VAE/MPM/GA Technique for DoA Estimation Using Optimized Antenna Arrays

Many algorithms have been proposed to estimate the direction of arrival for the targets, but through using a large number of snapshots. In real time applications such as automotive radar, this is unacceptable as it causes delay and heavy processing. Instead, if only a small number of snapshots or, optimally, a single snapshot is available for DoA estimation, it will be fast and efficient. Single snapshot algorithms have a drawback as they require a large number of antenna elements, which considered a limiting factor. In this paper, a single snapshot DoA estimation technique is introduced by using optimized antenna arrays. The proposed algorithm is based on utilization of virtual array extension, matrix pencil method, and the genetic algorithm. The use of virtual array extension greatly improves the MPM performance. Furthermore, it exhibits a high DoA estimation accuracy by using a reduced number of antenna elements. The genetic algorithm is employed to determine the minimum number of antenna elements, which are required to estimate the DoAs with minimal root mean square error.

     

Enhancement of Bioactive Compounds and Antioxidant Activities of Olive (<Emphasis Type="Italic">Olea europaea</Emphasis> L.) Leaf Extract by Instant Controlled Pressure Drop

In this work, the effect of Détente Instantanée Contrôlée (DIC) (French for instant controlled pressure drop) on the total polyphenol, flavonoids, α-tocopherol contents, and antioxidant activities of olive leaves was studied. Olive leaf extracts were pre-treated at one cycle DIC under 0.1 MPa pressure for 11 s and followed by an extraction with 95% ethanol at 55 °C during 3 h. The phenolic compounds, flavonoïds, oleuropein, and α-tocopherol contents were determined, showing 66.63 mg gallic acid equivalent (GAE)/g db, 12 mg catechin equivalent (CE)/g db, 43.9 mg/g db, and 0.15 mg/g db for the untreated leaves against 239.37 mg GAE/g db, 28 mg CE/g db, 70.3 mg/g db, and 0.59 mg/g db for DIC-treated leaves, respectively. Therefore, DIC allows more availability of bioactive compounds contributing to a high antiradical activity (DPPH) compared to a synthetic antioxidant butylated hydroxytoluene (BHT). Both extracts showed a total antioxidant capacity (method of phosphomolybdenum) greater than that of the standard BHT. Likewise, both extracts have a reducing power (FRAP test) significant concentration-dependent. The DIC-treated leaves showed a higher antioxidant capacity compared to that of untreated leaves. Thus, DIC could be an effective treatment to promote the extraction of bioactive molecules of high antioxidant activities from olive leaves.     

Solid-support sample loading for DNA sequencing

We present a new method for simplified low-quantity DNA loading onto microelectrophoresis devices. The method is based on combined solid-phase extraction, purification, and transport of DNA reversibly bound on paramagnetic microspheres. DNA is adsorbed onto the microspheres, captured with a magnetized permalloy wire, and then directly injected as a highly focused sample plug into the separation channel. This method circumvents both the minimum volume requirement of pipettors (since only solid beads are transported) and the timing complications of double-T microfluidic injection. Injections from Sanger samples of <100 pg total suspended weight match the signal strength of our previous conventional injections at >10-times the starting DNA sample. Sequencing traces show a resolution that matches or exceeds double-T injections. A kinetic model reproduces the time-dependence of the injection signals and proves that total nonidealities in the method produce injection-broadened plugs of approximately 1-s duration. The method should be broadly extendable to DNA and protein separations in both microdevice and capillary electrophoresis.     

Engineering of Micro‐ and Nanostructured Surfaces with Anisotropic Geometries and Properties

Widespread approaches to fabricate surfaces with robust micro‐ and nanostructured topographies have been stimulated by opportunities to enhance interface performance by combining physical and chemical effects. In particular, arrays of asymmetric surface features, such as arrays of grooves, inclined pillars, and helical protrusions, have been shown to impart unique anisotropy in properties including wetting, adhesion, thermal and/or electrical conductivity, optical activity, and capability to direct cell growth. These properties are of wide interest for applications including energy conversion, microelectronics, chemical and biological sensing, and bioengineering. However, fabrication of asymmetric surface features often pushes the limits of traditional etching and deposition techniques, making it challenging to produce the desired surfaces in a scalable and cost‐effective manner. We review and classify approaches to fabricate arrays of asymmetric 2D and 3D surface features, in polymers, metals, and ceramics. Analytical and empirical relationships among geometries, materials, and surface properties are discussed, especially in the context of the applications mentioned above. Further, opportunities for new fabrication methods that combine lithography with principles of self‐assembly are identified, aiming to establish design principles for fabrication of arbitrary 3D surface textures over large areas.     

Effect of sodium chloride and pH on the rennet coagulation and gel firmness

The effect of sodium chloride concentration and pH of milk at renneting on the rennet clotting time (RCT) and gel firmness was studied. The results showed that rennet clotting activity and gel firmness decreased with increasing NaCl concentration in milk. The RCT increased as the pH of the salted milk decreased below 6.4. Milk containing 5 and 10 g NaCl/100 g did not coagulate at pH 5.0. The gel firmness increased with decreasing milk pH to 6.2 for unsalted milk. However, the firmness decreased as the pH of milk decreased below 6.2. In case of using salted milk, the firmness decreased as the pH at renneting dropped below 6.4.     

