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11.
The effect of the N-linked glycans on structural features and physicochemical functions of soybean β-conglycinin homotrimers 总被引:2,自引:0,他引:2
Nobuyuki Maruyama Mohamad Ramlan Mohamed Salleh Koji Takahashi Kazuhiro Yagasaki Hideyuki Goto Naho Hontani Shuko Nakagawa Shigeru Utsumi 《Journal of the American Oil Chemists' Society》2002,79(2):139-144
β-Conglycinin is a trimeric protein consisting of three subunits, α,α′,and β, which are N-glycosylated. The α and α′ subunits
contain extension regions in addition to core regions common to all subunits. We purified homogeneous trimers consisting of
only α, α′, or β from mutant soybean cultivars containing β-conglycinin lacking one or two subunits: α homotrimers from an
α′-lacking mutant, α′ homotrimers from an α-lacking mutant, and β homotrimers from an α-and α′-lacking mutant. Structural
features and physicochemical functions of the three homotrimers were examined and compared with those of recombinant homotrimers
having no N-linked glycans. The native homotrimers have secondary structures very similar to those of the recombinant ones.
In analogy with the recombinant homotrimers, the native ones exhibit different thermal stabilities from one another (β>α′>α),
and the native α and α′ homotrimers exhibit better solubility, emulsifying ability, and heat-induced association than the
native β homotrimer. Further, the N-linked glycans contribute to solubilities of the three subunits at low ionic strength
(μ=0.08) and to the emulsifying ability of the native β homotrimer. N-Linked glycans also prevent heat-induced associations
of the native α and α′ homotrimers but do not contribute to the secondary structure and the thermal stability of β-conglycinin. 相似文献
12.
The set of firing rates of the presynaptic excitatory and inhibitory neurons constitutes the input signal to the postsynaptic neuron. Estimation of the time-varying input rates from intracellularly recorded membrane potential is investigated here. For that purpose, the membrane potential dynamics must be specified. We consider the Ornstein-Uhlenbeck stochastic process, one of the most common single-neuron models, with time-dependent mean and variance. Assuming the slow variation of these two moments, it is possible to formulate the estimation problem by using a state-space model. We develop an algorithm that estimates the paths of the mean and variance of the input current by using the empirical Bayes approach. Then the input firing rates are directly available from the moments. The proposed method is applied to three simulated data examples: constant signal, sinusoidally modulated signal, and constant signal with a jump. For the constant signal, the estimation performance of the method is comparable to that of the traditionally applied maximum likelihood method. Further, the proposed method accurately estimates both continuous and discontinuous time-variable signals. In the case of the signal with a jump, which does not satisfy the assumption of slow variability, the robustness of the method is verified. It can be concluded that the method provides reliable estimates of the total input firing rates, which are not experimentally measurable. 相似文献
13.
The time histogram is a fundamental tool for representing the inhomogeneous density of event occurrences such as neuronal firings. The shape of a histogram critically depends on the size of the bins that partition the time axis. In most neurophysiological studies, however, researchers have arbitrarily selected the bin size when analyzing fluctuations in neuronal activity. A rigorous method for selecting the appropriate bin size was recently derived so that the mean integrated squared error between the time histogram and the unknown underlying rate is minimized (Shimazaki & Shinomoto, 2007 ). This derivation assumes that spikes are independently drawn from a given rate. However, in practice, biological neurons express non-Poissonian features in their firing patterns, such that the spike occurrence depends on the preceding spikes, which inevitably deteriorate the optimization. In this letter, we revise the method for selecting the bin size by considering the possible non-Poissonian features. Improvement in the goodness of fit of the time histogram is assessed and confirmed by numerically simulated non-Poissonian spike trains derived from the given fluctuating rate. For some experimental data, the revised algorithm transforms the shape of the time histogram from the Poissonian optimization method. 相似文献
14.
