Colorimetrische Bestimmung von Nitrat und Nitrit in biologischem Material

Zusammenfassung Die ausgearbeitete Methode zur Nitrat- und Nitritbestimmung beruht auf der colorimetrischen Bestimmung des durch Nitrierung entstehenden 6-Nitro-2,4-xylenols.Die Nitrite und Nitrate werden nebeneinander durch zwei Messungen bestimmt. Bei der ersten Messung werden Nitrite zu Nitraten oxydiert, bei der zweiten werden sie nach Überführung zu Methylnitrit entfernt. Die Differenz der ermittelten Werte entspricht den anwesenden Nitriten.Diese Methode eignet sich für Nitrat- und Nitritbestimmung im biologischen Material.     

Recent Progress in Neural Probes Using Silicon MEMS Technology

Extracellular recordings from the brain are the basis for the fundamental understanding of the complex interaction of electrical signals in neural information transfer. Going beyond wire electrodes and bundles of electrode wires such as tetrodes, multielectrode arrays based on silicon technologies are receiving growing attention, since they enable a pronounced increase in the number of recording sites per probe shaft. In this paper, recent innovations contributed by the authors to the development of probe arrays based on microelectromechanical system (MEMS) technologies within the EU‐funded research project NeuroProbes are described. The resulting structures include passive electrode arrays based on single‐shaft and four‐shaft probes comprising nine planar electrodes per shaft with lengths of up to 8 mm. Further, active probe arrays with complementary metal–oxide–semiconductor (CMOS) circuitry integrated on the probe shaft, enabling the arrangement of 188 electrodes in two columns along a 4‐mm‐long probe shaft with an electrode pitch of only 40 µm, are described. These active probes were developed for an electronic depth control. Further, the paper reports assembly technologies for combining the probe arrays with highly flexible ribbon cables. Applications of the probes in in vivo experiments are summarized. © 2010 Institute of Electrical Engineers of Japan. Published by John Wiley & Sons, Inc.     

Effect of location in the canopy on the colour development of three apple cultivars during growth

BACKGROUND: Homogeneity in appearance is one of the quality aspects asked for in the supply chain. Decreasing the biological variation in batches of harvested apples (cultivars Braeburn, Fuji and Gala) becomes increasingly important. Skin colour is one of the aspects that determine both optimal harvest and stage of development. Skin colour is affected by location in the canopy. The rules of development of biological variation are now established and will be used on skin colour data. RESULTS: The Minolta colour aspects a*, b* and L* measured before commercial harvest change in a sigmoidal fashion and can be analysed including the biological variation, with a logistic model in indexed nonlinear regression, obtaining explained parts of above 90%. The mechanism of colour change is not affected by state of development or location in the canopy. The location in the canopy affects the intensity of both red and green colouring compounds. The variation in colouration is not affected by the location in the canopy. CONCLUSION: The red‐coloured apple cultivar (Gala) depends more on the location in the canopy than the less‐coloured cultivars (Fuji and Braeburn). The colour development in Fuji apples is considerably slower, with a much larger variation in stage of development. The location in the canopy affects all aspects of biological variation (biological shift factor and asymptotic starting level of colouration) for all three colour aspects L*, a* and b*, but only the mean value, not the standard deviation. The biological shift factors per colour aspects are linearly related. Once induced, variation remains constant during development. Copyright © 2012 Society of Chemical Industry     

Comparison of a district heating system operation based on actual data – Omsk city,Russia, case study*

A district heating (DH) system in the Russian city of Omsk has been considered as a case study. This study is based on the data set of temperature and demand recorded over two-year operation of the DH system. We provide an insight on how the heat demand profile is met by the control framework. The paper quantifies the DH system performance by using heat load curves, maximum, minimum and average values of temperature, paving the way for a modelling approach. Central heating process in Omsk in 2017 was generally smoother than in 2016, while peak values are also lower. In 2017, its supply temperature was about 5°C closer to the control curve. The dispersion of the points was reduced between 2016 and 2017, especially at higher outdoor temperatures. Further lowering annual and peak heat demand, which are respectively 4% and 9% lower in 2017 should be a priority in future.     

Long-term stability of sodium borohydrides for hydrogen generation

As a source of the high purity hydrogen, sodium and potassium borohydrides are investigated in terms of long-term stability in the form of the concentrated solutions, heterogeneous mixtures and in the solid state corresponding to NaBH₄ or KBH₄ crystal hydrates. In order to improve their stability during the long-term storage sodium and potassium hydroxides were added to the initial borohydride compositions. The effect of temperature, concentration of the borohydride and the alkaline solution, and the nature of the cation in the alkaline solution on the rate of borohydride hydrolysis was investigated. The differential technique developed for evaluation of the rate of borohydride hydrolysis was successfully applied for the determination of the long-term stability of the water-alkaline solutions containing NaBH₄(KBH₄)·5H₂O with 1–10 wt.% of NaOH or KOH at 30 °C and 50 °C.     

Hydrogen gas sensing properties of WO3 sputter-deposited thin films enhanced by on-top deposited CuO nanoclusters

Magnetron-based gas aggregation cluster source (GAS) was used to prepare high-purity CuO (cupric oxide) nanoclusters on top of sputter-deposited thin film of tungsten trioxide (WO₃). The material was assembled as a conductometric hydrogen gas sensor and its response was tested and evaluated. It is demonstrated that addition of CuO clusters noticeably enhances the sensitivity of the pure WO₃ thin film. With an increasing amount of CuO clusters the sensitivity of CuO/WO₃ system rises further. When CuO clusters form a sufficiently thick and compact layer, the resistance response is reversed. Based on the sensorial behavior, conventional and near-ambient pressure X-Ray photoemission spectroscopies, and resistivity measurements, we propose that the sensing mechanism is based on the formation of nano-sized p-n junctions in between p-type CuO and n-type WO₃. The advantages of the GAS technique for preparing sensorial and/or catalytically active materials are emphasized.     

Systematic Analysis of the Improvements in Magnetic Resonance Microscopy with Ferroelectric Composite Ceramics

The spatial resolution and signal‐to‐noise ratio (SNR) attainable in magnetic resonance microscopy (MRM) are limited by intrinsic probe losses and probe–sample interactions. In this work, the possibility to exceed the SNR of a standard solenoid coil by more than a factor‐of‐two is demonstrated theoretically and experimentally. This improvement is achieved by exciting the first transverse electric mode of a low‐loss ceramic resonator instead of using the quasi‐static field of the metal‐wire solenoid coil. Based on theoretical considerations, a new probe for microscopy at 17 T is developed as a dielectric ring resonator made of ferroelectric/dielectric low‐loss composite ceramics precisely tunable via temperature control. Besides the twofold increase in SNR, compared with the solenoid probe, the proposed ceramic probe does not cause static‐field inhomogeneity and related image distortion.