by radioimmunoassay (RIA) analysis of kidney, liver, faeces, bile and urine from animals fed continuously to slaughter, but not in muscle, fat or plasma. If treated animals are fed on material not containing DES for 72 h prior to slaughter, then the levels of parent compound and metabolite in all products decrease to become not significantly greater than those in control animals. The gross metabolism of DES in pigs and bovines appears to be similar.     

Hydrolytic stability of terephthaloyl chloride and isophthaloyl chloride

The phthaloyl chloride isomers, terephthaloyl chloride (TCl) and isophthaloyl chloride (ICl), are high production volume chemicals used in polymers to impartflame resistance, chemical resistance, and temperature stability and as water scavengers. In these studies, we determined the hydrolytic stability of TCl and ICl and their hydrolysis products in aqueous solutions. Hydrolysis rates for TCl and ICl were initially determined by gas chromatography/flame ionization detection in water buffered at pH 4.0, 7.0, and 9.0 and 0 degrees C for up to 30 min. Subsequent studies determined the products from TCl and ICl hydrolysis. The parent phthaloyl chlorides (TCl and ICl), their intermediate hydrolysis products (designated as the "half-acids"), and their stable hydrolysis products (terephthalic acid (TPA) and isophthalic acid (IPA)) were determined by high-performance liquid chromatography. The half-lives (t(1/2)) of TCl and ICl ranged from an average of 1.2 to 2.2 min and from 2.2 to 4.9 min, respectively, at pH 4-9 and 0 degrees C. The observed first-order rate constants (k(obs)) ranged from an average of 530 to 1100 (x 10(5) s(-1)) for TCl and 240 to 520 (x 10(5) s(-1)) for ICl. Both phthaloyl chlorides formed their respective short-lived intermediates, in which one of the two carboxylic acid chloride functionalities reacts with water to form the carboxylic acid ("half-acid"). Subsequently, the half-acids underwent further hydrolysis so that greater than 90% of the initial phthaloyl chloride hydrolyzed in less than 60 min at 0 degrees C. The hydrolysis products TPA and IPA were hydrolytically stable, undergoing no further transformations after 20 min at pH 7 and 25 degrees C. This work demonstrated that TCl, ICl, and their respective half-acids will not be persistent in aqueous systems for a time sufficient to have a sustained toxicological effect on aquatic organisms (less than 1 h). Performing additional aquatic toxicity studies, biodegradation studies, and potentially mammalian studies on TCl and ICl are unnecessary since the existing information on TPA and IPA with the hydrolysis data presented here is sufficient to address questions on the fate and effects of these two substances in aqueous environments.