Secreted HSP90α-LRP1 Signaling Promotes Tumor Metastasis and Chemoresistance in Pancreatic Cancer

The extracellular heat shock protein 90α (eHSP90α) has been reported to promote cancer cell motility. However, whether pancreatic cancer (PC) cells expressed membrane-bound or secreted HSP90α, as well as its underlying mechanism for PC progression, were still unclear. Our study demonstrated that the amounts of secreted HSP90α proteins were discrepant in multiple PC cells. In addition, highly invasive Capan-2 cells have a higher level of secreted HSP90α compared with those of less invasive PL45 cells. The conditioned medium of Capan-2 cells or recombinant HSP90α treatment stimulated the migration and invasion of PC cells, which could be prevented with a neutralizing anti-HSP90α antibody. Furthermore, secreted HSP90α promoted elements of epithelial–mesenchymal transition in PL45 cells, including increases in vimentin and Snail expressions, decreases in E-cadherin expression, and changes in cell shape towards a mesenchymal phenotype, but these phenomena were reversed by the anti-HSP90α antibody in Capan-2 cells. In addition, high levels of low-density lipoprotein receptor-related protein 1 (LRP1) were associated with worsened patient survival in pancreatic adenocarcinoma. We demonstrated LRP1 as a receptor of eHSP90α for its stimulatory role in metastasis, by activating the AKT pathway. In addition, silencing LRP1 enhanced the chemosensitivity to gemcitabine and doxorubicin in Capan-2 cells. Therefore, our study indicated that blocking secreted HSP90α underlies an aspect of metastasis and chemoresistance in PC.     

Molecular and Physiological Responses of Citrus sinensis Leaves to Long-Term Low pH Revealed by RNA-Seq Integrated with Targeted Metabolomics

Low pH-induced alterations in gene expression profiles and organic acids (OA) and free amino acid (FAA) abundances were investigated in sweet orange [Citrus sinensis (L.) Osbeck cv. Xuegan] leaves. We identified 503 downregulated and 349 upregulated genes in low pH-treated leaves. Further analysis indicated that low pH impaired light reaction and carbon fixation in photosynthetic organisms, thereby lowering photosynthesis in leaves. Low pH reduced carbon and carbohydrate metabolisms, OA biosynthesis and ATP production in leaves. Low pH downregulated the biosynthesis of nitrogen compounds, proteins, and FAAs in leaves, which might be conducive to maintaining energy homeostasis during ATP deprivation. Low pH-treated leaves displayed some adaptive responses to phosphate starvation, including phosphate recycling, lipid remodeling, and phosphate transport, thus enhancing leaf acid-tolerance. Low pH upregulated the expression of some reactive oxygen species (ROS) and aldehyde detoxifying enzyme (peroxidase and superoxidase) genes and the concentrations of some antioxidants (L-tryptophan, L-proline, nicotinic acid, pantothenic acid, and pyroglutamic acid), but it impaired the pentose phosphate pathway and V_E and secondary metabolite biosynthesis and downregulated the expression of some ROS and aldehyde detoxifying enzyme (ascorbate peroxidase, aldo-keto reductase, and 2-alkenal reductase) genes and the concentrations of some antioxidants (pyridoxine and γ-aminobutyric acid), thus disturbing the balance between production and detoxification of ROS and aldehydes and causing oxidative damage to leaves.     

Dynamic characteristic analysis of TBM tunnelling in mixed-face conditions

In this paper, by taking into account the periodically varying mesh stiffness in multiple pinions transmission and the speed–torque characteristics of variable frequency motor drives, the dynamic model for the revolving system of tunnel boring machine (TBM) has been established. Based on the soft ground/hard rock assumption of mixed-face conditions and the analysis of cutting force on each disc cutter and each drag bit, the time-varying excavation torque on the TBM cutterhead in mixed-face tunnelling is obtained. The dynamic excavation torque and cutterhead rotation speed are discussed and compared for TBM tunnelling in several typical mixed-face conditions, which are characterized by the area percentage of soft ground on the excavation face and the uniaxial compressive strength (UCS) of rock. The results show that the excavation torque may run up to a critical value and fluctuate greatly in extremely adverse excavation environments, which may lead to an unexpected TBM stoppage and even a catastrophic failure of the drive motor. To decrease the penetration per revolution in time through applying a lower advance velocity and a higher cutterhead revolution will significantly reduce the excavation torque and effectively avoid such situations.     

