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Li Xi Zuoyan Peng Wei Fan Kui Guo Gu Jianmin Muyu Zhao Wu Guoqiang 《Materials Science and Engineering: B》1996,40(2-3):147-152
SrMgxTi1 - xO3 nanocrystals (x = 0.1–0.6) were synthesized by the stearic acid gel method. Powder samples were characterized by X-ray diffraction and X-ray photoelectron (XP) spectroscopy. The results showed that the lattice parameter a and the O 1s XP spectrum changed not only with the Mg content x but also with the grain size d of the samples. The conductivity of a thick film specimen fabricated on an aluminium oxide wafer was investigated in a nitrogen—oxygen atmosphere. 相似文献
24.
Retroviral display of antibody fragments; interdomain spacing strongly influences vector infectivity 总被引:1,自引:0,他引:1
S Ager BH Nilson FJ Morling KW Peng FL Cosset SJ Russell 《Canadian Metallurgical Quarterly》1996,7(17):2157-2164
Five different single-chain antibody fragments (scFv) against human cell-surface antigens were displayed on murine ecotropic retroviral vectors by fusing them to the Moloney SU envelope glycoprotein. The spacing between the scFv and the SU glycoprotein was varied by fusing the scFv to residue +7 or to residue +1 of Moloney SU and by inserting linker sequences of different lengths between the domains. All of the chimeric envelopes were efficiently incorporated into vector particles and could bind to human cells through their displayed antibody fragments, but did not infect them. The spacing between the scFvs and the SU glycoproteins had no significant effect on the efficiency of envelope expression or viral incorporation and did not affect the binding properties of the chimeric envelopes, nor did it influence the efficiency of targeted gene delivery to human cells by scFv-displaying vectors. However, on murine fibroblasts the infectivity of vectors incorporating the chimeric envelopes was strongly influenced by the length of the interdomain spacer. The titers were very low when the single-chain antibodies were fused through a tripeptide linker to SU residue +7 and were greatly enhanced (up to 10(5)-fold) when they were fused to SU residue +1 through a heptapeptide linker. These results point to the importance of steric interactions between the domains of chimeric envelope glycoproteins and may have implications for retroviral vector design for human gene therapy. 相似文献
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We present a method that is useful in the estimate and assessment of heat capacity data. The approach is based on an analysis of the logarithmic average of the phonon frequencies. In this quantity, that may be easily derived from experimental data on the vibrational entropy, the influence of atomic masses can be exactly accounted for even in polyatomic solids. Our method is applied to Li2O, Na2O, K2O, Rb2O, and Cs2O. In particular, literature data for K2O are critically examined. 相似文献
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A conjugative aminoglycoside resistance plasmid pST2 has been isolated from Escherichia coli K-12 14R525, which was mated with a clinical isolate of Salmonella typhimurium S24. A novel resistance gene of aminoglycoside 6'-N-acetyltransferase[AAC(6')] was cloned from plasmid pST2 on a 1,393 kilobase (kb) of SphI-SalI fragment into vector pACYC184 and pUC18. This novel AAC(6') gene in plasmid pST2 acetylated kanamycin, amikacin, dibekacin, tobramycin, gentamicin, netilmicin, and sisomicin. The complete nucleotide sequence of the novel AAC(6') gene and its neighboring sequences were also determined. Minicell experiments detected only one protein of 24.7 kilodaltons (kDa) translated from an open reading frame of the 618 base pairs (bp) gene. 相似文献
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S. T. J. Peng 《Journal of Materials Science》1985,20(6):1920-1928
In a previous paper, a constitutive equation of relaxation behaviour of time-dependent chemically unstable materials has been developed by employing the irreversible thermodynamics of internal variables and Eyring's absolute reaction theory. In that paper, a theoretical expression for the effect of chemical crosslink density,v, on the relaxation rate has been developed. In this paper the creep behaviour of a network polymer undergoing a scission process has been developed. The temperature effect using the WLF equation on the coupled chemomechanical behaviour has also been incorporated into the equation. 相似文献