Possible Role of GnIH as a Novel Link between Hyperphagia-Induced Obesity-Related Metabolic Derangements and Hypogonadism in Male Mice

Gonadotropin-inhibitory hormone (GnIH) is a reproductive inhibitor and an endogenous orexigenic neuropeptide that may be involved in energy homeostasis and reproduction. However, whether GnIH is a molecular signal link of metabolism and the reproductive system, and thus, regulates reproductive activity as a function of the energy state, is still unknown. In the present study, we investigated the involvement of GnIH in glycolipid metabolism and reproduction in vivo, and in the coupling between these two processes in the testis level. Our results showed that chronic intraperitoneal injection of GnIH into male mice not only increased food intake and altered meal microstructure but also significantly elevated body mass due to the increased mass of liver and epididymal white adipose tissue (eWAT), despite the loss of testicular weight. Furthermore, chronic intraperitoneal administration of GnIH to male mice resulted in obesity-related glycolipid metabolic derangements, showing hyperlipidemia, hyperglycemia, glucose intolerance, and insulin resistance through changes in the expression of glucose and lipid metabolism-related genes in the pancreas and eWAT, respectively. Interestingly, the expression of GnIH and GPR147 was markedly increased in the testis of mice under conditions of energy imbalance, such as fasting, acute hypoglycemia, and hyperglycemia. In addition, chronic GnIH injection markedly inhibited glucose and lipid metabolism of mice testis while significantly decreasing testosterone synthesis and sperm quality, inducing hypogonadism. These observations indicated that orexigenic GnIH triggers hyperphagia-induced obesity-related metabolic derangements and hypogonadism in male mice, suggesting that GnIH is an emerging candidate for coupling metabolism and fertility by involvement in obesity and metabolic disorder-induced reproductive dysfunction of the testes.     

UASB+SBR工艺处理糖果废水

针对糖果废水的特点,采用UASB SBR工艺处理箭牌糖果(中国)有限公司番禺工厂的糖果废水,通过介绍处理工艺流程和工艺调试运行过程,提出了影响工艺调试的因素及相应的控制措施。工程实践证明,UASB SBR工艺是糖果废水有效的处理工艺,出水水质达到《水污染物排放限值》(DB44/26-2001)中第二时段的三级标准。     

Genome-Wide Characterization and Phylogenetic Analysis of GSK Genes in Maize and Elucidation of Their General Role in Interaction with BZR1

Glycogen synthase kinase-3 (GSK-3) is a nonreceptor serine/threonine protein kinase that is involved in diverse processes, including cell development, photomorphogenesis, biotic and abiotic stress responses, and hormone signaling. In contrast with the deeply researched GSK family in Arabidopsis and rice, maize GSKs’ common bioinformatic features and protein functions are poorly understood. In this study, we identified 11 GSK genes in the maize (Zea mays L.) genome via homologous alignment, which we named Zeama;GSKs (ZmGSKs). The results of ZmGSK protein sequences, conserved motifs, and gene structures showed high similarities with each other. The phylogenetic analyses showed that a total of 11 genes from maize were divided into four clades. Furthermore, semi-quantitative RT-PCR analysis of the GSKs genes showed that ZmGSK1, ZmGSK2, ZmGSK4, ZmGSK5, ZmGSK8, ZmGSK9, ZmGSK10, and ZmGSK11 were expressed in all tissues; ZmGSK3, ZmGSK6, and ZmGSK7 were expressed in a specific organization. In addition, GSK expression profiles under hormone treatments demonstrated that the ZmGSK genes were induced under BR conditions, except for ZmGSK2 and ZmGSK5. ZmGSK genes were regulated under ABA conditions, except for ZmGSK1 and ZmGSK8. Finally, using the yeast two-hybrid and BiFC assay, we determined that clads II (ZmGSK1, ZmGSK4, ZmGSK7, ZmGSK8, and ZmGSK11) could interact with ZmBZR1. The results suggest that clade II of ZmGSKs is important for BR signaling and that ZmGSK1 may play a dominant role in BR signaling as the counterpart to BIN2. This study provides a foundation for the further study of GSK3 functions and could be helpful in devising strategies for improving maize.     

