Laura Damian  Cristian Cezar Login  Carolina Solomon  Cristina Belizna  Svetlana Encica  Laura Urian  Ciprian Jurcut  Bogdan Stancu  Romana Vulturar 《International journal of molecular sciences》2022,23(13)

Inclusion body myositis (IBM) is an acquired, late-onset inflammatory myopathy, with both inflammatory and degenerative pathogenesis. Although idiopathic inflammatory myopathies may be associated with malignancies, IBM is generally not considered paraneoplastic. Many studies of malignancy in inflammatory myopathies did not include IBM patients. Indeed, IBM is often diagnosed only after around 5 years from onset, while paraneoplastic myositis is generally defined as the co-occurrence of malignancy and myopathy within 1 to 3 years of each other. Nevertheless, a significant association with large granular lymphocyte leukemia has been recently described in IBM, and there are reports of cancer-associated IBM. We review the pathogenic mechanisms supposed to be involved in IBM and outline the common mechanisms in IBM and malignancy, as well as the therapeutic perspectives. The terminally differentiated, CD8+ highly cytotoxic T cells expressing NK features are central in the pathogenesis of IBM and, paradoxically, play a role in some cancers as well. Interferon gamma plays a central role, mostly during the early stages of the disease. The secondary mitochondrial dysfunction, the autophagy and cell cycle dysregulation, and the crosstalk between metabolic and mitogenic pathways could be shared by IBM and cancer. There are intermingled subcellular mechanisms in IBM and neoplasia, and probably their co-existence is underestimated. The link between IBM and cancers deserves further interest, in order to search for efficient therapies in IBM and to improve muscle function, life quality, and survival in both diseases.  相似文献   

    

Joanna Rydz  Khadar Duale  Henryk Janeczek  Wanda Sikorska  Andrzej Marcinkowski  Marta Musio&#x;  Marcin Godzierz  Aleksandra Kordyka  Micha&#x; Sobota  Cristian Peptu  Neli Koseva  Marek Kowalczuk 《International journal of molecular sciences》2022,23(14)

Poly(L-lactide) is capable of self-assembly into a nematic mesophase under the influence of temperature and mechanical stresses. Therefore, subsequent poly(L-lactide) films were obtained and characterized, showing nematic liquid crystal properties both before and after degradation. Herein, we present that, by introducing β-cyclodextrin into the polymer matrix, it is possible to obtain a chiral nematic mesophase during pressing, regardless of temperature and time. The obtained poly(L-lactide) films exhibiting liquid crystal properties were subjected to degradation tests and the influence of degradation on these properties was determined. Thermotropic phase behavior was investigated using polarized optical microscopy, X-ray diffraction, and differential scanning calorimetry. The degradation process demonstrated an influence on the liquid crystal properties of pressed polymer films. The colored planar texture of the chiral nematic mesophase, which was not observed prior to degradation in films without the addition of β-cyclodextrin, appeared after incubation in water as a result of the entrapment of degradation products in the polymer matrix. These unusual tailor-made properties, obtained in liquid crystals in (bio)degradable polymers using a simple method, demonstrate the potential for advanced photonic applications.  相似文献   

    

Cristina Stefania Dumitru  Amalia Raluca Ceausu  Nela Pusa Gaje  Cristian Silviu Suciu  Marius Raica 《International journal of molecular sciences》2022,23(17)

Podoplanin and Ki-67 are two important markers of cancer progression. The aim of this study is to evaluate double immunostaining for Ki-67 and podoplanin in head and neck squamous cell carcinoma (HNSCC), and to observe the involvement of lymphagiogenesis in tumoral and peritumoral areas, as well as the density of tumor proliferation correlated with histopathological grading. A total of 50 patients with HNSCC were included in this study. We carried out a morphological evaluation of tissue samples, after that, cases were selected for double Ki-67 and podoplanin immunostaining. Podoplanin expression was significantly correlated with histopathological grade (p < 0.05; p = 0.037) and expression of Ki-67 (p < 0.05; p = 0.050). A high expression of podoplanin, as well as of the proliferation factor Ki-67, was observed in histopathological grade G3 and the correlation between these (p < 0.05; p = 0.028), and implication of LMVD and LVI was not significant (LMVD p = 0.577; LVI p = 0.976). This study demonstrated the importance of double immunolabeling in assessing lymphagiogenesis and tumor proliferation in correlation with histopathological grades in HNSCC.  相似文献   

