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101.
Cellular DNA breakage by soy isoflavone genistein and its methylated structural analogue biochanin A
Mohd Fahad Ullah Uzma Shamim Sarmad Hanif Asfar S. Azmi Sheikh M. Hadi 《Molecular nutrition & food research》2009,53(11):1376-1385
Epidemiological studies have indicated that populations with high isoflavone intake through soy consumption have lower rates of breast, prostate, and colon cancer. The isoflavone polyphenol genistein in soybean is considered to be a potent chemopreventive agent against cancer. In order to explore the chemical basis of chemopreventive activity of genistein, in this paper we have examined the structure–activity relationship between genistein and its structural analogue biochanin A. We show that both genistein and its methylated derivative biochanin A are able to mobilize nuclear copper in human lymphocyte, leading to degradation of cellular DNA. However, the relative rate of DNA breakage was greater in the case of genistein. Further, the cellular DNA degradation was inhibited by copper chelator (neocuproine/bathocuproine) but not by compounds that specifically bind iron and zinc (desferrioxamine mesylate and histidine, respectively). We also compared the antioxidant activity of the two isoflavones against tert‐butylhydroperoxide‐induced oxidative breakage in lymphocytes. Again genistein was found to be more effective than biochanin A in providing protection against oxidative stress induced by tert‐butylhydroperoxide. It would therefore appear that the structural features of isoflavones that are important for antioxidant properties are also the ones that contribute to their pro‐oxidant action through a mechanism that involves redox cycling of chromatin‐bound nuclear copper. 相似文献
102.
Gohar Ayoub Javaid Akhtar Maham Rana Muhammad Qasim Naseer Sheikh 《Energy Sources, Part A: Recovery, Utilization, and Environmental Effects》2018,40(1):100-107
Three biomasses like Eucalyptus, Azadirachata, and Ficus religiosa were torrefied to investigate the effect of temperature and residence time was investigated on torrefied biomasses for yield, volatile matter, fixed carbon, ash, and Gross calorific value (GCV). Thermogravimetric analysis and Hardgrove grindability index (HGI) of three torrefied samples were studied at optimum conditions of temperature and residence time. According to the results, an optimum temperature was found to be 260°C at 45 min residence time. A maximum GCV of torrefied biomasses of Eucalyptus, Azadirachata, and F. religiosa were found to be 4,301, 3,190, and 3,278 kcal/kg, respectively. According to thermogravimetric analyzer results, the Azadirachata has shown higher weight loss compared to Eucalyptus and F. religiosa during thermogravimetirc study. The weight loss rate for Azadirachata was maximum to nearly 12.8%/min compared to 6.11 and 5.12%/min for Eucalyptus and F. religiosa, respectively. The order of reactivity based on mean reactivity and combustion characterization factor was found to be Azadirachata indica > Eucalyptus > F. religiosa. According to HGI results, Eucalyptus, Azadirachata, and F. religiosa have shown HGI values of 71, 60.7, and 81.7, respectively. The results of this study could be useful for the energy recovery schemes in the country. 相似文献
103.
The morphology, rheology, and mechanical properties of the dynamically vulcanized thermoplastic elastomer based on EPDM/PP (60/40, w:w) containing 5, 10, and 20% of cellulose short fiber were studied. From the results it was found that addition of 5% of cellulose fiber has no significant effect on the maximum torque associated with the dynamic vulcanization stage, while higher concentration of fibers decreased the maximum torque. These results were explained in terms of influence of cellulose fibers on the extent of agglomeration formed between the cured rubber particles. The results of tensile test performed on the samples showed that incorporation of 5% of cellulose fibers into the sample has an increasing effect on elongation at break with no appreciable change in tensile behavior. However increasing the cellulose fibers content of the sample to 20% had a remarkable change in tensile properties of the sample resulting in a toughened plastic type behavior. The melt elasticity of the sample containing 5% of cellulose fibers particularly at low shear rate range was found to be lower than that of the sample without fibers indicating the role of fibers in weakening of cured rubber particle agglomeration. This was supported with the results of the relaxation time distribution H(λ) and scanning electron microscopy of the samples. POLYM. COMPOS., 2009. © 2008 Society of Plastics Engineers 相似文献
104.
