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81.
After a series of investigations on the durable flame‐retardant finishes, it was thought to be important to study these durable flame‐retardant finished materials from the thermal analytical standpoint. Accordingly, cotton fabric was finished with N‐methylol dialkyl phosphonopropionamide (Pyrovatex C) by thermofixation and tetrakis (hydroxymethyl) phosphonium sulfate (THPS) precondensate by ammonia cure (Proban), as well as with THPS monomer by heat cure under various conditions, and subjected to the thermogravimetry (TG) to observe thermal degradation behaviors and obtain apparent activation energy (Ea). TG curves of Proban‐finished samples showed the largest shift to lower temperatures with a steep slope; thermofixed THPS‐finished sample gave a smaller shift with similar steep slope, whereas Pyrovatex‐finished samples exhibited a similar shift but with a gradual slope. Ea versus residual ratio curves led us to conclude that C N bond‐rich Proban polymer requires the highest Ea and decomposes with considerable rapidity, whereas ethylene‐bond‐rich Pyrovatex‐finished samples with melamine crosslinking decompose gradually with the lowest Ea. As for the relationship between flame retardance and Ea distribution in the process of thermal degradation, typical differences among the above three kinds of finished samples were found, which are compared and discussed. © 1999 John Wiley & Sons, Inc. J Appl Polym Sci 71: 975–987, 1999 相似文献
82.
Muroya A Nakano R Ohtani N Haruki M Morikawa M Kanaya S 《Journal of Bioscience and Bioengineering》2002,93(2):170-175
The gene encoding ribonuclease HII from Bacillus stearothermophilus was cloned and expressed in Escherichia coli. The overproduced protein, Bst-RNase HII, was purified and biochemically characterized. Bst-RNase HII, which consists of 259 amino acid residues, showed the highest amino acid sequence identity (50.2%) to Bacillus subtilis RNase HII. Like B. subtilis RNase HII, it exhibited Mn2+-dependent RNase H activity. It was, however, more thermostable than B. subtilis RNase HII. When the Bst-RNase HII amino acid sequence is compared with that of Thermococcus kodakaraensis RNase HII, to which it shows 29.8% identity, 30 residues are observed to be truncated from the C-terminus and there is an extension of 71 residues at the N-terminus. The C-terminal truncation results in the loss of the alpha9 helix, which is rich in basic amino acid residues and is therefore important for substrate binding. A truncated protein, Delta59-Bst-RNase HII, in which most of the N-terminal extension was removed, completely lost its RNase H activity. Surface plasmon resonance analysis indicated that this truncated protein did not bind to the substrate. These results suggest that the N-terminal extension of Bst-RNase HII is important for substrate binding. Because B. subtilis RNase HII has an N-terminal extension of the same length and these extensions contain a region in which basic amino acid residues are clustered, the Bacillus enzymes may represent a novel type of RNase H which possesses a substrate-binding domain at the N-terminus. 相似文献
83.
Preparation of a temperature‐responsive smart paper using a molecularly imprinted polymer and lipid bimolecular membrane
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A smart paper that provided sustained release of benzalkonium chloride (BKC) at a set temperature was prepared. Filter paper impregnated with an oil‐in‐water emulsion containing BKC, methyl methacrylate monomer, ethylene diamine, and 2,2‐azobis(2,4‐dimethylvaleronitrile) was immersed in a cyclohexane solution of terephthaloyl chloride. A polyamide film containing the BKC MIP was formed on the paper surface via an interfacial polymerization reaction. Dimethlydistearylammonium bromide (DDB) was then coated over the polyamide film. The interfacial polymerization reaction eliminated the need for preparation of microcapsules of BKC or coating of the paper with a binder, which are methods commonly used for preparation of smart papers. Release of BKC from the paper was studied, and the temperature responses obtained with the DDB coating were evaluated. The smart paper with the BKC MIP and DDB coating showed sustained release of BKC, and this function was obtained in response to a certain temperature. © 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2017 , 134, 44530. 相似文献
84.
Naoki Maeda Yasuo Kokai Seiji Ohtani Takahiko Hada Hiromi Yoshida Yoshiyuki Mizushina 《Food chemistry》2009
The glycoglycerolipid fraction from spinach was purified, and this fraction was used to clarify bioactive functions, such as inhibition of DNA polymerase activity and cancer cell growth. The effects of the spinach glycoglycerolipid fraction on preventive and curative antitumor activities, and acute toxicity were investigated. After oral administration of 20 mg/kg of the glycoglycerolipid fraction for 2 weeks as preliminary medication, colon tumor growth was delayed, and the protein expression level of proliferating cell nuclear antigen (PCNA) was decreased in tumor tissue. Five days after tumor cell implantation, oral administration of, not only 70 mg/kg of the glycoglycerolipid fraction, but also the γ-cyclodextrin (CD) glycoglycerolipid fraction complex, for 4 weeks, suppressed sarcoma formation with no adverse reactions in mice. In the acute toxicity test, 2000 mg/kg of orally administered glycoglycerolipid fraction did not show evident toxicity. Hence, these results suggest that the spinach glycoglycerolipid fraction is a safe and effective anticancer bioactive agent and/or food material. 相似文献
85.
