PRP4 Promotes Skin Cancer by Inhibiting Production of Melanin,Blocking Influx of Extracellular Calcium,and Remodeling Cell Actin Cytoskeleton

Pre-mRNA processing factor 4B (PRP4) has previously been shown to induce epithelial-mesenchymal transition (EMT) and drug resistance in cancer cell lines. As melanin plays an important photoprotective role in the risk of sun-induced skin cancers, we have investigated whether PRP4 can induce drug resistance and regulate melanin biosynthesis in a murine melanoma (B16F10) cell line. Cells were incubated with a crucial melanogenesis stimulator, alpha-melanocyte-stimulating hormone, followed by transfection with PRP4. This resulted in the inhibition of the production of melanin via the downregulation of adenylyl cyclase-cyclic adenosine 3′,5′-monophosphate (AC)–(cAMP)–tyrosinase synthesis signaling pathway. Inhibition of melanin production by PRP4 leads to the promotion of carcinogenesis and induced drug resistance in B16F10 cells. Additionally, PRP4 overexpression upregulated the expression of β-arrestin 1 and desensitized the extracellular calcium-sensing receptor (CaSR), which in turn, inhibited the influx of extracellular Ca²⁺ ions. The decreased influx of Ca²⁺ was confirmed by a decreased expression level of calmodulin. We have demonstrated that transient receptor potential cation channel subfamily C member 1 was involved in the influx of CaSR-induced Ca²⁺ via a decreasing level of its expression. Furthermore, PRP4 overexpression downregulated the expression of AC, decreased the synthesis of cAMP, and modulated the actin cytoskeleton by inhibiting the expression of Ras homolog family member A (RhoA). Our investigation suggests that PRP4 inhibits the production of melanin in B16F10 cells, blocks the influx of Ca²⁺ through desensitization of CaSR, and modulates the actin cytoskeleton through downregulating the AC–cAMP pathway; taken together, these observations collectively lead to the promotion of skin carcinogenesis.     

A numerical study on heat transfer enhancement and design of a heat exchanger with porous media in continuous hydrothermal flow synthesis system

The aim of this study is to use a new configuration of porous media in a heat exchanger in continuous hydrothermal flow synthesis (CHFS) system to enhance the heat transfer and minimize the required length of the heat exchanger.For this purpose,numerous numerical simulations are performed to investigate performance of the system with porons media.First,the numerical simulation for the heat exchanger in CHFS system is validated by experimental data.Then,porous media is added to the system and six different thicknesses for the porous media are examined to obtain the optimum thickness,based on the minimum required length of the heat exchanger.Finally,by changing the flow rate and inlet temperature of the product as well as the cooling water flow rate,the minimum required length of the heat exchanger with porous media for various inlet conditions is assessed.The investigations indicate that using porous media with the proper thickness in the heat exchanger increases the cooling rate of the product by almost 40％and reduces the required length of the heat exchanger by approximately 35％.The results also illustrate that the most proper thickness of the porous media is approximately equal to 90％ of the product tube's thickness.Results of this study lead to design a porous heat exchanger in CHFS system for various inlet conditions.     

A self-tuning fuzzy PI controller for TCSC to improve power system stability

In this paper, a self-tuning fuzzy PI controller (STFPIC) is proposed for thyristor-controlled series capacitor (TCSC) to improve power system dynamic performance. In a STFPIC controller, the output-scaling factor is adjusted on-line by an updating factor (α). The value of α is determined from a fuzzy rule-base defined on error (e) and change of error (Δe) of the controlled variable. The proposed self-tuning controller is designed using a very simple control rule-base and the most natural and unbiased membership functions (MFs) (symmetric triangles with equal base and 50% overlap with neighboring MFs). The comparative performances of the proposed STFPIC and the standard fuzzy PI controller (FPIC) have been investigated on two multi-machine power systems (namely, 4 machine, 2 area system and 10 machine 39 bus system) through detailed non-linear simulation studies using MATLAB/SIMULINK. From the simulation studies it has been found out that for damping oscillations, the performance of the proposed STFPIC is better than that obtained by the standard FPIC. Moreover, the proposed STFPIC as well as the FPIC have been found to be quite effective in damping oscillations over a wide range of operating conditions and are quite effective in enhancing the power carrying capability of the power system significantly.     