Termination detection for dynamically distributed systems with non-first-in-first-out communication

We propose a new algorithm for detecting termination of distributed systems. The algorithm works correctly whether the system is static or dynamic, whether the interprocess communication is synchronous or asynchronous, and whether the communication channels are first-in-first-out or not. The algorithm requires, in the worst case, O(nm) control messages in all, where n is the number of processes in the system and m is the total number of messages transmitted during the operation of the system. After the system terminates, the algorithm is able to detect the termination using only O(n) control messages; it is optimal if the system concerned is static.     

Discrimination of breast cancer by measuring prostate-specific antigen levels in women's serum

Prostate-specific antigen (PSA) has been reported to be a potential biomarker of breast cancer. Serum PSA of normal women is around 1 pg/mL, which is usually undetectable by current assay methods; thus an ultrasensitive measurement of PSA expression in women's serum is necessary to distinguish normal from malignant breast diseases. To enhance the sensitivity of conventional immunoassay technology for the detection of PSA in sera, we adopted a localized surface plasmon coupled fluorescence fiber-optic biosensor, which combines a sandwich immunoassay with the localized surface plasmon technique. The concentration of total PSA (t-PSA) (from 0.1 to 1000 pg/mL) in phosphate-buffered saline solution and the normalized fluorescence signal exhibit a linear relationship where the correlation coefficient is 0.9574. In addition, the concentration of additional t-PSA in 10-fold-diluted healthly women's serum across a similar range was measured. The correlation coefficient for this measurement is 0.9142. In clinical serum samples, moreover, the experimental results of t-PSA detection show that both the mean value and median of normalized fluorescence signals in the breast cancer group (155.2 and 145.7, respectively) are higher than those in the noncancer group (46.6 and 37.1, respectively). We also examined the receiver operating characteristic curve for t-PSA, and the area under the curve (AUC) is estimated to be 0.9063, the AUC being used to measure the performance of a test to correctly identify diseased and nondiseased subjects.     

噪声数据压缩器检测平台的PCM解码实现

为了提高数据的精度,便于收发端进行数据加工和处理、适应数字技术和大规模集成技术的飞速发展,研究了脉冲编码调制方式PCM（Pulse Code Modulation）。主要对噪声数据压缩器实时下传的PCM码进行解码,通过上位机读出数据进行测试,完成对系统进行功能验证。重点在硬件的功能实现上对其进行阐述,通过试验准确地接收到数据,实现PCM码流解码,从而证明本设计方案切实可行。     

Tiered bandwidth reservation scheme for multimedia wireless networks

It is important to provide quality of service (QoS) guarantees if we want to support multimedia applications over wireless networks. In this paper, considering the features of tiering in sectored cellular networks, we propose a novel scheme for bandwidth reservation to approach QoS provisioning. By predicting the movement of each connection, the reserving of bandwidth is only required in needful neighboring cells instead of in all neighboring cells. In addition, an admission control mechanism incorporated with bandwidth borrowing assists in distributing scarce wireless bandwidth in more adaptive way. Through mathematical analysis, we proof the advantages of tier‐based approach and the bound for the selection of tiered boundary. The simulation results also verify that our scheme can achieve superior performance than traditional schemes regarding no bandwidth reserving, fixed bandwidth reserving, and bandwidth borrowing in sectored cellular networks when performance metrics are measured in terms of the connection dropping probability (CDP), connection blocking probability (CBP), and bandwidth utilization (BU). Copyright © 2008 John Wiley & Sons, Ltd.     

Theoretical studies of CO hydrogenation to methanol over Cu, Pd, and Pt metals

Theoretical studies of CO hydrogenation to methanol over Cu, Pd, and Pt metals have been carried out using a quasi‐relativistic density‐functional method. The metal surface is simulated by a M₁₀ cluster model. Reaction energies for the elementary steps involved are determined. The activation energies are estimated by the analytic BOC‐MP formula. The results support that these metals are active in CO hydrogenation to methanol. The rate‐determining steps are shown to be different for the metals. The highest activation energies of reaction on the metals fall in the order Cu < Pd < Pt, which corresponds to the order of the catalytic activities of the metals in CO hydrogenation. This revised version was published online in July 2006 with corrections to the Cover Date.     

On uniform integrability and asymptotically risk-efficient sequential estimation

In the theory of asymptotically risk-efficient sequential estimation, one encounters uniform integrability of both positive and negative powers of stopping rules and moment convergence of randomly stopped statistics. We describe a simple approach to obtain these uniform integrability and moment convergence results, not only in the classical setting of i.i.d. observations but also for much more general stochastic sequences. We also use this approach to establish asymptotic risk efficiency of sequential estimators of means of stochastic sequences and to derive asymptotic approximations for the mean squared errors of ratio estimators.