Response of key soil parameters during compost-assisted phytostabilization in extremely acidic tailings: effect of plant species

Phytostabilization of mine tailings acts to mitigate both eolian dispersion and water erosion events which can disseminate barren tailings over large distances. This technology uses plants to establish a vegetative cover to permanently immobilize contaminants in the rooting zone, often requiring addition of an amendment to assist plant growth. Here we report the results of a greenhouse study that evaluated the ability of six native plant species to grow in extremely acidic (pH ～ 2.5) metalliferous (As, Pb, Zn: 2000-3000 mg kg(-1)) mine tailings from Iron King Mine Humboldt Smelter Superfund site when amended with a range of compost concentrations. Results revealed that three of the six plant species tested (buffalo grass, mesquite, and catclaw acacia) are good candidates for phytostabilization at an optimum level of 15% compost (w/w) amendment showing good growth and minimal shoot accumulation of metal(loid)s. A fourth candidate, quailbush, also met all criteria except for exceeding the domestic animal toxicity limit for shoot accumulation of zinc. A key finding of this study was that the plant species that grew most successfully on these tailings significantly influenced key tailings parameters; direct correlations between plant biomass and both increased tailings pH and neutrophilic heterotrophic bacterial counts were observed. We also observed decreased iron oxidizer counts and decreased bioavailability of metal(loid)s mainly as a result of compost amendment. Taken together, these results suggest that the phytostabilization process reduced tailings toxicity as well as the potential for metal(loid) mobilization. This study provides practical information on plant and tailings characteristics that is critically needed for successful implementation of assisted phytostabilization on acidic, metalliferous mine tailings sites.     

Improved coverage of fungal diversity in polluted groundwaters by semi-nested PCR

Traditional methods used for studying communities of aquatic hyphomycetes are based on the detection and identification of their asexual spores under a microscope. These techniques limit detection to aquatic fungi present in sufficient quantity and capable of sporulating under laboratory conditions. Our objective was to develop a molecular approach to detect and monitor all types of fungi (i.e. strictly or facultatively aquatic) in harsh habitats (i.e. groundwater wells and heavily polluted surface water) where fungal biomass may become limited. We developed a semi-nested PCR protocol for fungal 18S ribosomal RNA genes coupled to subsequent analysis of the PCR products by Temperature Gradient Gel Electrophoresis (TGGE) to monitor the fungal community structure in aquatic habitats characterized by a pollution gradient. Our TGGE-protocol was compared with the traditional morphological approach and revealed a higher diversity in groundwaters and in some polluted surface waters. Thus, PCR-TGGE is a promising alternative in particular in habitats with low fungal biomass. The dynamics of fungal biomass and sporulation rates during the first weeks of leaf colonization showed that habitats with adverse ecological conditions allow only reduced fungal growth, which might subsequently impact upper trophic levels and thus interfere with key ecological processes of leaf decomposition.     

Cholinesterases activities and lipid peroxidation levels in muscle from shelf and slope dwelling fish from the NW Mediterranean: its potential use in pollution monitoring

We selected muscle tissue from nine commercial fish species, seven teleosts and two elasmobranchs, frequently caught in the NW Mediterranean trawling grounds in two different habitats (the continental shelf at 53 m and the slope at 660 m) for cholinesterases (ChEs) characterisation and activities determination, as well as the measure of lipid peroxidation (LP) levels. Acethyl- (AChE), butyryl- (BChE) and propionyl- (PrChE) cholinesterase were chosen as exposure markers of neurotoxic chemicals/molecules and LP as an effect marker of oxidative damage. The use of diagnose inhibitors: eserine sulphate, iso-OMPA and BW284c51 confirmed the measurement of true ChEs as well as the presence of pseudocholinesterases. The present study has evidenced the existence of interspecies differences, especially between teleosts and elasmobranchs but not in relation to depth. Moreover, the good correlation observed between all ChEs in most of the studied fish, supports the use of AChE as neurotoxic marker in field pollution monitoring. The non-metric multidimensional scaling analysis identified the commercial species hake Merluccius merluccius, among teleosts, and the shark, Galeus melastomus, as more adequate sentinel species in commercial fishing grounds. AChE activity showed a relationship with the fish trophic level, while all ChEs revealed a relationship with the feeding habits (benthic, suprabenthic, pelagic) and the stomach fullness. BChE activity and LP levels were the only markers to show a relationship with their swimming capacity.     

