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81.
A review of optical, chemical, and biological sensors to detect metabolic activity at the single-cell level is presented in the context of the development of lab-on-a-chip research instrumentation. The sensors reviewed include optical sensors, at both research and commercial levels, that can optically detect intracellular metabolites including adenosine triphosphate, nicotinamide-adenine dinucleotide, reduced flavin adenine dinucleotide, and other metabolites, including oxygen, carbon dioxide, and glucose. Methods to optically detect pH changes which are a general indicator of activity in extracellular space are also briefly reviewed. Performance metrics such as sensitivity, sensor size, drift, time response, and sensing range are included when available. Highly suitable optical sensor technologies for monitoring cellular metabolic activity include luminescent (fluorescent, phosphorescent, and chemiluminescent) and colorimetric optical probes. Different approaches to extracting luminescent and colorimetric information are reviewed, including benchtop techniques, fiber-optic approaches, and the use of probes encapsulated by biologically localized embedding. A brief discussion of alternate optical sensor technologies, such as surface plasmon resonance and infrared absorption spectroscopy, is also presented. 相似文献
82.
Data fitting with a spline using a real-coded genetic algorithm 总被引:2,自引:0,他引:2
Fujiichi Yoshimoto Author Vitae Toshinobu Harada Author Vitae Author Vitae 《Computer aided design》2003,35(8):751-760
To obtain a good approximation for data fitting with a spline, frequently we have to deal with knots as variables. The problem to be solved then becomes a continuous nonlinear and multivariate optimization problem with many local optima. Therefore, it is difficult to obtain the global optimum. In this paper, we propose a method for solving this problem by using a real-coded genetic algorithm. Our method can treat not only data with a smooth underlying function, but also data with an underlying function having discontinuous points and/or cusps. We search for the best model among candidate models by using the Bayes Information Criterion (BIC). With this, we can appropriately determine the number and locations of knots automatically and simultaneously. Five examples of data fitting are given to show the performance of our method. 相似文献
83.
The efficient, economical and often elegant transformation from a design schema to an embodied design solution containing real components is essential for the commercial success of a product. More often the ability of the designer to explore an optimum solution is severely frustrated by the analytically intensive and time-consuming aspects of embodying a concept. This paper presents a computer based system-modelling tool that is to be used by designers during the transformation of a concept to an embodied solution particularly to deal with standard components. This environment provides for the representation of conceptual schemas and subsequent embodiment from computer based component selection modules. These selection modules encompass the full range of electronic representations for standard mechanical components. The strategy for representing a mechanical system, its included components and their associated representations is discussed. The process of constructing a system model, specifying the desired performance characteristics and system resolution are also described. 相似文献
84.
Backlash is one of the most important non-linearities that limit the performance of speed and position control in industrial, robotics, automotive, automation and other applications. The control of systems with backlash has been the subject of study since the 1940s. This survey reveals that surprisingly few control innovations have been presented since the early path breaking papers that introduced the describing function analysis of systems with backlash. Promising developments are however taking place using adaptive and non-linear control strategies. 相似文献
85.
Ph. MullhauptAuthor Vitae B. SrinivasanAuthor VitaeD. BonvinAuthor Vitae 《Automatica》2002,38(9):1565-1573
Analysis of exclusively kinetic two-link underactuated mechanical systems is undertaken in this paper. It is first shown that such systems are not full-state feedback linearizable around any equilibrium point. Also, the equilibrium points for which the system is small-time locally controllable (STLC) is at most a one-dimensional submanifold. A concept less restrictive than STLC, termed the small-time local output controllability (STLOC) is introduced, the satisfaction of which guarantees that a chosen configuration output can be controlled at its desired value. It is shown that the class of systems considered is STLOC, if the inertial coupling between the input and output is nonzero. Also, in such a case, the system is nonminimum phase. An example section illustrates all the results presented. 相似文献
86.
87.
H.A. Barker Author Vitae A.H. Tan Author Vitae K.R. Godfrey Author Vitae 《Automatica》2003,39(1):127-133
Analysis of systems with direction-dependent dynamics is currently limited to cases in which the dynamics in the two directions of the output are first order; results for such systems have been published for both pseudo-random maximum-length binary (MLB) and inverse-repeat maximum-length binary (IR-MLB) inputs. These relatively limited analytical results make it useful to examine alternative ways of modelling such systems and in this paper, Wiener models are considered for this purpose. Methods for optimising the Wiener model parameters by matching the system and model cross-correlation functions, outputs, and discrete Fourier transforms of the outputs are considered, and the results are compared. These methods are also applied to a first-order direction-dependent system with a maximum-length ternary (MLT) input, for which no analytical results are currently available, and to a second-order system with an IR-MLB input. 相似文献
88.
Terry WindeattAuthor Vitae 《Pattern recognition》2003,36(12):2743-2756
Various measures, such as Margin and Bias/Variance, have been proposed with the aim of gaining a better understanding of why Multiple Classifier Systems (MCS) perform as well as they do. While these measures provide different perspectives for MCS analysis, it is not clear how to use them for MCS design. In this paper a different measure based on a spectral representation is proposed for two-class problems. It incorporates terms representing positive and negative correlation of pairs of training patterns with respect to class labels. Experiments employing MLP base classifiers, in which parameters are fixed but systematically varied, demonstrate the sensitivity of the proposed measure to base classifier complexity. 相似文献
89.
Beutler Larry E.; Williams Rebecca E.; Wakefield Phylis J.; Entwistle Stephanie R. 《Canadian Metallurgical Quarterly》1995,50(12):984
Literature suggests that a complex and often hostile relationship exists between the science and practice of clinical psychology. Contributors to this conflict of viewpoints are reconsidered within the proposition that there are different roads to discovery and that there may be good reasons to keep the science and practice of clinical psychology somewhat separate. Results of a national survey of 325 psychologists are reviewed that support the view that psychological practitioners value research and consider their practices to be augmented by scientific findings. However, they are in need of vehicles of communication that will help them translate scientific findings into practice. Results suggest that practitioners do more to understand scientific findings than scientists do to understand the problems that face clinical practitioners. Ways to facilitate communication between and among these groups are considered. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
90.
The authors report on the fabrication and characteristics of a unipolar, three-terminal, resonant-tunneling transistor. The operating principle of this new transistor is based on the fact that the quantum mechanical resonant-tunneling probability of hot electrons between the emitter and the collector is switched almost completely on and off, when either the base or the collector bias is swept. The emitter injects hot electrons to the second lowest subband of a thin (100 Å in this work) GaAs quantum well. Subsequently, the hot electrons will either resonantly tunnel to the collector, or relax to the lowest subband and contribute to the base current. As a result of resonant transmission, at 77 K the current-voltage characteristics of the transistor display negative differential resistance with extremely large (4691) peak-to-valley ratio. Furthermore, when biased near resonance, a maximum DC current gain of ~1.2 and a maximum AC current gain of ~11.9 were observed. The first use of a new `tunneling-in and tunneling-out' scheme in contacting a thin quantum well is also demonstrated 相似文献