多功能油层保护剂JQD的研制与应用

针对新疆小拐油田裂缝-孔隙型储层特点,开发研制了一种新型的兼具防漏堵漏及油层保护功能的JQD处理剂。该处理剂具有较好的油溶率、酸溶率,与现有钻井完井液体系配伍性良好,加工粒径可调,对1—4mm的裂缝堵漏效果良好。动态损害实验表明,JQD体系的岩心渗透率恢复值不小于80％。现场试验三口表明,JQD与钻井液配伍性良好,防漏堵漏效果显著,性能调整及维护方便,起下钻及电测顺利。三口井投产后,两口井产量分别为30和28t/d,一口井产气量为1.1×10~4m~3/d,说明储层得到了有效的保护,实现了“堵得住、解得开”的目标。     

超细银粉制备技术的现状与展望

本文介绍了超细银粉制备技术的各种方法,尤其重点介绍了真空油面蒸发法和沉淀过滤法等。并对各种方法的优缺点作了比较。同时展望了今后超细银粉制备技术的发展方向。     

降低单层混合建筑整体平移造价的技术措施

针对某单层混合结构建筑整体平移工程,提出在平移过程中保证其强度和刚度的同时又降低工程造价的具体措施,该措施与传统的房屋整体平移方法相比,简单、实用、工期短、强度和刚度易于控制、造价低。可为类似建筑整体平移工程提供参考。     

Strengthening and toughening mechanism of a Cu-bearing high-strength low-alloy steel with refined tempered martensite/bainite (M/B) matrix and minor inter-critical ferrite

The microstructure–mechanical property relationship of a Cu-bearing low-carbon high-strength low-alloy steel, subjected to a novel multistage heat treatment inc...     

Insights into the Reaction Network and Mechanism of Green Aerobic Oxidative Esterification of Methacrolein over Different Heterogeneous Catalysts

The oxidative esterification of methacrolein (MAL) is an important way to prepare high-valued methyl methacrylate (MMA), but this process is ultra-complex due t...     

Essential oil composition and antimicrobial activity of Sphallerocarpus gracilis seeds against selected food-related bacteria

Sphallerocarpus gracilis from China is a little-investigated edible and medicinal plant. In the present study, the essential oil composition from S. gracilis seeds was investigated by GC and GC–MS. A total of 34 compounds representing 94.69% of the essential oil were tentatively identified. The main constituents were p-cymene (17.42%), γ-terpinene (25.58%) and α-asarone (33.12%). The antimicrobial activity, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) of the essential oil from S. gracilis seeds were evaluated against eight Gram-positive bacteria (Listeria monocytogenes, Staphylococcus aureus, Staphylococcus epidermidis, Micrococcus luteus, Bacillus megaterium, Bacillus cereus, Bacillus coagulans and Bacillus subtilis), four Gram-negative bacteria (Salmonella enteritidis, Salmonella typhimurium, Klebsiella pneumoniae and Escherichia coli) and one fungus (Aspergillus niger). Results revealed that the essential oil from S. gracilis seeds exhibited significant in vitro antimicrobial property. Among all the tested microorganisms, the essential oil showed the strongest inhibitory effect against K. pneumoniae, whereas no inhibitory effect was found against L. monocytogenes and A. niger. Additionally, scanning electron microscopy (SEM) was used to observe morphological changes of bacteria treated with the essential oil from S. gracilis seeds. SEM observations confirmed the physical damage and considerable morphological alteration to the tested microorganisms treated with the essential oil. The data of this study suggests that the essential oil from S. gracilis seeds has great potential for application as a natural antimicrobial agent to preserve food.     

中国联通采购管理系统应用分析

介绍中国联通采购管理系统建设面临的业务现状、问题、系统的解决方案,并对系统的应用价值进行了分析,提出未来发展的展望。