Heavy metal bioaccumulation and macroinvertebrate community changes in a Mediterranean stream affected by acid mine drainage and an accidental spill (Guadiamar River, SW Spain)

In the 1998 Aznalcóllar mining accident, 5 million cubic meters of toxic waste were spilled into the Guadiamar River (SW Spain). This paper describes the likely effects of metal pollution on the macroinvertebrate community in the first 15 km of the river adjacent to the mine, 2 years after the spill. The contents of Zn, Cu, Pb, As, Cd, Tl, and Sb in water, sediment, and in the caddisfly Hydropsyche were analysed, together with some physico-chemical parameters. From the results of physico-chemical and community parameters, the studied area was divided into three sections: the first section contained upstream control stations, not affected by current mining activities, and with a diverse macroinvertebrate community structure (mean number of families = 19); the second section, close to the mine, was severely polluted, with more than 50 mg l(-1) of Zn or 1.6 mg l(-1) of Cu in water, a pH of nearly 4, and a very diminished macroinvertebrate community (three families); in the last section, 6-15 km downstream from the mine, the water quality improved, metal concentrations decreased, pH reached neutrality and the macroinvertebrate community comprised eight families. We also determined metal concentrations in the larvae of Hydropsyche and found increases of 3- to 35-fold (for Cu and Cd, respectively) compared to control sites. As far as we are aware, these values are the highest concentrations so far detected in this caddisfly anywhere in the world. Different patterns of metal and pH tolerance were identified in macroinvertebrates. Most of the species were intolerant to present pollution levels and were only present in control sites. Others were tolerant to metal pollution but not to low pH and were present in the main river downstream of the mine, while a very few species managed to live in the most polluted section, with very low pH and high metal concentrations.     

Weight distribution and dual distance

Several results in coding theory (e.g. the Carlitz-Uchiyama bound) show that the weight distributions of certain algebraic codes of lengthn are concentrated aroundn/2 within a range of width n. It is proved in this article that the extreme weights of a linear binary code of sufficiently high dual distance cannot be too close ton/2, the gap being of order n. The tools used involve the Pless identities and the orthogonality properties of Krawtchouk polynomials, as well as estimates on their zeroes. As a by-product upper bounds on the minimum distance of self-dual binary codes are derived.     

Detection and Localization of Delaminations in Thin Carbon Fiber Reinforced Composites with the Ultrasonic Polar Scan

In this paper, the hybrid compliance-stiffness matrix method for simulating wave propagation in (delaminated) multilayered media with viscoelastic anisotropy has been confronted with high-quality amplitude and phase experiments on delaminated composites, obtained using the ultrasonic polar scan setup (UPS) in transmission by considering harmonic as well as pulsed ultrasound. Results are presented for multiple thin carbon/epoxy laminates with an artificial edge delamination induced by a foil insert, showing a good agreement between experimental recording and numerical modeling. The obtained results further reveal the feasibility of the harmonic UPS to detect and even locate the depth-position of multiple delaminations in fiber reinforced composites. Considering that the harmonic UPS method does not rely on the detection of different echoes like the classical C-scan, but rather expounds the conditions for efficient stimulation of guided waves in the solid, the method is found to be highly suited for inspecting thin composite materials for the presence of delaminations.     

Early anisotropic expansion of aluminium foam precursors

This work reports a detailed study on the early expansion (before the melting point) of powder metallurgical (PM) aluminium foam precursors and its influence on the intrinsic anisotropy existing in the final cellular structure of PM foams. Hot uniaxial compressed tablets, rectangular and cylindrical extruded profiles and thixocast PM precursors have been considered to evaluate the effect of the processing technique on the early expansion behaviour. An optical device has been used to register, in situ, the dissimilar expansion of the precursors in the three spatial directions. Cellular morphology has been examined by X-ray microtomography and correlated to expansion behaviour. Results demonstrated a high influence of the processing technique and its correlation with powder debonding in preferential directions which, in combination with the early TiH₂ decomposition, generates elongated cracks and causes an anisotropic expansion at early foaming stages. As a consequence, a remaining structural anisotropy is found in the final solid cellular material, even at high porosities. A discussion of the possible factors affecting this early expansion behaviour, complemented with possible solutions to minimize it, is provided in the paper.