Introduction and expression of the cry1Ac gene of Bacillus thuringiensis in a cereal-associated bacterium, Bacillus polymyxa

The abilities of Bacillus polymyxa and Bacillus thuringiensis to survive on the rice phyllospere were compared; it was found that B. polymyxa colonizes the crop better. This study also showed that B. polymyxa inoculation to rice plants increased the shoot and the root growth of the crop. Efforts were made to introduce the cry1Ac gene of B. thuringiensis subsp. kurstaki into B. polymyxa so that the application of such transgenic B. polymyxa strains would prove to be dually beneficial to rice crops both as a biopesticide and as a biofertilizer. Immunoblot analysis of the recombinant organism containing the cry1Ac gene, strain BP113, indicated efficient expression of this gene in the heterologous host. Bioassays with the first instar larvae of the yellow stem borer of rice (Scirpophaga incertulas) revealed that the protein preparations from BP113 were toxic.     

HIV infection amongst leprosy patients in south India

We use the trapezoidal lamellar incision as standard construction for sutureless self-sealing wound closure in our clinic: a 7 mm incision for phacoemulsification and implantation of a 6.5 mm optic diameter PMMA posterior chamber less (PCL) and an incision up to 11 mm for ECCE with standard PCL. The operation can be performed in a nearly closed system because of the self-sealing wound construction. Experiments in cadaver eyes showed that the wound closure of a 7 mm incision with the no-stitch technique ruptured at a pressure five times greater than the cross-stitch-sutured corneoscleral incision and in an 11 mm incision four times greater (602 +/- 149 mmHg and 150 +/- 29 mmHg). Due to the high stability of the wound closure, no sutures are necessary. Complications arising in connection with sutures are therefore avoided. Clinically, two typical postoperative complications were observed: hypotony occurred in 1% and anterior chamber hemorrhage in 5%. These complications are exclusively caused by imperfection of the internal corneal opening.     

Heuristics for operator allocation and sequencing in just-in-time flow line manufacturing cell

Group technology tries to exploit the similarity between parts and machines and forms machine groups and part families. Just-in-time production tries to manufacture the parts whenever required there by reducing the inventory and eliminating waste.

In order to apply JIT in a GT cell, the cell is divided into modules and parts move from one module to another in small transfer batches. This paper addresses the problem of operator allocation for the modules and sequencing the variety of parts with the objective of minimizing the makespan.

Six different methodologies have been presented and the results compared in terms of makespan and computational time.     

Effects of co-adsorbate and additive on the performance of dye-sensitized solar cells: A photophysical study

The effects of deoxycholic acid (DCA) in a dye solution as a co-adsorbate and guanidinium thiocyanate (GuSCN) in an electrolyte as an additive, on the photovoltaic performance of dye-sensitized solar cells (DSSCs) based on organic dye containing thiophene and fluorine segments (FL dye1) and black dye (BD) were investigated. The presence of DCA, up to 2 mM, increases both the photovoltage and photocurrent of the DSSC incorporating BD and further addition leads to decrease in the photocurrent. On the other hand, in the case of the DSSC containing FL dye1, the photocurrent decrease and photovoltage increase with the increase of DCA concentration. The pulsed laser measurement showed that electron lifetime (τ_e) of BD is higher than that of FL dye1 on a TiO₂ electrode and the electron diffusion coefficient (D_e) of the latter is higher than that of the former where there is little deviation for both τ_e and D_e with the addition of the DCA. The addition of GuSCN into electrolytes does enhance the photovoltage for DSSCs containing either FL dye1 or BD. This can be explained by the further adsorption of guanidinium cations following the dye adsorption that facilitates the self-assembly of dye molecules so as to either reduce the dark current or positively shift the conduction band edge of the TiO₂. The value of τ_e increases and the value of D_e decreases as a result of GuSCN addition.     

On the use of triethylamine hydroiodide as a supporting electrolyte in dye-sensitized solar cells

For the first time, the application of a molten salt, triethylamine hydroiodide (THI), as a supporting electrolyte was investigated for the dye-sensitized solar cells (DSSCs). Titanium dioxide (TiO₂) electrode was modified by incorporation of high- and low-molecular weight poly(ethylene glycol) along with TiO₂ nanoparticles of two different sizes (300 nm (30 wt%) and 20 nm (70 wt%)). The highest apparent diffusion coefficient (D) of 8.12×10⁻⁶ cm² s⁻¹ was obtained for I⁻ (0.5 M of THI) from linear sweep voltammetry (LSV). Short-circuit current density (J_sc) increases with the concentration of THI whereas open-circuit potential (V_oc) remains the same. Optimum J_sc (19.28 mA cm⁻²) and V_oc (0.7 V) with a highest conversion efficiency (η) of 8.45% were obtained for the DSSC containing 0.5 M of THI/0.05 M I₂/0.5 M TBP in CH₃CN. It is also observed that the J_sc and η of the DSSC mainly relates with the D values of I⁻ and charge-transfer resistances such as R_ct1 and R_ct2 operating along Pt/TiO₂ electrolyte interface, obtained from LSV and electrochemical impedance spectroscopy (EIS). For comparison, tetraethylammonium iodide (TEAI) and LiI were also selected as supporting electrolytes. Though both the THI and TEAI have similar structures, replacement of one methyl group by hydrogen improves the efficiency of the DSSC containing the former electrolyte. Further, the DSSC containing THI exhibits higher J_sc and η than LiI (7.70%), from which it is concluded that THI may be used as an efficient and alternative candidate to replace LiI in the current research of DSSCs.     

