全文获取类型
收费全文 | 344篇 |
免费 | 19篇 |
国内免费 | 1篇 |
专业分类
化学工业 | 80篇 |
金属工艺 | 10篇 |
机械仪表 | 9篇 |
建筑科学 | 38篇 |
能源动力 | 10篇 |
轻工业 | 55篇 |
无线电 | 16篇 |
一般工业技术 | 65篇 |
冶金工业 | 15篇 |
自动化技术 | 66篇 |
出版年
2024年 | 2篇 |
2023年 | 17篇 |
2022年 | 9篇 |
2021年 | 20篇 |
2020年 | 5篇 |
2019年 | 13篇 |
2018年 | 11篇 |
2017年 | 11篇 |
2016年 | 14篇 |
2015年 | 22篇 |
2014年 | 14篇 |
2013年 | 28篇 |
2012年 | 29篇 |
2011年 | 34篇 |
2010年 | 25篇 |
2009年 | 22篇 |
2008年 | 18篇 |
2007年 | 15篇 |
2006年 | 8篇 |
2005年 | 9篇 |
2004年 | 7篇 |
2003年 | 8篇 |
2002年 | 4篇 |
2001年 | 3篇 |
2000年 | 3篇 |
1999年 | 1篇 |
1998年 | 6篇 |
1996年 | 1篇 |
1995年 | 1篇 |
1992年 | 1篇 |
1987年 | 2篇 |
1984年 | 1篇 |
排序方式: 共有364条查询结果,搜索用时 15 毫秒
61.
Wilhelm S Rosenau F Becker S Buest S Hausmann S Kolmar H Jaeger KE 《Chembiochem : a European journal of chemical biology》2007,8(1):55-60
Lipases are important enzymes in biotechnology. Extracellular bacterial lipases from Pseudomonads and related species require the assistance of specific chaperones, designated "Lif" proteins (lipase specific foldases). Lifs, a unique family of steric chaperones, are anchored to the periplasmic side of the inner membrane where they convert lipases into their active conformation. We have previously shown that the autotransporter protein EstA from P. aeruginosa can be used to direct a variety of proteins to the cell surface of Escherichia coli. Here we demonstrate for the first time the functional cell-surface display of the Lif chaperone and FACS (fluorescence-activated cell sorting)-based analysis of bacterial cells that carried foldase-lipase complexes. The model Lif protein, LipH from P. aeruginosa, was displayed at the surface of E. coli cells. Surface exposed LipH was functional and efficiently refolded chemically denatured lipase. The foldase autodisplay system reported here can be used for a variety of applications including the ultrahigh-throughput screening of large libraries of foldase variants generated by directed evolution. 相似文献
62.
Remediation of groundwater contaminated with MTBE and benzene: the potential of vertical-flow soil filter systems 总被引:1,自引:0,他引:1
van Afferden M Rahman KZ Mosig P De Biase C Thullner M Oswald SE Müller RA 《Water research》2011,45(16):5063-5074
Field investigations on the treatment of MTBE and benzene from contaminated groundwater in pilot or full-scale constructed wetlands are lacking hugely. The aim of this study was to develop a biological treatment technology that can be operated in an economic, reliable and robust mode over a long period of time. Two pilot-scale vertical-flow soil filter eco-technologies, a roughing filter (RF) and a polishing filter (PF) with plants (willows), were operated independently in a single-stage configuration and coupled together in a multi-stage (RF + PF) configuration to investigate the MTBE and benzene removal performances. Both filters were loaded with groundwater from a refinery site contaminated with MTBE and benzene as the main contaminants, with a mean concentration of 2970 ± 816 and 13,966 ± 1998 μg L−1, respectively. Four different hydraulic loading rates (HLRs) with a stepwise increment of 60, 120, 240 and 480 L m−2 d−1 were applied over a period of 388 days in the single-stage operation. At the highest HLR of 480 L m−2 d−1, the mean concentrations of MTBE and benzene were found to be 550 ± 133 and 65 ± 123 μg L−1 in the effluent of the RF. In the effluent of the PF system, respective mean MTBE and benzene concentrations of 49 ± 77 and 0.5 ± 0.2 μg L−1 were obtained, which were well below the relevant MTBE and benzene limit values of 200 and 1 μg L−1 for drinking water quality. But a dynamic fluctuation in the effluent MTBE concentration showed a lack of stability in regards to the increase in the measured values by nearly 10%, which were higher than the limit value. Therefore, both (RF + PF) filters were combined in a multi-stage configuration and the combined system proved to be more stable and effective with a highly efficient reduction of the MTBE and benzene concentrations in the effluent. Nearly 70% of MTBE and 98% of benzene were eliminated from the influent groundwater by the first vertical filter (RF) and the remaining amount was almost completely diminished (∼100% reduction) after passing through the second filter (PF), with a mean MTBE and benzene concentration of 5 ± 10 and 0.6 ± 0.2 μg L−1 in the final effluent. The emission rate of volatile organic compounds mass into the air from the systems was less than 1% of the inflow mass loading rate. The results obtained in this study not only demonstrate the feasibility of vertical-flow soil filter systems for treating groundwater contaminated with MTBE and benzene, but can also be considered a major step forward towards their application under full-scale conditions for commercial purposes in the oil and gas industries. 相似文献
63.
