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91.
Contact conductivity detection in poly(methyl methacrylate)-based microfluidic devices for analysis of mono- and polyanionic molecules 总被引:1,自引:0,他引:1
Galloway M Stryjewski W Henry A Ford SM Llopis S McCarley RL Soper SA 《Analytical chemistry》2002,74(10):2407-2415
An on-column contact conductivity detector was developed for the analysis of various mono- and polyanionic compounds separated by electrophoresis chips fabricated in poly(methyl methacrylate) (PMMA) using hot embossing techniques from Ni electroforms. The detector consisted of a pair of Pt wires (127 microm diameter) with an end-to-end spacing of approximately 20 microm and situated within the fluidic channel. The waveform applied to the electrode pair was a bipolar pulse with a frequency of 5.0 kHz and was used to reduce the charging current from measurement so that the current recorded at the end of one pulse is more representative of the solution conductivity. Using the detector, separations of amino acids, peptides, proteins, and oligonucleotides were demonstrated. For the amino acids and peptides, free-solution zone electrophoresis was performed. A calibration plot for the amino acid alanine was found to be linear from approximately 10 to 100 nM in a carrier electrolyte consisting of 10 mM triethylamonium acetate. The concentration detection limit was found to be 8.0 nM, with the corresponding mass detection limit equal to 3.4 amol (injection volume = 425 pL). The protein separations with conductivity detection were performed using MEKC, in which the carrier electrolyte contained the anionic surfactant sodium dodecyl sulfate (SDS) above its cmc. Near baseline resolution was achieved in the PMMA microchip for a solution containing 8 different proteins. In the case of the DNA fragments, capillary electrochromatography was used with a C18-modified PMMA chip and a carrier electrolyte containing an ion-pairing agent. 相似文献
92.
93.
This study evaluated spinal loads associated with lifting and hanging heavy mining cable in a variety of postures. This electrical cable can weigh up to 10 kg per metre and is often lifted in restricted spaces in underground coal mines. Seven male subjects performed eight cable lifting and hanging tasks, while trunk kinematic data and trunk muscle electromyograms (EMGs) were obtained. The eight tasks were combinations of four postures (standing, stooping, kneeling on one knee, or kneeling on both knees) and two levels of cable load (0 N or 100 N load added to the existing cable weight). An EMG-assisted model was used to calculate forces and moments acting on the lumbar spine. A two-way split-plot ANOVA showed that increased load (p < 0.05) and changes in lifting posture (p < 0.05) independently affected trunk muscle recruitment and spinal loading. The increase in cable load resulted in higher EMG activity of all trunk muscles and increased axial and lateral bending moments on the spine (p < 0.05). Changes in posture caused more selective adjustments in muscle recruitment and affected the sagittal plane moment (p < 0.05). Despite the more selective nature of trunk EMG changes due to posture, the magnitude of changes in spinal loading was often quite dramatic. However, average compression values exceeded 3400 N for all cable lifting tasks. 相似文献
94.
Grounded in the diffusion of innovation theory and the technology–organization–environment framework, we develop an integrative model to study the determinants of post-adoption stages of innovation diffusion, using enterprise digital transformation as an example of technology-enabled innovations. We specify four innovation characteristics (relative advantage, compatibility, costs and security concern) and four contextual factors (technology competence, organization size, competitive pressure and partner readiness) as determinants of post-adoption usage, and postulate usage as an intermediate link to impact on firm performance. We test the proposed model using a dataset of 1415 companies from six European countries. We find that the innovation needs to be used extensively in value-chain activities before its impact can be realized. Among the innovation characteristics, we find that compatibility is the strongest driver, and security concern outweighs cost as a usage inhibitor. Among the contextual variables, technology competence, partner readiness and competitive pressure significantly drive e-business usage, and the structural inertia of large firms tends to slow down its penetration. Collectively, these results indicate that innovation diffusion can be better understood by including both innovation characteristics and contextual factors, whereas earlier literature has traditionally treated the two separately. Finally, we evaluate an international dimension among European countries and tease out important boundary conditions that would not have been evident in a single-country dataset. Our results show that careful attention must be paid to the economic and regulatory factors that may result in uneven innovation diffusion even among developed European countries. 相似文献
95.
