Epitope mapping of a 95 kDa antigen in complex with antibody by solution-phase amide backbone hydrogen/deuterium exchange monitored by Fourier transform ion cyclotron resonance mass spectrometry

The epitopes of a homohexameric food allergen protein, cashew Ana o 2, identified by two monoclonal antibodies, 2B5 and 1F5, were mapped by solution-phase amide backbone H/D exchange (HDX) coupled with Fourier transform ion cyclotron resonance mass spectrometry (FTICR MS) and the results were compared to previous mapping by immunological and mutational analyses. Antibody 2B5 defines a conformational epitope, and 1F5 defines a linear epitope. Intact murine IgG antibodies were incubated with recombinant Ana o 2 (rAna o 2) to form antigen-monoclonal antibody (Ag-mAb) complexes. mAb-complexed and uncomplexed (free) rAna o 2 were then subjected to HDX. HDX instrumentation and automation were optimized to achieve high sequence coverage by protease XIII digestion. The regions protected from H/D exchange upon antibody binding overlap and thus confirm the previously identified epitope-bearing segments: the first extension of HDX monitored by mass spectrometry to a full-length antigen-antibody complex in solution.     

Hybrid inorganic membrane technology shows promise in gas processing

This paper examines how the use of novel hybrid inorganic membrane technology is enabling natural gas processing and the production of high density hydrogen without any impurities.     

Generalisation of the Routh-Hurwitz criterion and its applications

A generalisation of the Routh-Hurwitz stability criterion for testing the presence of unstable roots of a polynomial with complex coefficients is presented. Numerical examples are given to illustrate the application of the generalisation.     

Carry-over round robin: a simple cell scheduling mechanism for ATMnetworks

We propose a simple mechanism named carry-over round robin (CORR) for scheduling cells in asynchronous transfer mode networks. We quantify the operational complexity of CORR scheduling and show that it is comparable to that of a simple round-robin scheduler. We then show that, albeit its simplicity, CORR is very competitive with much more sophisticated and significantly more complex scheduling disciplines in terms of performance. We evaluate the performance of CORR using both analysis and simulation, We derive analytical bounds on the worst case end-to-end delay achieved by a CORR scheduler for different traffic arrival patterns. Using traffic traces from MPEG video streams, we compare the delay performance of CORR with that of packet-by-packet generalized processor sharing (PGPS) and stop-and-go (SG). Our results show that, in terms of delay performance, CORR compares favorably with both PGPS and SG. We also analyze the fairness properties of CORR and show that it achieves near perfect fairness     

Rantest: a fortran iv program for testing randomness of uniform pseudorandom numbers

Computer-generated random numbers form the basis of most Monte-Carlo simulations and similar work. A FORTRAN program—RANTEST—was written that performs various statistical and empirical tests of randomness on uniform (0, 1) pseudorandom numbers.     

Intrinsic viscosity of polymers and biopolymers measured by microchip

Intrinsic viscosity provides insight to molecular structure and interactions in solution. A new microchip method is described for fast and accurate measurements of viscosity and intrinsic viscosity of polymer and biopolymer solutions. Polymer samples are diluted with solvent in the microfluidic chip by imposing pressure gradients across the channel network. The concentration and flow dilutions of the polymer sample are calculated from the fluorescent signals recorded over a range of dilutions. The viscosities at various polymer dilutions are evaluated using mass and momentum balances in the pressure-driven microchannel flow. The technique is particularly important to many chemical, biological, and medical applications where sample is available in very small quantities. The intrinsic viscosity experiments were performed for three classes of polymer solutions: (a) poly(ethylene glycol), polymers with linear hydrocarbon chains; (b) bovine serum albumin, biopolymer chains with hydrophobic and hydrophilic amino acids, and (c) DNA fragments, biological macromolecules with double-stranded polymeric chains. The measured values of intrinsic viscosity agree remarkably well with the available data obtained using different methods. The data exhibit power law behavior for molecular weight as described by the Mark-Houwink-Sakurada equation. Experiments were performed to understand the effect of solvent quality and salt concentration on molecular conformations and the intrinsic viscosity of the polymers. This method offers a new way to study the conformational changes in proteins and DNA solutions in various buffer conditions such as pH, ionic strength, and surfactants. The effects of shear rate in the microchannel and mixing time on the accuracy and limitation of the measurement method are discussed.