Christoph Thurm Burkhart Schraven Sascha Kahlfuss 《International journal of molecular sciences》2021,22(17)
ATP-binding cassette (ABC) transporters represent a heterogeneous group of ATP-dependent transport proteins, which facilitate the import and/or export of various substrates, including lipids, sugars, amino acids and peptides, ions, and drugs. ABC transporters are involved in a variety of physiological processes in different human tissues. More recent studies have demonstrated that ABC transporters also regulate the development and function of different T cell populations, such as thymocytes, Natural Killer T cells, CD8+ T cells, and CD4+ T helper cells, including regulatory T cells. Here, we review the current knowledge on ABC transporters in these T cell populations by summarizing how ABC transporters regulate the function of the individual cell types and how this affects the immunity to viruses and tumors, and the course of autoimmune diseases. Furthermore, we provide a perspective on how a better understanding of the function of ABC transporters in T cells might provide promising novel avenues for the therapy of autoimmunity and to improve immunity to infection and cancer. 相似文献
64.
Sascha Bach Karsten Thürling Jens‐Peter Majschak 《Packaging Technology and Science》2012,25(4):233-248
The closure process for packaging is a key process. It ensures the protective function of packaging and assures the packaged goods a long life. In this context, efficient and reliable sealing processes are essential for the production of sustainable packages. In this paper, several characteristics of the ultrasonic sealing process will be discussed and accompanied by experimental results. The introduction provides an insight into the ultrasonic sealing process, its heating mechanisms and the process steps. A comparison is made with conduction sealing. Furthermore, basic principles of heating and energy dissipation are related to the influences of the sealing parameters on the seam strength. The experimental studies were carried out on typical packaging films, such as polyamide‐polyethylene laminates. The seam strength in ultrasonic sealing is compared with that in conductive sealing. A lower seam strength was found for ultrasonic sealing in all the tested films. Furthermore, the sealing behaviour of the packaging films contaminated with different kinds of foods was analysed for both sealing methods. Although the ultrasonic sealing method has marginal advantages for bulk materials such as wheat flour, conduction sealing was shown to be better for other products. A comparison of the energy consumption during the ultrasonic and conduction sealing verified the advantages of ultrasonic sealing. In particular for thick packaging films, the amount of energy required for bonding is significantly lower than for conduction sealing. In summary, this paper provides a survey of the characteristics of the ultrasonic sealing method in packaging applications – its advantages and limitations. Copyright © 2011 John Wiley & Sons, Ltd. 相似文献
65.
66.
67.
Conrad Markus; Carreiras Manuel; Tamm Sascha; Jacobs Arthur M. 《Canadian Metallurgical Quarterly》2009,35(2):461
Over the last decade, there has been increasing evidence for syllabic processing during visual word recognition. If syllabic effects prove to be independent from orthographic redundancy, this would seriously challenge the ability of current computational models to account for the processing of polysyllabic words. Three experiments are presented to disentangle effects of the frequency of syllabic units and orthographic segments in lexical decision. In Experiment 1 the authors obtained an inhibitory syllable frequency effect that was unaffected by the presence or absence of a bigram trough at the syllable boundary. In Experiments 2 and 3 an inhibitory effect of initial syllable frequency but a facilitative effect of initial bigram frequency emerged when manipulating 1 of the 2 measures and controlling for the other in Spanish words starting with consonant-vowel syllables. The authors conclude that effects of syllable frequency and letter-cluster frequency are independent and arise at different processing levels of visual word recognition. Results are discussed within the framework of an interactive activation model of visual word recognition. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
68.
69.
Numerical simulation of pile driving in the area of soil resistance of pit walls. In‐situ measurements at deep excavations during the construction show that the installation of buoyancy piles for the pit floor leads to large deformations of the pit walls. Today these additional loads caused by the construction of adjacent piles cannot be predicted and often lead to astonishment. The aim of this article is to present a first numerical model to predict the additional loads for the ground and the pit walls in the near field of driven piles. Regarding additional deformations of a pit wall at the Potsdamer Platz due to vibratory pile driving of buoyancy piles the importance of the numerical investigation is described. Nevertheless in the future considerable efforts are necessary to receive recommendations for the construction of pit walls. 相似文献
70.
Eduardo Weingärtner Sascha Jaumann Friedrich Kuster Konrad Wegener 《The International Journal of Advanced Manufacturing Technology》2010,48(9-12):1001-1007
Traditional multivariate control charts such as Hotelling’s χ 2 and T 2 control charts are designed to monitor vectors of variable quality characteristics. However, in certain situations, data are expressed in linguistic terms and, under these circumstances, variable or attribute multivariate control charts are not suitable choices for monitoring purposes. Fuzzy multivariate control charts such as fuzzy Hotelling’s T 2 could be considered as efficient tools to overcome the problems of linguistic observations. The purpose of this paper is to develop a fuzzy multivariate exponentially weighted moving average (F-MEWMA) control chart. In this paper, multivariate statistical quality control and fuzzy set theory are combined to develop the proposed method. Fuzzy sets and fuzzy logic are powerful mathematical tools for modeling uncertain systems in industry, nature, and humanity. Through a numerical example, the performance of the proposed control chart was compared to the fuzzy Hotelling’s T 2 control chart. Results indicate uniformly superior performance of the F-MEWMA control chart over Hotelling’s T 2 control chart. 相似文献