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21.
Pulsed light for the inactivation of fungal biofilms of clinically important pathogenic Candida species 下载免费PDF全文
Microorganisms are naturally found as biofilm communities more than planktonic free‐floating cells; however, planktonic culture remains the current model for microbiological studies, such as disinfection techniques. The presence of fungal biofilms in the clinical setting has a negative impact on patient mortality, as Candida biofilms have proved to be resistant to biocides in numerous in vitro studies; however, there is limited information on the effect of pulsed light on sessile communities. Here we report on the use of pulsed UV light for the effective inactivation of clinically relevant Candida species. Fungal biofilms were grown by use of a CDC reactor on clinically relevant surfaces. Following a maximal 72 h formation period, the densely populated biofilms were exposed to pulsed light at varying fluences to determine biofilm sensitivity to pulsed‐light inactivation. The results were then compared to planktonic cell inactivation. High levels of inactivation of C. albicans and C. parapsilosis biofilms were achieved with pulsed light for both 48 and 72 h biofilm structures. The findings suggest that pulsed light has the potential to provide a means of surface decontamination, subsequently reducing the risk of infection to patients. The research described herein deals with an important aspect of disease prevention and public health. 相似文献
22.
Wan Abd Al Qadr Imad Wan-Mohtar Zul Ilham Adi Ainurzaman Jamaludin Neil Rowan 《International journal of molecular sciences》2021,22(4)
Natural mycelial exopolysaccharide (EPS) and endopolysaccharide (ENS) extracted from bioreactor-cultivated European Ganoderma applanatum mushrooms are of potential high commercial value for both food and adjacent biopharmaceutical industries. In order to evaluate their potential toxicity for aquaculture application, both EPS (0.01–10 mg/mL) and ENS (0.01–10 mg/mL) extracts were tested for Zebrafish Embryo Toxicity (ZFET); early development effects on Zebrafish Embryos (ZE) were also analyzed between 24 and 120 h post-fertilization (HPF). Both EPS and ENS are considered non-toxic with LC50 of 1.41 mg/mL and 0.87 mg/mL respectively. Both EPS and ENS did not delay hatching and teratogenic defect towards ZE with <1.0 mg/mL, respectively. No significant changes in the ZE heart rate were detected following treatment with the two compounds tested (EPS: 0.01–10 mg/mL: 176.44 ± 0.77 beats/min and ENS: 0.01–10 mg/mL: 148.44 ± 17.75 beats/min) compared to normal ZE (120–180 beats/min). These initial findings support future pre-clinical trials in adult fish models with view to safely using EPS and ENS as potential feed supplements for supplements for development of the aquaculture industry. 相似文献
23.
Simon J. Hook Jane E. Dmochowski Lawrence C. Rowan Joann M. Stock 《Remote sensing of environment》2005,95(3):273-289
Remotely sensed multispectral thermal infrared (8-13 μm) images are increasingly being used to map variations in surface silicate mineralogy. These studies utilize the shift to longer wavelengths in the main spectral feature in minerals in this wavelength region (reststrahlen band) as the mineralogy changes from felsic to mafic. An approach is described for determining the amount of this shift and then using the shift with a reference curve, derived from laboratory data, to remotely determine the weight percent SiO2 of the surface. The approach has broad applicability to many study areas and can also be fine-tuned to give greater accuracy in a particular study area if field samples are available. The approach was assessed using airborne multispectral thermal infrared images from the Hiller Mountains, Nevada, USA and the Tres Virgenes-La Reforma, Baja California Sur, Mexico. Results indicate the general approach slightly overestimates the weight percent SiO2 of low silica rocks (e.g. basalt) and underestimates the weight percent SiO2 of high silica rocks (e.g. granite). Fine tuning the general approach with measurements from field samples provided good results for both areas with errors in the recovered weight percent SiO2 of a few percent. The map units identified by these techniques and traditional mapping at the Hiller Mountains demonstrate the continuity of the crystalline rocks from the Hiller Mountains southward to the White Hills supporting the idea that these ranges represent an essentially continuous footwall block below a regional detachment. Results from the Baja California data verify the most recent volcanism to be basaltic-andesite. 相似文献
24.
The quantum dot solar concentrator (QDSC) is a novel non-tracking solar concentrator comprising quantum dots (QDs) seeded in materials such as plastics and glasses, that concentrates both direct and diffuse solar energy on attached photovoltaic cells. Spectroscopic measurements have been undertaken for a range of different quantum dot (QD) types and transparent host materials. High transparency in the matrix material and QDs with high quantum efficiency are essential for an efficient QDSC. An optimum matrix material for a QDSC has been determined based on absorption characteristics and an optimum commercially available QD type has been chosen using steady-state absorption, photoluminescence and photoluminescence excitation spectroscopy of QDs in solution and solid matrices. 相似文献
25.