Solution NMR of polypeptides hyperpolarized by dynamic nuclear polarization

Hyperpolarization of nuclear spins through techniques such as dynamic nuclear polarization (DNP) can greatly increase the signal-to-noise ratio in NMR measurements, thus eliminating the need for signal averaging. This enables the study of many dynamic processes which would otherwise not be amenable to study by NMR spectroscopy. A report of solid- to liquid-state DNP of a short peptide, bacitracin A, as well as of a full-length protein, L23, is presented here. The polypeptides are hyperpolarized at low temperature and dissolved for NMR signal acquisition in the liquid state in mixtures of organic solvent and water. Signal enhancements of 300-2000 are obtained in partially deuterated polypeptide when hyperpolarized on (13)C and of 30-180 when hyperpolarized on (1)H. A simulated spectrum is used to identify different resonances in the hyperpolarized (13)C spectra, and the relation between observed signal enhancement for various groups in the protein and relaxation parameters measured from the hyperpolarized samples is discussed. Thus far, solid- to liquid-state DNP has been used in conjunction with small molecules. The results presented here, however, demonstrate the feasibility of hyperpolarizing larger proteins, with potential applications toward the study of protein folding or macromolecular interactions.     

Synthesis of tungsten oxide (W18O49) nanosheets utilizing EDTA salt by microwave irradiation method

We report the synthesis of crystalline W₁₈O₄₉ with nanosheet like morphology by low cost microwave irradiation method without employing hydrothermal process for the first time. Initially, WO₃·H₂O was synthesized using ethylenediaminetetraacetic acid (EDTA) as surface modulator. The product was annealed at 600 °C for 6 h in ambient atmosphere in order to obtain anhydrous tungsten oxide W₁₈O₄₉. Powder X-ray diffraction results confirmed the as prepared WO₃·H₂O to be orthorhombic and W₁₈O₄₉ to be monoclinic phase, respectively. Transmission electron micrographs (TEM) revealed that the W₁₈O₄₉ nanosheets have the average dimensions of the order of 250 nm in length and around 150 nm in width. UV-visible diffusion reflectance spectroscopic (DRS) studies revealed the band gap energies to be 3.28 and 3.47 eV for WO₃·H₂O and W₁₈O₄₉ samples, respectively. The growth mechanism of two dimensional W₁₈O₄₉ nanosheets is discussed.     

Synthesis of nickel hydroxide/reduced graphene oxide composite thin films for water splitting application

Facile synthesis of highly efficient and low-cost electrocatalyst for oxygen evolution reaction (OER) is important for large-scale hydrogen production. Herein, nickel hydroxide/reduced graphene oxide (Ni(OH)₂/rGO) composite thin film was fabricated using dip-coating followed by electrodeposition method on Ni foam substrate at room temperature. The deposited composite film shows amorphous nature with ultra-thin Ni(OH)₂ nanosheets vertically coated on rGO surface, which provides large electrochemical surface area and abundant catalytically active sites. It exhibits a low overpotential of 260 mV @10 mA cm⁻² as compared to the pristine electrodes and excellent long-term stability up to 20 hours in 1 M KOH solution. The electrochemical active surface area and Tafel slope of the composite electrode are 20.2 mF cm⁻² and 35 mV dec⁻¹, respectively. The superior water oxidation performance is a result of high catalytically active sites and improved conductivity of the composite electrode.     

Progress of MWCNT,Al2O3, and CuO with water in enhancing the photovoltaic thermal system

Hybrid photovoltaic thermal system is an effective method to convert solar energy into electrical and thermal energy. However, its effectiveness is widely affected due to the high temperature of photovoltaic panel, and it can be minimized by employing nanofluids to the PV/T systems. In this research, the effect of various nanoparticles on the PV/T systems was studied experimentally. The nanofluids Al₂O₃, CuO, and multiwall carbon nanotube (MWCNT) were dispersed with water at different volume fractions of 0, 0.5, 1, 2.5, and 5 (vol%) using ultrasonication process. The effect of nanomaterials on viscosity and density was classified. All tests were carried out in an outdoor laboratory setup for calibrating the PV temperatures, thermal conductivity, electrical power, electrical efficiency, and overall efficiency. In addition, the energy analyses were also made to estimate the loss of heat owing to the nanofluids. Results show that use of the nanofluid increased the electric power and electrical efficiency of PV/T compared with water. Furthermore, MWCNT and CuO reduced the cell temperature by 19%. Consequently, the nanofluids MWCNT, Al₂O₃, and CuO produced the impressive values of 60%, 55%, and 52% increase in an average electrical efficiency than conventional PV. Particularly, the MWCNT produced superior results compared with other materials. It is evidently clear from the result that the introduction of the nanofluid increases the thermal efficiency without adding any extra energy to the system. Moreover, insertion of Al₂O₃, CuO, and MWCNT on PV/T system increases the exergy efficiency more than conventional PV module.     

Effect of rotation of feed and seed rods on the quality of Na0.75CoO2 single crystal grown by traveling solvent floating zone method

High purity Na_0.75CoO₂ single crystals have been grown by floating zone method. We found the rotation of feed and seed rods play a crucial role in growing high quality single crystal. Systematic investigations suggest the occurrence of a phase separation at microscopic level, such as the separation into Na-rich and Na-poor domains during the growth, and formation of impurity phase(s) depending on growth conditions. Na_xCoO₂ (x = 0.30, 0.60) crystals have been prepared by sodium deintercalation from Na_0.75CoO₂. Powder X-ray and energy dispersive X-ray analyses have confirmed the phase purity and homogeneity of the samples. Magnetic susceptibility measurements of x = 0.60 and 0.75 crystals indicate a bulk phase transition at 22 K and an anomaly around 339 K and 334 K respectively which are attributed to structural transition. In addition some unique features related to Na_0.60CoO₂ were observed.