105.
Muzafar A. Kanjwal Nasser A. M. Barakat Faheem A. Sheikh Myung Seob Khil Hak Yong Kim 《Journal of Materials Science》2008,43(16):5489-5494
In this work, nanobelt mats consisting of three potential metal oxides have been produced using the electrospinning technique.
An aqueous solution of cobalt acetate tetra-hydrate, copper acetate mono-hydrate, and manganese acetate tetra-hydrate was
mixed with poly(vinyl alcohol) solution to prepare a sol–gel which was electrospun at 20 kV. The obtained nanofiber mats have
been vacuously dried at 80 °C for 24 h and then calcined in air atmosphere at different temperatures and soaking times. The
utilized physiochemical characterizations have affirmed that nanobelts composed of three oxides (Co3O4, CuO, and MnO2) can be prepared by calcination at a temperature of 600 °C for 1 h. High-resolution transmission electron microscope and
selected area electron pattern images revealed good crystallinity for the synthesized nanobelts. 相似文献
106.
Bhanu Priya Kilari Priti Mudgil Sheikh Azimullah Nidhi Bansal Shreesh Ojha Sajid Maqsood 《Journal of dairy science》2021,104(2):1304-1317
This study investigated the effect of camel milk protein hydrolysates (CMPH) at 100, 500 and 1,000 mg/kg of body weight (BW) for 8 wk on hyperglycemia, hyperlipidemia, and associated oxidative stress in streptozotocin-induced diabetic rats. Body weights and fasting blood glucose levels were observed after every week until 8 wk, and oral glucose tolerance test (OGTT) levels and biochemical parameters were evaluated after 8 wk in blood and serum samples. Antioxidant enzyme activity and lipid peroxidation in the liver were estimated, and histological examination of the liver and pancreatic tissues was also conducted. Results showed that CMPH at 500 mg/kg of BW [camel milk protein hydrolysate, mid-level dosage (CMPH-M)] exhibited potent hypoglycemic activity, as shown in the reduction in fasting blood glucose and OGTT levels. The hypolipidemic effect of CMPH was indicated by normalization of serum lipid levels. Significant improvement in activity of superoxide dismutase and catalase, and reduced glutathione levels were observed, along with the attenuation of malondialdehyde content in groups fed CMPH, especially CMPH-M, was observed. Decreased levels of liver function enzymes (aspartate aminotransferase and alanine aminotransferase) in the CMPH-M group was also noted. Histology of liver and pancreatic tissue displayed absence of lipid accumulation in hepatocytes and preservation of β-cells in the CMPH-M group compared with the diabetic control group. This is the first study to report anti-hyperglycemic and anti-hyperlipidemic effect of CMPH in an animal model system. This study indicates that CMPH can be suggested for its therapeutic benefits for hyperglycemia and hyperlipidemia, thus validating its use for better management of diabetes and associated comorbidities. 相似文献
107.
Aspergillus niger was subjected to UV radiation and chemical mutagenesis to develop its hyper-producing mutants for enhanced citric acid production. Ethyl methane sulfonate (EMS) and Ethidium bromide (EB) were used for chemical mutagenesis of Aspergillus niger. UV, and chemically treated mutants of Aspergillus niger were identified by using 2-deoxy, D-glucose as selective marker. The selected mutants were cultured in solid state fermentation (SSF) of sugarcane molasses medium (10%) using corn cobs, banana stalk, sugarcane bagasse, wheat straw, and wheat bran as carrier substrates. After pH adjustment and sterilization, the triplicate flasks were inoculated with 5 mLof homogenous spore suspensions of selected mutants of A. niger and the flasks were subjected to SSF under still culture conditions. The mutant EB-3 (treated with 1 mg/mL ethidium bromide for 120 min) giving highest citric acid yield (64.2 mg/mL) in 72 h was selected as hyper-producing mutant. Citric acid production process using EB-3 mutant was then optimized to enhance citric acid production by the mutant in SSF. Aspergillus niger EB-3 mutant could produce 67.72 mg/mL citric acid in 72 h using banana stalks as support material under optimum conditions of pH (pH 6), incubation temperature (35°C) and inoculum size (5 mL) in SSF. 相似文献
108.