Johannes A. A. W. Elemans Vincent F. Slagt Alan E. Rowan Roeland J. M. Nolte 《Israel journal of chemistry》2005,45(3):271-279
The development of self-assembling catalysts, although an often-tried approach, has achieved little success so far. Toward the construction of substrate selective catalysts, we have self-assembled a porphyrin cage based upon the recognition of the templates meso-dipyridyl ( DPyP ) or meso-tetrapyridyl porphyrin ( TPyP ) by glycoluril-based receptors that are functionalized with two pendant zinc porphyrins. 相似文献
26.
The harmonic balance method is a technique for the numerical solution of nonlinear analog circuits operating in a periodic, or quasi-periodic, steady-state regime. The method can be used to efficiently derive the continuous-wave response of numerous nonlinear microwave components including amplifiers, mixers, and oscillators. Its efficiency derives from imposing a predetermined steady-state form for the circuit response onto the nonlinear equations representing the network, and solving for the set of unknown coefficients in the response equation. Its attractiveness for nonlinear microwave applications results from its speed and ability to simply represent the dispersive, distributed elements that are common at high frequencies. The last decade has seen the development and application of harmonic balance techniques to model analog circuits, particularly microwave circuits. The first part of this article reviewed the fundamental achievements made during this time. In this part, the extension of the method to quasi-periodic regimes, optimization analysis, oscillator analysis, studies of various convergence strategies, and practical applications are discussed. A critical assessment of the various types of harmonic balance techniques is given. Examples of designs which have been modeled using the harmonic balance technique and built both in hybrid and MMIC form are presented. 相似文献
27.
Rowan Dobson Patrick Folly Alexandre Sarbach Romuald Van Riet Bertrand Roduit Jörgen Sandström Erik Tunestål Amy Carlström Alain Dejeaifve 《Propellants, Explosives, Pyrotechnics》2024,49(4):e202300303
Stabilisers are an integral part of a propellant composition. They are essential for ensuring the chemical stability of nitrate-ester based propellants, preventing oxidation, chain scission and uncontrolled heat generation. Progress is being made, especially in Europe, with REACh legislation (1907/2006) to use more environmentally-friendly compounds. New stabilisers with a greener environmental profile and producing less toxic daughter products have been investigated by several groups, and several compounds demonstrated even better performances – more chemical stability and therefore longer shelf lives than formulations with the currently-used stabilisers. Additionally, the good efficiencies and lower toxicities of the daughter products of the “green” stabilisers are also evaluated. This paper is a continuation of our previous work and demonstrates that for industrially-manufactured propellants, that the tests performed by four different entities validate the stability and compatibility of the propellants, investigated previously on a laboratory scale, providing the community with safer formulations for the environment and health. 相似文献
28.
Heart rate (HR) and heart rate variability (HRV) are risk markers in cardiac disease. HRV is also an index of the sympathovagal modulation of heart rate. Their relations have been rarely analyzed. We aimed to study such relations in normal adult conscious rats by using a novel bradycardic agent, a sinus node inhibitor, S-16257. Placebo-drug crossover designs were used while monitoring HR with telemetry and analyzing HRV in both time and frequency domains. S-16257 (2 mg/kg; n = 10) decreased HR by 29% and markedly increased HRV in parallel. By using various combinations of S-16257, atropine (2 mg/kg), and propranolol (4 mg/kg), a positive relation was shown between RR interval and various indexes of HRV: the slower the HR, the greater the HRV. Nevertheless, there is one exception to this correlation. When S-16257 was associated with both atropine and propranolol, the deep bradycardia was accompanied by a reduction of HRV, which indicates that the physiologic negative correlation between HR and HRV is not an intrinsic property of the pacemaker but is highly dependent on the two components of the autonomic system. 相似文献
29.
Detailed models are presented for the stationary and synchronous sine-triangle current regulators. Analytical and test results are compared for purposes of model verification and regulator evaluation. The results demonstrate the limitations of the two most often used current regulators and the robustness of the synchronous current regulator. The stationary sine-triangle and hysteretic current regulators are shown to have steady-state characteristics that depend on slip, operating frequency, and motor impedance. In contrast the synchronous regulator, because it lacks these dependencies, exhibits ideal steady-state current regulator characteristics without sacrificing bandwidth. Moreover, the complexities traditionally associated with the synchronous regulator are overcome with a simple equivalent implementation. 相似文献
30.
Kerkman R.J. Seibel B.J. Rowan T.M. Schlegel D.W. 《Industry Applications, IEEE Transactions on》1996,32(3):585-593
The effect of stator resistance on AC drive performance is analyzed for flux vector and indirect field-oriented controllers. A new technique-the back electromagnetic force (BEMF) detector-for reducing the adverse effects of stator resistance on field-oriented control is presented and evaluated through simulation and experimental results. The BEMF detector is shown to reduce the impact of the stator resistance variations and also provide an estimate of the stator resistance. The detector is compatible with most control strategies and with or without position feedback 相似文献