Cytotoxicity of advanced glycation endproducts is mediated by oxidative stress

Non-enzymatic glycation of proteins with reducing sugars and subsequent transition metal catalysed oxidations leads to the formation of protein bound "advanced glycation endproducts" (AGEs). They accumulate on long-lived proteins and are for example structural components of the beta-amyloid plaques in Alzheimer's disease. Since the oxidation of glycated proteins as well as the interaction of AGEs with cell surface receptors produces superoxide radicals, it was tested in BHK 21 hamster fibroblast cells and SH-SY5Y human neuroblastoma cells if AGEs can exert cytotoxic effects on cells. Cell viability was assessed with three independent tests: MTT-assay (activity of the mitochondrial respiratory chain), lactate dehydrogenase assay (release of cytoplasmatic enzymes, membrane integrity) and Neutral Red assay (active uptake of a hydrophilic dye). Two model AGEs, chicken egg albumin-AGE and BSA-AGE, both caused significant cell death in a dose-dependent manner. The cytotoxic effects of AGEs could be attenuated by alpha-ketoglutarate and pyruvate, by antioxidants such as thioctic acid and N-acetylcysteine, and by aminoguanidine, an inhibitor of nitric oxide synthase. This suggests that reactive oxygen species as well as reactive nitrogen species contribute to AGE mediated cytotoxicity. Since AGEs accumulate on beta-amyloid plaques in AD over time, they may additionally contribute to oxidative stress, cell damage, functional loss and even neuronal cell death in the Alzheimer's disease brain.     

Normalized Interactions between autonomous agents

The CEC Project GOAL (Esprit 6283) aims to develop generic software tools to support a new project management paradigm, in which projects are collaborative, decentralised and inter-organizational. To support inter-organizational interaction, communication and cooperation, we are developing a design framework for formalizing the flow of information between organizations, specifying access to and provision of project services, and defining project-wide standards and procedures. This framework is based on normalizing interactions between autonomous software agents by specifying messages and protocols for inter-agent communication and cooperation. This paper reviews the framework, and then focusses on the specification and implementation of a case study, the automation of a distributed document review procedure. This is both a successful proof of concept and a demonstration of how Artificial Intelligence technologies can support inter-organizational project management. It also points the way to agent brokering, an enhancement of object brokering in distributed open systems, where the satisfaction of service requests can be subject to negotiation.Supported by CEC Esprit Project GOAL (Esprit 6283) and CEC Esprit BRA Medlar II (Esprit 6471).Supported by CEC Esprit Project GOAL (Esprit 6283).     

Partitioning large networks without breaking communities

The identification of cohesive communities is a key process in social network analysis. However, the algorithms that are effective for finding communities do not scale well to very large problems, as their time complexity is worse than linear in the number of edges in the graph. This is an important issue for those interested in applying social network analysis techniques to very large networks, such as networks of mobile phone subscribers. In this respect, the contributions of this paper are twofold. First, we demonstrate these scaling issues using a prominent community-finding algorithm as a case study. Then, we show that a two-stage process, whereby the network is first decomposed into manageable subnetworks using a multilevel graph partitioning procedure, is effective in finding communities in networks with more than 10⁶ nodes.     

An Achilles heel: denial of service attacks on Australian critical information infrastructures

Critical, or national, information infrastructure protection, referred to as either CIIP or NIIP, has been highlighted as a critical factor in overall national security by the United States, the United Kingdom, India and the European Community. As nations move inexorably towards so-called ‘digital economies’, critical infrastructure depends on information systems to process, transfer, store and exchange information through the Internet. Electronic attacks such as denial of service attacks on critical information infrastructures challenge the law and raise concerns. A myriad of issues potentially plague the protection of critical information infrastructures owing to the lack of legal regulation aimed at ensuring the protection of critical information infrastructures. This paper will highlight the legal concerns that relate to the denial of service attacks on critical information infrastructures and provide an introductory overview of the law as it relates to CIIP in Australia.     

3DVIA Composer进阶操作实战

设计研发最繁琐的工作,莫过于设计进行中及完成后所需递交的技术文件报告.为了解决这个棘手的问题,SolidWorks提供了3DVIA Composer文件发布系统,结合绘图者最想改善的速度问题,并加上支持3D同步变更的优势,大幅度降低了复杂3D的设计更新.可以整合图片、Office文件、PDF和HTML网页格式的各种规格,产生的编排布局规格变化,客户可由开放客制化的工具自行编排.     

An observational comparison of the older and younger bus passenger experience in a developing world city

This study was an unobtrusive observational analysis of 333 older and younger bus passengers in Guadalajara, Mexico. A set of data were collected for each observed passenger, as well as more general observations related to driver behaviour, bus design and bus service characteristics. There were significant differences between older and younger passengers in terms of boarding and alighting times, use of handrails, seat location preferences, passenger stability and coping strategies in order to maintain postural stability. The conditions of travel are conducive to a poor passenger experience for the older passengers in particular. Although the problems may be attributed to bus design and driver behaviour typical of that in developing countries, they are also influenced by the wider transport infrastructure, and a lack of a regulatory regime which places drivers under time pressure and in direct competition with each other.

Practitioner Summary: Bus services must cater for all ages of passengers, including the elderly. This unobtrusive observational study investigated the passenger experience in a developing world city. Bus and wider service design were found to compromise the journey experience, with the older users being particularly negatively impacted. Design recommendations are provided.     

Big data in the public sector: Uncertainties and readiness

Big data is being implemented with success in the private sector and science. Yet the public sector seems to be falling behind, despite the potential value of big data for government. Government organizations do recognize the opportunities of big data but seem uncertain about whether they are ready for the introduction of big data, and if they are adequately equipped to use big data. This paper addresses those uncertainties. It presents an assessment framework for evaluating public organizations’ big data readiness. Doing so demystifies the concept of big data, as it is expressed in terms of specific and measureable organizational characteristics. The framework was tested by applying it to organizations in the Dutch public sector. The results suggest that organizations may be technically capable of using big data, but they will not significantly gain from these activities if the applications do not fit their organizations and main statutory tasks. The framework proved helpful in pointing out areas where public sector organizations could improve, providing guidance on how government can become more big data ready in the future.     

Scientific sketching for collaborative VR visualization design

We present four studies investigating tools and methodologies for artist-scientist-technologist collaboration in designing multivariate, virtual reality (VR) visualizations. Design study 1 identifies the promise of 3D drawing-style interfaces for VR design and also establishes limitations of these tools with respect to precision and support for animation. Design study 2 explores animating artist-created visualization designs with scientific 3D fluid flow data. While results captured an accurate sense of flow that was advantageous as compared to the results of study 1, the potential for visual exploration using the design tools tested was limited. Design study 3 reveals the importance of a new 3D interface that overcomes the precision limitation found in study 1 while remaining accessible to artist collaborators. Drawing upon previous results, design study 4 engages collaborative teams in a design process that begins with traditional paper sketching and moves to animated, interactive, VR prototypes "sketched" by designers in VR using interactive 3D tools. Conclusions from these four studies identify important characteristics of effective artist-accessible VR visualization design tools and lead to a proposed formalized methodology for successful collaborative design that we expect to be useful in guiding future collaborations. We call this proposed methodology Scientific Sketching.