Pakistan energy situation is seriously troubling today due to lack of careful planning and implementation of its energy policies. To avoid the worse situation in the years ahead, the country will have to exploit its huge natural renewable resource. In this paper a review is being presented about renewable energy resource potential available in the country to be exploited for useful and consistent energy supplies. On average solar global insolation 5–7 kWh/m2/day, wind speed 5–7.5 m/s, Biogas 14 million m3/day, microhydel more than 600 MW (for small units) with persistency factor of more than 80% over a year exist in the country. Solar and wind maps are presented along with identification of hot spring sites as resource of geothermal energy. The research results presented in this paper are not only useful for government policy makers, executing agencies but also for private sector national and international agencies and stake holders who want to invest in Pakistan for renewable energy projects or business. 相似文献
109.
Joanna L. Ryszkowska Monika Auguścik Ann Sheikh Aldo R. Boccaccini 《Composites Science and Technology》2010
Five types of solid and porous polyurethane composites containing 5–20 wt.% of Bioglass® inclusions were synthesized. Porous structures were fabricated by polymer coagulation combined with the salt-particle leaching method. In-vitro bioactivity tests in simulated body fluid (SBF) were carried out and the marker of bioactivity, e.g. formation of surface hydroxyapatite or calcium phosphate layers upon immersion in SBF, was investigated. The chemical and physical properties of the solid and porous composites before and after immersion in SBF were evaluated using different techniques: Fourier Transform Infrared Spectroscopy (FTIR), Differential Scanning Calorimetry (DSC), Dynamic Mechanical Analysis (DMA) and Thermogravimetric Analysis (TGA). Moreover the surface structure and microstructure of the composites was characterised by Atomic Force Microscopy (AFM) and Scanning Electron Microscopy (SEM), respectively. Mercury intrusion porosimetry, SEM and microtomography (μCT) were used to determine pore size distribution and porosity. The fabricated foams exhibited porosity >70% with open pores of 100–400 μm in size and pore walls containing numerous micropores of <10 μm. This pore structure satisfies the requirements for bone tissue engineering applications. The effects of Bioglass® addition on microstructure, mechanical properties and bioactivity of polyurethane scaffolds were evaluated. It was found that composite foams showed a higher storage modulus than neat polyurethane foams. The high bioactivity of composite scaffolds was confirmed by the rapid formation of hydroxyapatite on the foam surfaces upon immersion in SBF. 相似文献
110.
A. F. M. Arif A. K. Sheikh S. Z. Qamar K. M. Al-Fuhaid 《Materials and Manufacturing Processes》2001,16(5):701-716
Prediction of extrusion pressure, especially in the case of complex die geometries, is an area of continued research interest. Die complexity, usually defined by “shape factor” (the ratio of the perimeter to the cross-sectional area of the profile), critically affects the flow of metal and the pressure required to extrude a given product. Applied strain rate (related directly to the ram speed of the extrusion press) also alters the product quality significantly. The current paper presents results of an ongoing study about effects of ram speed and die profile on extrusion pressure. Experiments were conducted using dies of different complexity to track the effects of ram speed variation and changing die profiles on extrusion pressure. Al-6063, the most popular commercial variety of structural aluminum, was used as the billet material for all experiments. 相似文献