    

Cristian M. Pis Diez  Maria Juliana Juncos  Matias Villarruel Dujovne  Daiana A. Capdevila 《International journal of molecular sciences》2022,23(4)

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Carlos Lopez-Ortiz  Mary Edwards  Purushothaman Natarajan  Armando Pacheco-Valenciana  Padma Nimmakayala  Donald A. Adjeroh  Cristian Sirbu  Umesh K. Reddy 《International journal of molecular sciences》2022,23(17)

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Juan Manuel Velzquez-Enríquez  Alma Aurora Ramírez-Hernndez  Luis Manuel Snchez Navarro  Itayetzi Reyes-Avendao  Karina Gonzlez-García  Cristian Jimnez-Martínez  Luis Castro-Snchez  Xariss Miryam Snchez-Chino  Vernica Rocío Vsquez-Garzn  Rafael Baltirrez-Hoyos 《International journal of molecular sciences》2022,23(9)

Idiopathic pulmonary fibrosis (IPF) is a chronic, progressive, irreversible lung disorder of unknown cause. This disease is characterized by profibrotic activation of resident pulmonary fibroblasts resulting in aberrant deposition of extracellular matrix (ECM) proteins. However, although much is known about the pathophysiology of IPF, the cellular and molecular processes that occur and allow aberrant fibroblast activation remain an unmet need. To explore the differentially expressed proteins (DEPs) associated with aberrant activation of these fibroblasts, we used the IPF lung fibroblast cell lines LL97A (IPF-1) and LL29 (IPF-2), compared to the normal lung fibroblast cell line CCD19Lu (NL-1). Protein samples were quantified and identified using a label-free quantitative proteomic analysis approach by liquid chromatography-tandem mass spectrometry (LC-MS/MS). DEPs were identified after pairwise comparison, including all experimental groups. Gene Ontology (GO) enrichment analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG), and Protein–Protein Interaction (PPI) network construction were used to interpret the proteomic data. Eighty proteins expressed exclusively in the IPF-1 and IPF-2 clusters were identified. In addition, 19 proteins were identified up-regulated in IPF-1 and 10 in IPF-2; 10 proteins were down-regulated in IPF-1 and 2 in IPF-2 when compared to the NL-1 proteome. Using the search tool for retrieval of interacting genes/proteins (STRING) software, a PPI network was constructed between the DEPs and the 80 proteins expressed exclusively in the IPF-2 and IPF-1 clusters, containing 115 nodes and 136 edges. The 10 hub proteins present in the IPP network were identified using the CytoHubba plugin of the Cytoscape software. GO and KEGG pathway analyses showed that the hub proteins were mainly related to cell adhesion, integrin binding, and hematopoietic cell lineage. Our results provide relevant information on DEPs present in IPF lung fibroblast cell lines when compared to the normal lung fibroblast cell line that could play a key role during IPF pathogenesis.  相似文献   

    

Rune Matthiesen  Paula Gameiro  Andreia Henriques  Cristian Bodo  Maria Carolina Strano Moraes  Bruno Costa-Silva  Jos Cabeadas  Maria Gomes da Silva  Hans Christian Beck  Ana Sofia Carvalho 《International journal of molecular sciences》2022,23(21)