Kikuta K Tochigi N Saito S Shimoda T Morioka H Toyama Y Hosono A Suehara Y Beppu Y Kawai A Hirohashi S Kondo T 《Proteomics. Clinical applications》2010,4(5):560-567
Purpose : We aimed to identify novel chemotherapy responsiveness biomarkers for osteosarcoma (OS) by investigating the global protein expression profile of 12 biopsy samples from OS patients. Experimental design : Six patients were classified as good responders and six as poor responders, according to the Huvos grading system. The protein expression profiles obtained by 2‐D DIGE consisted of 2250 protein spots. Results : Among them, we identified 55 protein spots whose intensity was significantly different (Bonferroni adjusted p‐value<0.01) between the two patient groups. Mass spectrometric protein identification demonstrated that the 55 spots corresponded to 38 distinct gene products including peroxiredoxin 2 (PRDX 2). Use of a specific antibody against PRDX 2 confirmed the differential expression of PRDX 2 between good and poor responders, while PRDX 2 levels as measured by Western blotting correlated highly with their corresponding 2‐D DIGE values. The predictive value of PRDX 2 expression was further confirmed by examining an additional four OS cases using Western blotting. Conclusions and clinical relevance : These results establish PRDX 2 as a candidate for chemotherapy responsiveness marker in OS. Measuring PRDX 2 in biopsy samples before treatment may contribute to more effective management of OS. 相似文献
15.
A quantum-inspired evolutionary algorithm (QEA) is proposed as a stochastic algorithm to perform combinatorial optimization
problems. The QEA is evolutionary computation that uses quantum bits and superposition states in quantum computation. Although
the QEA is a coarse-grained parallel algorithm, it involves many parameters that must be adjusted manually. This paper proposes
a new method, named pair swap, which exchanges each best solution information between two individuals instead of migration
in the QEA. Experimental results show that our proposed method is a simpler algorithm and can find a high quality solution
in the 0-1 knapsack problem.
This work was presented in part at the 12th International Symposium on Artificial Life and Robotics, Oita, Japan, January
25–27, 2007 相似文献
16.
基于三维人脸成像系统的复数域人脸识别方法 总被引:1,自引:1,他引:1
三维人脸识别是模式识别和人工智能领域的研究热点。提出了一种利用人脸图像的二维灰度信息和三维深度信息进行人脸识别的算法。首先利用相关型图像传感器构成三维实时人脸成像系统,并将获得的三维人脸物理数据用复数形式表达。再将特征脸(Eigenface)方法和Fisherface方法拓展到复数域,提出了复数域特征脸方法和复数域Fisherface方法。改进的复数域人脸识别方法在三维实时成像系统的人脸数据库中进行的人脸识别实验表明,复数域的三维人脸识别方法明显优于传统的人脸识别方法。 相似文献
17.
Adhesion between polymer surface modified by graft polymerization and tissue during surgery using an ultrasonically activated scalpel device 下载免费PDF全文
Kwangwoo Nam Takuya Iwata Tsuyoshi Kimura Hiroki Ikake Shigeru Shimizu Toru Masuzawa Akio Kishida 《应用聚合物科学杂志》2014,131(20)
In the field of surgery, achieving adhesion between a polymer implant and tissue poses a challenge considering that suturing is not appropriate for the stability of such implants. An ultrasonically activated scalpel that generates heat by mechanical vibration and promotes adhesion between a polymer implant and native tissue by pressing the two materials together has very good potential for application in the field. To determine the type of polymer that is suitable for the purpose, we investigated polyethylene (PE) and polystyrene (PS) films, the surfaces of which were activated by corona discharge. Graft polymerization was then performed on the corona‐treated surfaces to vary their properties. The corona‐treated PE and PS films grafted with poly(acrylic acid), poly(methacrylic acid), poly(vinyl benzylacrylic acid), and poly(hydroxylethyl acrylate), respectively, adhered to the tissue when the ultrasonically activated scalpel was applied. The heat generated by the mechanical vibration and the applied pressure enabled the carboxyl or hydroxyl groups to bond with the proteins in the extracellular matrix. We therefore concluded that it was possible to integrate this technique in the development of new types of polymer devices that could be stably implanted in a living body. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014 , 131, 40885. 相似文献
18.
