Influence of vehicle on historical pigments colour

The colour of a paint layer is determined by the pigments mixed with a vehicle and painted on a support. According to their chemical nature, the vehicles are substances that, after drying or hardening, produce an uniform and homogeneous layer. With the aim of quantifying the influence of the medium on the specification of the painting colour, we realized samples of paintings. We considered pigments and dyes of the artist's hues using the vehicles typical of ancient times: oils (especially linseed and poppy oils), casein and egg yolk, mixed according to old recipes. Measurements were performed by reflectance spectrophotometry observing the Spectral Reflectance Factor (%SRF) trend and calculating colour changes. The colorimetric study was carried out in the CIELAB colour space related to the CIE 1964 standard observer and with the D65 illuminant. The results were evaluated also on the basis of the distribution of pigment particles on paint layers observed by scanning electron microscopy.     

CMOS Transconductors With Nearly Constant Input Ranges Over Wide Tuning Intervals

Three different bias strategies aimed to reduce the effect of tuning on either the differential input range or the common-mode range of triode-region CMOS transconductors are presented. The method is applied to an original transconductor topology that is optimized to produce ultralow G_m values. A prototype circuit, which was designed with the 0.35-mum bipolar-CMOS-DMOS (BCD6) process of STMicroelectronics, is presented. The effectiveness and limitations of the method are characterized by means of electrical simulations     

UbiCrawler: a scalable fully distributed Web crawler

We report our experience in implementing UbiCrawler, a scalable distributed Web crawler, using the Java programming language. The main features of UbiCrawler are platform independence, linear scalability, graceful degradation in the presence of faults, a very effective assignment function (based on consistent hashing) for partitioning the domain to crawl, and more in general the complete decentralization of every task. The necessity of handling very large sets of data has highlighted some limitations of the Java APIs, which prompted the authors to partially reimplement them. Copyright © 2004 John Wiley & Sons, Ltd.     

PEG-templated mesoporous silica nanoparticles exclusively target cancer cells

Mesoporous silica nanoparticles (MSNs) have been proposed as DNA and drug delivery carriers, as well as efficient tools for fluorescent cell tracking. The major limitation is that MSNs enter cells regardless of a target-specific functionalization. Here we show that non functionalized MSNs, synthesized using a PEG surfactant-based interfacial synthesis procedure, do not enter cells, while a highly specific, receptor mediated, cellular internalization of folic acid (FOL) grafted MSNs (MSN-FOL), occurs exclusively in folate receptor (FR) expressing cells. Neither the classical clathrin pathway nor macropinocytosis is involved in the MSN endocytic process, while fluorescent MSNs (MSN-FITC) enter cells through aspecific, caveolae-mediated, endocytosis. Moreover, internalized particles seem to be mostly exocytosed from cells within 96 h. Finally, cisplatin (Cp) loaded MSN-FOL were tested on cancerous FR-positive (HeLa) or normal FR-negative (HEK293) cells. A strong growth arrest was observed only in HeLa cells treated with MSN-FOL-Cp. The results presented here show that our mesoporous nanoparticles do not enter cells unless opportunely functionalized, suggesting that they could represent a promising vehicle for drug targeting applications.     

Effect of in vitro fertilization on gene expression and development of mouse preimplantation embryos

In vitro culture (IVC) of preimplantation mouse embryos is associated with changes in gene expression. It is however, not known if the method of fertilization affects the global pattern of gene expression. We compared gene expression and development of mouse blastocysts produced by in vitro fertilization (IVF) versus blastocysts fertilized in vivo and cultured in vitro from the zygote stage (IVC) versus control blastocysts flushed out of the uterus on post coital day 3.5. The global pattern of gene expression was assessed using the Affymetrix 430 2.0 chip. It appears that each method of fertilization has a unique pattern of gene expression and development. Embryos cultured in vitro had a reduction in the number of trophoblastic cells (IVF 33.5 cells, IVC 39.9 cells, and 49.6 cells in the in vivo group) and, to a lesser degree, of inner cell mass cells (12.8, 11.7, and 13.8 respectively). The inner cell mass nuclei were larger after culture in vitro (140 microm(2), 113 microm(2), and 86 microm(2) respectively). Although a high number of genes (1912) was statistically different in the IVF cohort when compared with the in vivo control embryos, the magnitude of the changes in gene expression were low and only a minority of genes (29 genes) was changed more than fourfold. Surprisingly, IVF embryos were different from IVC embryos (3058 genes were statistically different, but only three changed more than fourfold). Proliferation, apoptosis, and morphogenetic pathways are the most common pathways altered after IVC. Overall, IVF and embryo culture have a profound effect on gene expression pattern and phenotype of mouse preimplantation embryos.     

