Tag-free microfluidic separation of cells against multiple markers

Conventional cell separation against multiple markers generally requires the attachment of antibody tags, typically fluorescent or magnetic, to selected cell types in a heterogeneous suspension. This work describes how such separation can be accomplished in a series of microfluidic systems without the need for such tags. Two capture stages containing antibody-functionalized alginate hydrogels are utilized for the isolation of CD34+ and Flk1+ cells from untreated, whole human blood. The capture-release capability of these degradable coatings is harnessed by a mixing chamber and a simple valving system such that the suspension emerging from the first capture stage is prepared for the second capture stage for further enrichment. With this configuration, we demonstrate the isolation of CD34+/Flk1+ endothelial progenitor cells from blood enabled by the depletion of CD34+/Flk1-hematopoietic stem cells population. This ability to achieve isolation of cells against multiple markers in an untagged separation method is of particular significance in applications involving cell implantation-based therapeutics including tissue engineering and molecular analysis.     

A modified approach for collimation testing using Lau interferometry

A modified approach for the collimation testing of an incoherent optical beam using the Lau effect coupled with moiré readout is proposed. In the experimental set-up a white-light incoherent source illuminates a coarse grating G_1. The collimating lens is mounted on a translation stage, so that the grating G₁ can be adjusted to be at the ‘in-focus’, ‘at-focus’ or ‘out-of-focus’ position. Grating G₂ and grating G₃ are mounted on a precision rotational stage and are so positioned that the lines of the gratings make a small but equal and opposite angle with the vertical. The inclination angle of the resulting moiré fringe demonstrates the quality of collimation of optical beam. The proposed technique can also be conveniently used for measurement of the focal length of a collimating lens, at a very low cost.     

Significant physical attributes affecting quality of Indian black (CTC) tea

In this research work, an attempt was made to discriminate different grades of black crush-tear-curl (CTC) tea based upon their physical attributes such as color of brewed liquor, texture, size and shape of the tea granules obtained by machine vision technique. The principal component analysis (PCA) was applied over two types of data. First, tea samples with seven different quality grades but same mechanical grading and second, samples with same quality grade but nine different mechanical grades (Brokens, Fannings and Dust) were considered for analysis, respectively. The results of PCA showed that best discrimination (100%) in both types of data was given by color attributes only. Correlations among tea samples and physical attributes were determined. Based upon these results it may be concluded that color only attributes are the most significant and sufficient for quantification of tea quality whereas other physical attributes contribute so little to quality estimation that they may be ignored.     

Stacking dependent electronic structure and transport in bilayer graphene nanoribbons

The stacking-dependent electronic structure and transport properties of bilayer graphene nanoribbons suspended between gold electrodes are investigated using density functional theory coupled with non-equilibrium Green’s functional method. We find substantially enhanced electron transmission as well as tunneling currents in the AA stacking of bilayer nanoribbons compared to either single-layer or AB stacked bilayer nanoribbons. Interlayer separation between the nanoribbons appears to have a profound impact on the conducting features of the bilayer nanoribbons, which is found to be closely related to the topology and overlap between the edge-localized π orbitals.     

Efficiency enhancement in organic solar cells with ferroelectric polymers

The recombination of electrons and holes in semiconducting polymer-fullerene blends has been identified as a main cause of energy loss in organic photovoltaic devices. Generally, an external bias voltage is required to efficiently separate the electrons and holes and thus prevent their recombination. Here we show that a large, permanent, internal electric field can be ensured by incorporating a ferroelectric polymer layer into the device, which eliminates the need for an external bias. The electric field, of the order of 50 V μm(-1), potentially induced by the ferroelectric layer is tens of times larger than that achievable by the use of electrodes with different work functions. We show that ferroelectric polymer layers enhanced the efficiency of several types of organic photovoltaic device from 1-2% without layers to 4-5% with layers. These enhanced efficiencies are 10-20% higher than those achieved by other methods, such as morphology and electrode work-function optimization. The devices show the unique characteristics of ferroelectric photovoltaic devices with switchable diode polarity and tunable efficiency.     