An improved in-situ bio-optical data set for ocean color algorithm development and satellite data product validation 总被引:1,自引:0,他引:1
Global satellite ocean color instruments provide the scientific community a high-resolution means of studying the marine biosphere. Satellite data product validation and algorithm development activities both require the substantial accumulation of high-quality in-situ observations. The NASA Ocean Biology Processing Group maintains a local repository of in-situ marine bio-optical data, the SeaWiFS Bio-optical Archive and Storage System (SeaBASS), to facilitate their ocean color satellite validation analyses. Data were acquired from SeaBASS and used to compile a large set of coincident radiometric observations and phytoplankton pigment concentrations for use in bio-optical algorithm development. This new data set, the NASA bio-Optical Marine Algorithm Data set (NOMAD), includes over 3400 stations of spectral water-leaving radiances, surface irradiances, and diffuse downwelling attenuation coefficients, encompassing chlorophyll a concentrations ranging from 0.012 to 72.12 mg m− 3. Metadata, such as the date, time, and location of data collection, and ancillary data, including sea surface temperatures and water depths, accompany each record. This paper describes the assembly and evaluation of NOMAD, and further illustrates the broad geophysical range of stations incorporated into NOMAD. 相似文献
96.
Jim Grundy Tom Melham Sava Krsti Sean McLaughlin 《Electronic Notes in Theoretical Computer Science》2006,144(2):15
Effective formal verification tools require that robust implementations of automatic procedures for first-order logic and satisfiability modulo theories be integrated into expressive interactive frameworks for logical deduction, such as higher-order logic theorem provers. This paper states some pragmatic requirements for implementations of decision procedures that make them well-suited to integration into such frameworks. The aim is to open a dialogue with the designers of decision procedure software that will lead to greater and easier uptake of their implementations by verification users. 相似文献
97.
JA Beatty JJ Callanan A Terry O Jarrett JC Neil 《Canadian Metallurgical Quarterly》1998,72(1):767-771
We describe the histopathological, immunohistochemical, and molecular characterization of a lymphoma arising in a 7-year-old cat following experimental infection with feline immunodeficiency virus (FIV). The tumor was high grade and of B-cell lineage. The transformed cell had an immature phenotype (CD79a+, CD79b-, CD21-, immunoglobulin heavy and light chain negative), confirmed by antigen receptor gene analysis, which showed germ line configuration. Single-copy, clonally integrated FIV provirus was detected in tumor genomic DNA. FIV p24 antigen was not detected in tumor cells by immunostaining. This study provides the first evidence that the feline lentivirus may play a direct role in cell transformation under certain circumstances. 相似文献
98.
Mothers' and preschoolers' emotion talk was examined for age- (2, 3, and 4 years) and gender-related patterns in the use of labels and explanations. Although labels directly refer to emotions, explanations link emotion words to causal information. Children used emotion words mainly in labels. Boys' emotion talk showed an age increase: in contrast, the youngest group of girls talked about emotion much more frequently than did same-age boys, and this high frequency remained relatively stable across age. Mothers used more explanations than labels in emotion talk to boys but used similar amounts with girls. Further, their use of labels and explanations related to individual differences in the extent to which children talked about emotion. These findings are discussed in terms of language socialization?in particular, processes related to the socialization of emotion language and gender. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
99.
Maghasi AT Conklin SD Shtoyko T Piruska A Richardson JN Seliskar CJ Heineman WR 《Analytical chemistry》2004,76(5):1458-1465
Detection of lead and mercury by attenuated total internal reflectance spectroscopy coupled to stripping voltammetry is demonstrated. Changes in attenuation of light passing through an indium tin oxide optically transparent electrode (ITO-OTE) accompany the electrodeposition and stripping of lead and mercury on the electrode surface. The change in absorbance during stripping of electrodeposited metal constitutes the analytical response that enables detection over a range of 2.5 x 10(-7)-5 x 10(-5) and 5 x 10(-8)-5 x 10(-5) M for mercury and lead, respectively. The spectroelectrochemical responses of mercury and lead on the ITO surface are characterized and optimized with respect to solution conditions, the potential excitation signals used for deposition and stripping, and wavelength for detection. The deposited metals were examined by environmental scanning electron miscroscopy, and the electrodeposition pattern of lead and mercury was found to influence the optical response. 相似文献
100.
Outbound authentication for programmable secure coprocessors 总被引:1,自引:0,他引:1
A programmable secure coprocessor platform can help solve many security problems in distributed computing, particularly if coprocessor applications can participate as full-fledged parties in distributed cryptographic protocols. Thus, a generic platform must not only provide programmability, maintenance, and configuration in the hostile field, it must also provide outbound authentication for the entities that result. This paper offers our experiences in solving this problem for a high-end secure coprocessor product. This work required synthesis of a number of techniques, so that parties with different and dynamic views of trust can draw sound and complete conclusions about remote coprocessor applications. 相似文献