Hydrogenation of carbon monoxide under mechanical activation conditions

The work focuses on the hydrogenation of carbon monoxide over solid catalysts undergoing mechanical activation by ball milling. The rate of carbon monoxide conversion at individual ball impacts was estimated by measuring the impact frequency, the mass of powders involved in individual impacts and their duration. The rate of the mechanochemical hydrogenation process was compared with the one of the corresponding thermal process. An enhancement of the catalyst activity under mechanical activation conditions is observed.     

SAR amplitude probability density function estimation based on a generalized Gaussian model.

In the context of remotely sensed data analysis, an important problem is the development of accurate models for the statistics of the pixel intensities. Focusing on synthetic aperture radar (SAR) data, this modeling process turns out to be a crucial task, for instance, for classification or for denoising purposes. In this paper, an innovative parametric estimation methodology for SAR amplitude data is proposed that adopts a generalized Gaussian (GG) model for the complex SAR backscattered signal. A closed-form expression for the corresponding amplitude probability density function (PDF) is derived and a specific parameter estimation algorithm is developed in order to deal with the proposed model. Specifically, the recently proposed "method-of-log-cumulants" (MoLC) is applied, which stems from the adoption of the Mellin transform (instead of the usual Fourier transform) in the computation of characteristic functions and from the corresponding generalization of the concepts of moment and cumulant. For the developed GG-based amplitude model, the resulting MoLC estimates turn out to be numerically feasible and are also analytically proved to be consistent. The proposed parametric approach was validated by using several real ERS-1, XSAR, E-SAR, and NASA/JPL airborne SAR images, and the experimental results prove that the method models the amplitude PDF better than several previously proposed parametric models for backscattering phenomena.     

Examination of the Novel Sigma-1 Receptor Antagonist,SI 1/28, for Antinociceptive and Anti-allodynic Efficacy against Multiple Types of Nociception with Fewer Liabilities of Use

Neuropathic pain is a significant problem with few effective treatments lacking adverse effects. The sigma-1 receptor (S1R) is a potential therapeutic target for neuropathic pain, as antagonists for this receptor effectively ameliorate pain in both preclinical and clinical studies. The current research examines the antinociceptive and anti-allodynic efficacy of SI 1/28, a recently reported benzylpiperazine derivative and analog of the S1R antagonist SI 1/13, that was 423-fold more selective for S1R over the sigma-2 receptor (S2R). In addition, possible liabilities of respiration, sedation, and drug reinforcement caused by SI 1/28 have been evaluated. Inflammatory and chemical nociception, chronic nerve constriction injury (CCI) induced mechanical allodynia, and adverse effects of sedation in a rotarod assay, conditioned place preference (CPP), and changes in breath rate and locomotor activity were assessed after i.p. administration of SI 1/28. Pretreatment with SI 1/28 produced dose-dependent antinociception in the formalin test, with an ED₅₀ (and 95% C.I.) value of 13.2 (7.42–28.3) mg/kg, i.p. Likewise, SI 1/28 produced dose-dependent antinociception against visceral nociception and anti-allodynia against CCI-induced neuropathic pain. SI 1/28 demonstrated no impairment of locomotor activity, conditioned place preference, or respiratory depression. In summary, SI 1/28 proved efficacious in the treatment of acute inflammatory pain and chronic neuropathy without liabilities at therapeutic doses, supporting the development of S1R antagonists as therapeutics for chronic pain.     

Mitochondrial a Kinase Anchor Proteins in Cardiovascular Health and Disease: A Review Article on Behalf of the Working Group on Cellular and Molecular Biology of the Heart of the Italian Society of Cardiology

Second messenger cyclic adenosine monophosphate (cAMP) has been found to regulate multiple mitochondrial functions, including respiration, dynamics, reactive oxygen species production, cell survival and death through the activation of cAMP-dependent protein kinase A (PKA) and other effectors. Several members of the large family of A kinase anchor proteins (AKAPs) have been previously shown to locally amplify cAMP/PKA signaling to mitochondria, promoting the assembly of signalosomes, regulating multiple cardiac functions under both physiological and pathological conditions. In this review, we will discuss roles and regulation of major mitochondria-targeted AKAPs, along with opportunities and challenges to modulate their functions for translational purposes in the cardiovascular system.