Sato T Yamada Y Ohtani Y Mitsui N Murasawa H Araki S 《Journal of Bioscience and Bioengineering》2001,91(1):16-20
Menaquinone-7 (MK-7) is a highly bioactive homologue of vitamin K. We obtained a diphenylamine-resistant mutant strain D200-41 from Bacillus subtilis strain MH-1 which was isolated from fermented soybeans, natto. The mutant strain exhibited decreased production of MK-6. Using strain D200-41, efficient production of MK-7 was achieved. We found that, compared with an agitated and aerated culture, production of MK-7 was increased by static culture. The sporulation of the cells progressed more slowly in a static culture than in an agitated culture. The maximum concentration of MK reached about 60 mg/l in a medium containing 10% soybean extract, 5% glycerol, 0.5% yeast extract and 0.05% K2HPO4 (pH 7.3) when D200-41 cells as well as MH-1 cells were statically cultured at 45 degrees C for 5 d after being cultured with shaking at 37 degrees C for 1 d. 相似文献
86.
Bonamali Pal Tsukasa Torimoto Kentaro Iwasaki Tamaki Shibayama Heishichiro Takahashi Bunsho Ohtani 《Journal of Applied Electrochemistry》2005,35(7-8):751-756
Photocatalytic deposition of gold (Au) and silver (Ag) nanoparticles was investigated using jingle-bell-shaped silica (SiO2)-coated cadmium sulfide (CdS) nanoparticles (SiO2/CdS), which each had a void space between the CdS core and SiO2 shell, as a photocatalyst. A size-selective photoetching technique was used to prepare the jingle bell nanostructure of SiO2/CdS. Nanoparticles of Au and Ag were deposited by irradiation of the photoetched SiO2/CdS in the presence of the corresponding metal complexes under deaerated conditions. Chemical etching of Au-deposited particles
enabled the selective removal of CdS without any influence on the surface-plasmon absorption of Au. TEM analyses of the resulting
particles suggested that some particles were encapsulated in hollow SiO2 particles, while other Au particles were deposited on the outer surface of the SiO2 shell. Emission spectra of the photoetched SiO2/CdS showed that the metal deposition developed a broad emission with a peak around 650 nm originating from surface defect
sites, the degree being dependent on the kind of metal nanoparticles and their amount of deposition. This fact can be explained
by the formation of metal–CdS binary nanoparticles having defect sites at the interface between metal and CdS. 相似文献
87.
88.
We determined the changes in neuropeptide Y (NPY) mRNA expression of the arcuate nucleus (ARC) in sham-operated (SHAM) and bilaterally ovariectomized (OVX) rats with estradiol (E2) supplement. Ovariectomy increases body weight gain for 3 weeks, accompanied by an increase of daily food intake. Ovariectomy significantly reduced serum corticosterone levels. E2 supplement reversed the effects of ovariectomy on body weight gain, food intake and serum corticosterone levels. Ovariectomy significantly increased NPY mRNA expression in the ARC. E2 supplement decreased NPY mRNA expression in the ARC of OVX rats. The present findings indicated that hypothalamic NPY mRNA expression, which involves the regulation of feeding behavior, are in parallel with circulating estrogen levels. Hypothalamic NPY mRNA expression may be important in the induction of hyperphagia after the withdrawal of estrogen by bilateral ovariectomy. 相似文献
89.
T. Tanabe K. Suto T. Kimura T. Ohtani J. Nishizawa 《Materials Science in Semiconductor Processing》2003,6(5-6):445-447
A GaPAs waveguide with GaP cladding layers, has been fabricated in only two steps of LPE growth. One-way optical loss of the fabricated GaPAs–GaP waveguide is 12% mm−1 (α=1.9 cm−1). The free carrier concentration of the GaPAs layer is 1×1016 cm−3 (α=0.05 cm−1). The backward spontaneous Raman spectrum from the GaPAs waveguide shows a GaP-like longitudinal optical (LO) phonon line. The LO band is intensified and shifted to a higher frequency compared to the LO phonon of GaP bulk crystal. 相似文献
90.
Nano-scale imaging of chromosomes and DNA by scanning near-field optical/atomic force microscopy 总被引:2,自引:0,他引:2
Yoshino T Sugiyama S Hagiwara S Fukushi D Shichiri M Nakao H Kim JM Hirose T Muramatsu H Ohtani T 《Ultramicroscopy》2003,97(1-4):81-87
Nano-scale structures of the YOYO-1-stained barley chromosomes and lambda-phage DNA were investigated by scanning near-field optical/atomic force microscopy (SNOM/AFM). This technique enabled precise analysis of fluorescence structural images in relation to the morphology of the biomaterials. The results suggested that the fluorescence intensity does not always correspond to topographic height of the chromosomes, but roughly reflects the local amount and/or density of DNA. Various sizes of the bright fluorescence spots were clearly observed in fluorescence banding-treated chromosomes. Furthermore, fluorescence-stained lambda-phage DNA analysis by SNOM/AFM demonstrated the possibility of nanometer-scale imaging for a novel technique termed nano-fluorescence in situ hybridization (nano-FISH). Thus, SNOM/AFM is a powerful tool for analyzing the structure and the function of biomaterials with higher resolution than conventional optical microscopes. 相似文献