Combining in-situ proteolysis and microseed matrix screening to promote crystallization of PrPc-nanobody complexes

Prion proteins (PrPs) are difficult to crystallize, probably due to their inherent flexibility. Several PrPs structures have been solved by nuclear magnetic resonance (NMR) techniques; however, only three structures were solved by X-ray crystallography. Here we combined in-situ proteolysis with automated microseed matrix screening (MMS) to crystallize two different PrP(C)-nanobody (Nb) complexes. Nanobodies are single-domain antibodies derived from heavy-chain-only antibodies of camelids. Initial crystallization screening conditions using in-situ proteolysis of mouse prion (23-230) in complex with a nanobody (Nb_PrP_01) gave thin needle aggregates, which were of poor diffraction quality. Next, we used these microcrystals as nucleants for automated MMS. Good-quality crystals were obtained from mouse PrP (89-230)/Nb_PrP_01, belonged to the monoclinic space group P 1 21 1, with unit-cell parameters a = 59.13, b = 63.80, c = 69.79 ?, β = 101.96° and diffracted to 2.1 ? resolution using synchrotron radiation. Human PrP (90-231)/Nb_PrP_01 crystals belonged to the monoclinic space group C2, with unit-cell parameters a = 131.86, b = 45.78, c = 45.09 ?, β = 96.23° and diffracted to 1.5 ? resolution. This combined strategy benefits from the power of the MMS technique without suffering from the drawbacks of the in-situ proteolysis. It proved to be a successful strategy to crystallize PrP-nanobodies complexes and could be exploited for the crystallization of other difficult antigen-antibody complexes.     

A Novel Formulation for Mebeverine Hydrochloride

The antispasmodic drug mebeverine hydrochloride was formulated into a film-forming gel to be used as a topical local anesthetic. A mixture of cellulose derivatives was used as a base. Additives were used to enhance the release as well as the residence time. Formulations were characterized in terms of drug release, mucoadhesion and rheology. Clinically, the selected formula has shown faster onset (p?=?0.0156), longer duration (p?=?0.0313), better film residence (p?=?0.0313), and no foreign body sensation (p?=?0.0313) in comparison to Solcoseryl® dental paste. Histopathological examination showed no change in inflammatory cells count, concluding that this topical anesthetic is efficacious and safe orally.     

Numerical investigations on dynamic performance of stiffened box steel bridge piers

The aim of this paper is to carry out extensive numerical investigations about the effect of various structural parameters on the dynamic performance of stiffened box steel bridge pier under a strong earthquake ground motion. The considered structural parameters are the local slenderness ratios of stiffener and stiffened wall as well as the global slenderness ratio of the pier. In the present study, all investigated piers are made of high tensile steel with high yield ratio. The non-linear time history analyses are carried out using in-house Finite Element Program DYNAPSS, recently developed by the first author. This program is verified by comparing its results with the results obtained by the general FE program. In this program, the geometrical nonlinearity is considered on the bases of Total Lagrangian formulation, while the non-linearity of structural steel material is considered through implementation of modified multisurface cyclic plasticity model by which real characteristics of high tensile steel material such as, Plateau, Massing type of Bauschinger??s effect, disappearing of Plateau and cyclic strain hardening, are accurately modeled. The results are closely examined in respect with the damage index criterion. From this study, it is found that the increase in slenderness ratios of stiffener does not only increase the damage index, but also may lead to the full collapse. Furthermore, the damage index increases with the increase of local slenderness ratio of stiffened wall and the global slenderness ratio. Also, it is found that when the loacal buckling is not the dominant eigen mode and the pier behave as a single degree of freedom, the natural period has destructive effects when it is close to predominant period of the earthquake. Finally, contour plots of damage index values are presented. These contour plots may be greatly useful for the design of stiffened box steel bridge piers, where, critical values of structural parameters corresponding to certain value of damage index could be determined.     

Parallel implementations of the trace minimization scheme TraceMIN for the sparse symmetric eigenvalue problem

Eigenvalue problems arise in many computational science and engineering applications: in structural mechanics, nanoelectronics, and Google’s PageRank link analysis, for example. Often, the large size of these eigenvalue problems requires the development of eigensolvers that scale well on parallel computing platforms. In this paper, we compare the effectiveness and robustness of our eigensolver for the symmetric generalized eigenvalue problem, the trace minimization scheme TraceMIN–developed in the early 1980s–against today’s well-known sparse eigensolvers including: the LOBPCG and block Krylov–Schur implementations in Trilinos; ARPACK; and several methods in the PRIMME package such as the Jacobi–Davidson one. In addition, we demonstrate the parallel scalability of two variants of TraceMIN on multicore nodes as well as on large clusters of such nodes. Our results show that TraceMIN is more robust and has higher parallel scalability than the above-mentioned competing eigensolvers.     

A projection method for solving nonsymmetric linear systems on multiprocessors

We consider the iterative solution of large sparse linear systems of equations arising from elliptic and parabolic partial differential equations in two or three space dimensions. Specifically, we focus our attention on nonsymmetric systems of equations whose eigenvalues lie on both sides of the imaginary axis, or whose symmetric part is not positive definite. This system of equation is solved using a block Kaczmarz projection method with conjugate gradient acceleration. The algorithm has been designed with special emphasis on its suitability for multiprocessors. In the first part of the paper, we study the numerical properties of the algorithm and compare its performance with other algorithms such as the conjugate gradient method on the normal equations, and conjugate gradient-like schemes such as ORTHOMIN(k), GCR(k) and GMRES(k). We also study the effect of using various preconditioners with these methods. In the second part of the paper, we describe the implementation of our algorithm on the CRAY X-MP/48 multiprocessor, and study its behavior as the number of processors is increased.