Diffuse large B cell lymphoma (DLBCL) is an aggressive B cell lymphoma characterized by a heterogeneous behavior and in need of more accurate biological characterization monitoring and prognostic tools. Extracellular vesicles are secreted by all cell types and are currently established to some extent as representatives of the cell of origin. The present study characterized and evaluated the diagnostic and prognostic potential of plasma extracellular vesicles (EVs) proteome in DLBCL by using state-of-the-art mass spectrometry. The EV proteome is strongly affected by DLBCL status, with multiple proteins uniquely identified in the plasma of DLBCL. A proof-of-concept classifier resulted in highly accurate classification with a sensitivity and specificity of 1 when tested on the holdout test data set. On the other hand, no proteins were identified to correlate with non-germinal center B-cell like (non-GCB) or GCB subtypes to a significant degree after correction for multiple testing. However, functional analysis suggested that antigen binding is regulated when comparing non-GCB and GCB. Survival analysis based on protein quantitative values and clinical parameters identified multiple EV proteins as significantly correlated to survival. In conclusion, the plasma extracellular vesicle proteome identifies DLBCL cancer patients from healthy donors and contains potential EV protein markers for prediction of survival.  相似文献   

    

Francesco Lorenzo Serafini  Andrea Delli Pizzi  Pasquale Simeone  Alberto Giammarino  Cristian Mannetta  Michela Villani  Jacopo Izzi  Davide Buca  Giulia Catitti  Piero Chiacchiaretta  Stefano Trebeschi  Sebastiano Miscia  Massimo Caulo  Paola Lanuti 《International journal of molecular sciences》2022,23(24)

Abdominal aortic aneurysm (AAA) is a frequent aortic disease. If the diameter of the aorta is larger than 5 cm, an open surgical repair (OSR) or an endovascular aortic repair (EVAR) are recommended. To prevent possible complications (i.e., endoleaks), EVAR-treated patients need to be monitored for 5 years following the intervention, using computed tomography angiography (CTA). However, this radiological method involves high radiation exposure in terms of CTA/year. In such a context, the study of peripheral-blood-circulating extracellular vesicles (pbcEVs) has great potential to identify biomarkers for EVAR complications. We analyzed several phenotypes of pbcEVs using polychromatic flow cytometry in 22 patients with AAA eligible for EVAR. From each enrolled patient, peripheral blood samples were collected at AAA diagnosis, and after 1, 6, and 12 months following EVAR implantation, i.e. during the diagnostic follow-up protocol. Patients developing an endoleak displayed a significant decrease in activated-platelet-derived EVs between the baseline condition and 6 months after EVAR intervention. Furthermore, we also observed, that 1 month after EVAR implantation, patients developing an endoleak showed higher concentrations of activated-endothelial-derived EVs than patients who did not develop one, suggesting their great potential as a noninvasive and specific biomarker for early identification of EVAR complications.  相似文献   

The multifunctional activity of water-soluble peptides’ extract of Domiati cheese during accelerated ripening by Neutrase     

Soad Taha  Moniur El Abd  Cristian De Gobba  Mahmoud Abdel-Hamid  Ensaf Khalil  Faiz-ul Hassan  Doaa Fathy 《Journal of Food Processing and Preservation》2020,44(5):e14434

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Magda I. Pinzon  Leidy T. Sanchez  Omar R. Garcia  Ramón Gutierrez  Julio C. Luna  Cristian C. Villa 《International Journal of Food Science & Technology》2020,55(1):92-98

Strawberries are among the most consumed fruits in the world, however, they are highly susceptible to both microbial and fungal contamination during storage. In this study, we used composite films made from banana starch-chitosan and Aloe vera gel (AV gel) at different AV gel concentrations. Our results show that AV gel inclusion can significantly reduce fungal decay, increasing strawberries shelf life up to 15 day of storage at the highest AV gel concentration (20%), while maintaining their physicochemical properties, such as colour and firmness. Weight loss was reduced 5% with respect of the uncoated fruit. Our results can be attributed to the limited water vapour transfer allowed by the edible coating, this is the result of the crosslinking effect of the different AV gel and starch molecules that allows a more controlled decay of the strawberries in commercial storage conditions.  相似文献