Removal of mercury by foam fractionation using surfactin, a biosurfactant

The separation of mercury ions from artificially contaminated water by the foam fractionation process using a biosurfactant (surfactin) and chemical surfactants (SDS and Tween-80) was investigated in this study. Parameters such as surfactant and mercury concentration, pH, foam volume, and digestion time were varied and their effects on the efficiency of mercury removal were investigated. The recovery efficiency of mercury ions was highly sensitive to the concentration of the surfactant. The highest mercury ion recovery by surfactin was obtained using a surfactin concentration of 10 × CMC, while recovery using SDS required < 10 × CMC and Tween-80 >10 × CMC. However, the enrichment of mercury ions in the foam was superior with surfactin, the mercury enrichment value corresponding to the highest metal recovery (10.4%) by surfactin being 1.53. Dilute solutions (2-mg L(-1) Hg(2+)) resulted in better separation (36.4%), while concentrated solutions (100 mg L(-1)) enabled only a 2.3% recovery using surfactin. An increase in the digestion time of the metal solution with surfactin yielded better separation as compared with a freshly-prepared solution, and an increase in the airflow rate increased bubble production, resulting in higher metal recovery but low enrichment. Basic solutions yielded higher mercury separation as compared with acidic solutions due to the precipitation of surfactin under acidic conditions.     

Hydraulic System Component Storage with Military Hydraulic Fluids

In the military aerospace community, most hydraulic fluid pumps and components are currently being stored in rust inhibited fluids containing barium dinonylnaphthalene sulfonate (BSN). Fluids containing barium are hazardous waste after use, with expensive disposal, and have caused operational problems in aircraft hydraulic systems including helicopters and fighter aircraft. In this program, bearings and pistons were stored in jars containing both operational hydraulic fluids (MIL-PRF-83282, MIL-PRF-87257, and MIL-PRF-5606) and rust-inhibited hydraulic fluids containing BSN (MIL-PRF-46170 and MIL-PRF-6083). In addition, hydraulic pumps were filled with MIL-PRF-83282, MIL-PRF-87257, and MIL-PRF-46170. Hydraulic pumps were not filled with MIL-PRF-5606 or MIL-PRF-6083 because these hydraulic fluids are being phased out of military aerospace applications as operational and storage fluids, respectively. Jars, containing bearings and pistons, as well as hydraulic pumps, were stored for up to three years in a laboratory environment to determine if operational fluids would protect them from rusting during storage. After each year, the bearings, pistons, and pumps were inspected for corrosion. At the end of three years of storage, pumps were endurance tested using fresh operational fluid, MIL-PRF-83282. The bearings, pistons, and pumps showed no rusting for the duration of storage with either operational or storage fluids. The pumps stored with the operational fluids, MIL-PRF-83282 and MIL-PRF-87257, were in better condition than the pump stored with the rust-inhibited fluid. The operational hydraulic fluids, MIL-PRF-83282 and MIL-PRF-87257, provided excellent protection against rusting during storage.

     

Structural and dielectric behavior of Pb(Mn1/4Zn1/4W1/2)O3 ceramics

Polycrystalline complex perovskite compound of Pb(Mn_1/4Zn_1/4W_1/2)O₃ (PMZW) was prepared from a solid-state reaction technique. X-ray powder diffraction (XRD) study suggests the formation of single-phase compound in orthorhombic structure at room temperature. Dielectric studies as a function of temperature (305–573 K) yielded a small dielectric anomaly at 477 K. Variation of dc resistivity as a function of temperature suggests that the compound behaves as the negative temperature coefficient resistors (NTCR). Proper hysteresis loop could not be observed to confirm the ferroelectric behavior.     

Electrochemical Corrosion and Impedance Studies of Porous Ti–xNb–Ag Alloy in Physiological Solution

Porous titanium (Ti) and its alloys are promising materials for orthopedic applications due to their low elastic modulus, high strength, excellent corrosion resistance, and biocompatibility. In this study, the porous Ti–xNb–5Ag (x = 25, 30 and 35 wt%) alloys were synthesized using the powder metallurgy approach. The effects of Nb content on the porosity, mechanical properties, and electrochemical corrosion behavior of the alloys were investigated. XRD analysis revealed that the porous alloys mainly consist of α-Ti, β-Ti, intermetallic compound (Ti₄Nb), and oxides of TiO₂ and NbO phases. Porous alloys possess the porosity ranging from 57 to 65%, due to the addition of NH₄HCO₃ (45 wt%). Increase in Nb content lead to a reduction in the elastic modulus and compression strengths of the sintered porous Ti–xNb–5Ag alloys. All three developed porous Ti–xNb–5Ag alloys show the optimum combination of elastic modulus and compression strength, which is suitable for orthopedic applications. These porous alloys exhibit excellent electrochemical corrosion resistance in the simulated body fluids, and the samples having low porosity exhibit higher corrosion resistance than high-porosity samples.