Adaptive synchronization of master–slave systems with mixed neutral and discrete time‐delays and nonlinear perturbations

This paper investigates the delay‐dependent adaptive synchronization problem of the master and slave structure of linear systems with both constant neutral and time‐varying discrete time‐delays and nonlinear perturbations based on the Barbalat lemma and matching conditions. An adaption law which includes the master‐slave parameters is obtained by using the Lyapunov functional method and inequality techniques to synchronize the master‐slave systems without the knowledge of upper bounds of perturbation terms. Particularly, it is shown that the synchronization speed can be controlled by adjusting the update gain of the synchronization signal. A numerical example has been given to show the effectiveness of the method. Copyright © 2011 John Wiley and Sons Asia Pte Ltd and Chinese Automatic Control Society     

圆形封闭水箱非稳态数值模拟

采用数值方法对圆形封闭水箱内放置两根加热管的二维流动传热进行了非稳态数值模拟,探讨了Ra数从104到107四种情况和封闭水箱的加热管不同摆放位置下的流动和换热;对流场和温度场进行了分析,讨论了不同情况下加热管平均努赛尔数(Nuavg)的变化情况。数值模拟结果表明,随着Ra数增大水箱内的流动表现出了很大变化,决定了传热是导热或者是对流;两个水平加热管在水箱存在最佳的放置距离,这样可以使自然对流情况下的换热达到最好。     

Generative regular-freeform surface recognition for generating material removal volume from stock model

The present paper illustrates the development of generative surface recognition for regular and freeform. To obtain the final form of product, material removal volume generation from a stock model is also discussed. Only a few studies integrated the regular and freeform surfaces to provide a comprehensive definition of surface recognition as well as for the volumetric estimation of removal material in finishing and roughing operations. The current research deploys a comprehensive surface recognition approach that can recognise both regular and freeform surfaces based on the geometry as well as loop entity of a face. In contrast to the regular surface that can be categorised into a particular group of geometrical shape, such as cylindrical shape, the proposed approach enables the recognition of a freeform surface that cannot be defined as a generic geometrical shape. In addition, the new method also simplifies the existing surface recognition for regular surfaces. The material removal volumes created consist of machining volumes for finishing and roughing operations needed to be machined to obtain the final form of the product. The present research provides a unique user customisation feature that enables user to specify the volumetric thickness for material removal volume in the finishing operation as well as the size for the stock model. These estimated volumes are prepared for subsequent manufacturing applications, such as sequencing of machining operation.     

Robust image watermarking scheme using bit-plane of hadamard coefficients

Multimedia Tools and Applications - Nowadays, due to widespread usage of the Internet, digital contents are distributed quickly and inexpensively throughout the world. Watermarking techniques can...     

Design and analysis of a fault tolerant hybrid mobile scheme

Mobile computing systems provide users with access to information regardless of their geographical location. In these systems, Mobile Support Stations (MSSs) play the role of providing reliable and uninterrupted communication and computing facilities to mobile hosts. The failure of a MSS can cause interruption of services provided by the mobile system. Two basic schemes for tolerating the failure of MSSs exist in the literature. The first scheme is based on the principle of checkpointing used in distributed systems. The second scheme is based on state information replication of mobile hosts in a number of secondary support stations. Depending on the replication scheme used, the second approach is further classified as a pessimistic or an optimistic technique. In this paper, we propose a hybrid scheme which combines the pessimistic and the optimistic replication schemes. In the proposed scheme, an attempt is made to strike a balance between the long delay caused by the pessimistic and the high memory requirements of the optimistic schemes. In order to find the best ratio between the number of pessimistic to the number of optimistic secondary stations in the proposed scheme, we used fuzzy logic. We also used simulation to compare the performance of the proposed scheme with those of the optimistic and the pessimistic schemes. Simulation results showed that the proposed scheme performs better than either schemes in terms of delay and memory requirements.     

Synthesis and characterization of novel curcumin based polyurethanes varying diisocyanates structure

In this research work, novel polyurethanes (PUs) based on blends of curcumin/1,4-butane diol (BDO) by varying the structure of diisocyanates were prepared following step growth polymerization. Structural study of blends and various diisocyanates based PU through Fourier Transform Infrared (FTIR) spectroscopy confirmed the incorporation of curcumin into the backbone of the PU. The scanning electron microscopic (SEM) study confirmed the well dispersion of incorporated curcumin and homogeneity of surface of synthesized samples. The SEM results also indicated that surface morphology of synthesized samples much dependent on diisocynates structure. Moreover SEM images inferred that phase separation is more pronounced in aromatic diisocyanate based PU. The anti-bacterial and anti-fungal tests were performed against different strains in order to determine the biocompatibility of the curcumin based PU. The antimicrobial activity results revealed that the material having aromatic diisocyanate are more biocompatible than the aliphatic diisocyanates in the PU structure. On the whole, this work is actually a step towards the generation of novel biocompatible materials preferably useful for biomedical applications.     

Measurement of in-plane effective thermal conductivity in PEM fuel cell diffusion media

Gas diffusion media used in polymer electrolyte membrane (PEM) fuel cells are highly anisotropic with significantly different transport property values in the through- and in-plane directions. In this study, experimental measurements of the in-plane effective thermal conductivity k for gas diffusion media used in PEM fuel cells have been carried out using a parallel thermal conductance technique. Conductivity values are measured at a mean sample temperature of 70 °C for six different material types and two different orientations in order to quantify the effect of PTFE content on thermal conductivity and to reveal any anisotropic behavior. The results vary from a minimum of k = 3.54 W/(m °C) to a maximum value of 15.1 W/(m °C) for various samples and configurations tested in this study, with an uncertainty between 1% and 2% for all the cases investigated.