Ultra Low-Dose Radiation: Stress Responses and Impacts Using Rice as a Grass Model

We report molecular changes in leaves of rice plants (Oryza sativa L. - reference crop plant and grass model) exposed to ultra low-dose ionizing radiation, first using contaminated soil from the exclusion zone around Chernobyl reactor site. Results revealed induction of stress-related marker genes (Northern blot) and secondary metabolites (LC-MS/MS) in irradiated leaf segments over appropriate control. Second, employing the same in vitro model system, we replicated results of the first experiment using in-house fabricated sources of ultra low-dose gamma (γ) rays and selected marker genes by RT-PCR. Results suggest the usefulness of the rice model in studying ultra low-dose radiation response/s.     

Evaluating crack tip stress field in a thin glass plate under thermal load

The stress field around a propagating crack tip in a quenched thin glass plate is discussed through experimental and theoretical analyses. Instantaneous phase-stepping photoelasticity using a CCD camera equipped with a pixelated micro-retarder array is used for measuring the crack tip stress field. From the successive phase maps of principal direction, the position and the velocity of the crack tip are evaluated. On the other hand, the fracture parameters, that is, the stress intensity factors and the T-stress are determined from the phase maps of the retardation. Experimental results obtained for a straight crack show good agreement with those obtained by theory of elasticity. The results also indicate that the direction of the crack propagation arising in the quenching process is not determined by the direction of the maximum principal stress. Furthermore, the results show that the T-stress criterion is inappropriate to evaluate the crack path instability in a quenched thin glass plate.     

FIDAS: Detailed subchannel analysis code based on the three-fluid and three-field model

The Film Dryout Analysis Code in Subchannels, FIDAS, has been developed with the main objective of predicting dryout and post-dryout heat transfer in a channel and in rod bundles. In FIDAS, two-phase flow consisting of continuous liquid film, continuous vapor and entrained droplets is modeled by a three-fluid, three-field representation of 12 field equations, i.e. three continuity, three energy and six momentum equations. FIDAS can predict dryout without any empirical CHF correlations by introducing annular flow modeling and the ‘film dryout criterion’. Experiments on film flow characteristics, subchannel flow and enthalpy distributions, dryout and post-dryout heat transfer in tubes and rod bundles were analyzed to demonstrate the performance of FIDAS. The predictions of FIDAS are in close agreement with the experiments.     

Examination of the dual‐band microwave wireless access system using radio on fiber technique

The Radio on Fiber (ROF) technique has great potential in wireless communication systems. In this paper, we report on a fundamental examination of microwave wireless access systems based on Radio on Fiber. First, we designed and constructed examination systems by applying dual band (2.4 and 5 GHz) multiservice transmission systems and confirmed that there was sufficient performance through a transmission experiment. Then, application of FP‐LD (Fabry Perot Laser Diode) was considered in order to reduce the system cost, and for the most part degradation of the transmission characteristics was not observed when FP‐LD was used. Finally, a BIDI (bidirectional) module which integrated LD and PD within one package was made to further reduce costs. By measuring the transmission performance, applying the BIDI module to ROF systems was shown to be effective. © 2007 Wiley Periodicals, Inc. Electr Eng Jpn, 160(4): 32–40, 2007; Published online in Wiley InterScience ( www.interscience.wiley.com ). DOI 10.1002/eej.20471     

State-of-the-Art Molecular Dynamics Simulation Studies of RNA-Dependent RNA Polymerase of SARS-CoV-2