Invert traps have been successfully used to collect sediments at convenient locations within the sewer network, where large volumes of solids can be stored. In the present study, experiments have been performed in 15 cm wide and 5 m long channel for the measurement of retention ratios of five different invert trap configurations (namely, rectangular, trapezoidal with one side vertical, trapezoidal, trapezoidal with rectangular base, rectangular with trapezoidal base) having top width of 32 cm and depth of 28 cm with slots of three different sizes (namely, 5, 9 and 15 cm) for the flow of seven different sediment types (namely, two types of sand, glass beads and four types of plastic beads) at different flow rates for each trap. The flow rates selected in present study cover entire range of flow rate expected in channels during dry weather flow and monsoon. Flow field and retention ratio predictions for each invert trap configuration have been carried out using CFD modeling with the help of FLUENT software using Renormalization Group (RNG) k–ε along with Discrete Phase Model (DPM). The simulation results are capable of showing particle trajectories, the effect of flow rate and trap geometry on the flow patterns, developed within the trap. Based on CFD modeling and experimental measurements, it is concluded that the invert trap having rectangular shape with trapezoidal base is the most efficient trap configuration with highest sediment retention ratio. 相似文献
19.
Mary Ann O. Torio Takafumi Itoh Roberta N. Garcia Nobuyuki Maruyama Shigeru Utsumi Evelyn Mae Tecson-Mendoza 《Food research international (Ottawa, Ont.)》2012,45(1):277-282
Sulfhydryl groups and disulfide bond were introduced in mungbean's major storage protein, 8Sα globulin, by protein engineering to improve structural stability and functional properties. Five modified proteins or mutants (F59C, I99C, A213C, one free sulfhydryl group; I99C/A213, one disulfide bridge; F59C/I99C/A213C, one free sulfhydryl group and one disulfide linkage) were expressed in Escherichia coli at a yield similar to that of the unmodified protein or wild type (WT) in soluble form (38%). The number of introduced groups in the mutants was confirmed by Ellman analysis. Mutant and WT proteins exhibited similar elution patterns on gel filtration indicating their trimeric native conformation. Mutants had 2 to 3.8 °C higher Tm values than WT and were digested by chymotrypsin at 52–58% in 60 min but exhibited different digestion patterns. All mutants showed greater hardness of heat-induced gels than WT, especially I99C/A213C and F59C/I99C/A213C. Results indicate the improved structural stability of the modified 8Sα globulin. 相似文献
20.
Arkady Zgonnikov Ihor Lubashevsky Shigeru Kanemoto Toru Miyazawa Takashi Suzuki 《Journal of the Royal Society Interface》2014,11(99)
Understanding how humans control unstable systems is central to many research problems, with applications ranging from quiet standing to aircraft landing. Increasingly, much evidence appears in favour of event-driven control hypothesis: human operators only start actively controlling the system when the discrepancy between the current and desired system states becomes large enough. The event-driven models based on the concept of threshold can explain many features of the experimentally observed dynamics. However, much still remains unclear about the dynamics of human-controlled systems, which likely indicates that humans use more intricate control mechanisms. This paper argues that control activation in humans may be not threshold-driven, but instead intrinsically stochastic, noise-driven. Specifically, we suggest that control activation stems from stochastic interplay between the operator''s need to keep the controlled system near the goal state, on the one hand, and the tendency to postpone interrupting the system dynamics, on the other hand. We propose a model capturing this interplay and show that it matches the experimental data on human balancing of virtual overdamped stick. Our results illuminate that the noise-driven activation mechanism plays a crucial role at least in the considered task, and, hypothetically, in a broad range of human-controlled processes. 相似文献