Molecular dynamics (MD) simulations are powerful theoretical methods that can reveal biomolecular properties, such as structure, fluctuations, and ligand binding, at the level of atomic detail. In this review article, recent MD simulation studies on these biomolecular properties of the RNA-dependent RNA polymerase (RdRp), which is a multidomain protein, of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are presented. Although the tertiary structures of RdRps in SARS-CoV-2 and SARS-CoV are almost identical, the RNA synthesis activity of RdRp of SARS-CoV is higher than SARS-CoV-2. Recent MD simulations observed a difference in the dynamic properties of the two RdRps, which may cause activity differences. RdRp is also a drug target for Coronavirus disease 2019 (COVID-19). Nucleotide analogs, such as remdesivir and favipiravir, are considered to be taken up by RdRp and inhibit RNA replication. Recent MD simulations revealed the recognition mechanism of RdRp for these drug molecules and adenosine triphosphate (ATP). The ligand-recognition ability of RdRp decreases in the order of remdesivir, favipiravir, and ATP. As a typical recognition process, it was found that several lysine residues of RdRp transfer these ligand molecules to the binding site such as a “bucket brigade.” This finding will contribute to understanding the mechanism of the efficient ligand recognition by RdRp. In addition, various simulation studies on the complexes of SARS-CoV-2 RdRp with several nucleotide analogs are reviewed, and the molecular mechanisms by which these compounds inhibit the function of RdRp are discussed. The simulation studies presented in this review will provide useful insights into how nucleotide analogs are recognized by RdRp and inhibit the RNA replication.     

Application study of a high‐temperature superconducting fault current limiter for electric power system

Using high‐temperature superconductors, a superconducting fault current limiter (SFCL) was fabricated and tested. The superconductor and a vacuum interrupter serving as a commutation switch were connected in parallel with a bypass coil. When a fault occurs and excessive current flows, the superconductor is first quenched and the current is transferred to the bypass coil because of the voltage drop of the superconductor. At the same time, since a magnetic field is generated by the current flowing in the bypass coil, the commutation switch is immediately driven by an electromagnetic repulsion plate connected to the driving rod of the vacuum interrupter (VI), and the superconductor is separated from this circuit. Using the test model, we were able to separate the superconductor from the circuit by the movement of the VI within a half current cycle and to transfer all current to the bypass coil. Since the operation of the commutation switch is included in the current limiting operation of this test model, it will be a useful circuit in the development of SFCL in the future. Moreover, since it can make the energy consumption of the superconductor small during the fault state due to the realization of a high‐speed switch with simple composition, the burden on the superconductor is reduced compared with the conventional resistive type of SFCL and it is considered that the flexibility of SFCL design is increased. Cooperation with a circuit breaker was also considered; trial calculations of the parameters and energy of operation were conducted and a discussion of the installation of the SFCL in an electric power system is presented. © 2006 Wiley Periodicals, Inc. Electr Eng Jpn, 155(4): 20–29, 2006; Published online in Wiley InterScience ( www.interscience.wiley.com ). DOI 10.1002/eej.20265     

Preparation and properties of disyndiotactic poly(alkyl crotonate)s

Ethyl, n-propyl, iso-propyl and n-butyl crotonates were polymerized by group transfer polymerization (GTP) using ketene trialkylsilyl acetals as initiators in the presence of mercury (II) iodide as catalyst and iodotriethylsilane as co-catalyst. Predominantly disyndiotactic polymers with number-average molecular weights ranging from 56,000 to 90,000 were obtained almost quantitatively. Thermal and mechanical properties of poly(alkyl crotonate)s, such as glass transition temperature (T_g), refractive index, transmissivity, Izod impact strength, Young's modulus, hardness, tensile strength, tensile elongation at break, gas permeability coefficient and density were measured. All the polycrotonates have 65–90 °C higher T_g than the corresponding polymethacrylates.     

Phase Diagram Mapping of the Fe<Subscript>3</Subscript>Al-Cr-Mo-C Pseudo-Quaternary System at 800 °C Using a New Diffusion-Multiple Technique

A new diffusion-multiple technique was used for mapping the phase diagram in the pseudo-quaternary Fe₃Al-Cr-Mo-C system at 800 °C. The following five carbide phases were formed in an Fe₃Al matrix phase (B2) with composition gradients of Cr, Mo, and C in the diffusion-multiple samples: κ-Fe₃AlC, M₅C, M₆C, Cr₇C₃, and M₂C (M: Mo, Cr, Al, and Fe). It was assumed that B2 phase is in equilibrium with κ, M₅C, M₆C, and Cr₇C₃ but not with M₂C phase at 800 °C. Complex phase equilibria among those phases were efficiently mapped by the diffusion-multiple technique. The results from the technique were consistent with those obtained from the conventional bulk alloy method.