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981.
König GM Kehraus S Seibert SF Abdel-Lateff A Müller D 《Chembiochem : a European journal of chemical biology》2006,7(2):229-238
The marine environment is distinguished by unique groups of organisms being the source of a wide array of fascinating structures. The enormous biodiversity of marine habitats is mirrored by the molecular diversity of secondary metabolites found in marine animals, plants and microbes. The recognition that many marine invertebrates contain endo- and epibiotic microorganisms and that some invertebrate-derived natural products are structurally related to bacterial metabolites suggests a microbial origin for some of these compounds. Other marine natural products, however, are clearly located in invertebrate tissue and microbial involvement in the biosynthetic process seems unlikely. The complexity of associations in marine organisms, especially in sponges, bryozoans and tunicates, makes it extremely difficult to definitively state the biosynthetic source of many marine natural products or to deduce their ecological significance. Whereas many symbiotic marine microorganisms cannot be isolated and cultured, numerous epi- and endobiotic marine fungi produce novel secondary metabolites in laboratory cultures. The potent biological activity of many marine natural products is of relevance for their ecological function but is also the basis of their biomedical importance. 相似文献
982.
Agafonov DE Rabe KS Grote M Voertler CS Sprinzl M 《Chembiochem : a European journal of chemical biology》2006,7(2):330-336
Deactivation of release factor 1 by polyclonal antibodies in an in vitro translation system, which was used to express the esterase gene, led to the reversible elimination of naturally occurring termination. This technique allowed the antibiotic puromycin to be used as an acceptor substrate for the peptidyl residue in the peptidyl-transferase reaction. This resulted in more than 80 % yield of protein with C-terminally incorporated puromycin. pCpPuromycin that was either conjugated with the Cy3 fluorophor or biotin by N4 alkylation of cytosine, also acted as an acceptor substrate for the peptidyl-transferase reaction and was incorporated into the protein C terminus. The resulting conjugates possessed Cy3-specific fluorescence and affinity to streptavidin-coated surfaces, respectively. This left the enzymatic activity of the reporter protein unaffected. It was also shown that extension of puromycin on its 5'-hydroxyl end by up to ten deoxyoligonucleotides also allowed conjugation with the C terminus of in vitro translated protein when RF1-dependent termination was suppressed. However, the conjugation yield decreased upon addition of more than six nucleotides. 相似文献
983.
Müller I Weinig S Steinmetz H Kunze B Veluthoor S Mahmud T Müller R 《Chembiochem : a European journal of chemical biology》2006,7(8):1197-1205
Secondary metabolism involves a broad diversity of biochemical reactions that result in a wide variety of biologically active compounds. Terminal amide formation during the biosynthesis of the myxobacterial electron-transport inhibitor, myxothiazol, was analyzed by heterologous expression of the unique nonribosomal-peptide synthetase, MtaG, and incubation with a synthesized substrate mimic. These experiments provide evidence that the terminal amide is formed from a carrier protein-bound myxothiazol acid that is thioesterified to MtaF. This intermediate is transformed to an amide by extension with glycine and subsequent oxidative cleavage by MtaG. The final steps of melithiazol assembly involve a highly similar protein-bound intermediate (attached to MelF, a homologue of MtaF), which is transformed to an amide by MelG (homologue of MtaG). In this study, we also show that the amide moiety of myxothiazol A can be hydrolyzed in vivo to the formerly unknown free myxothiazol acid by heterologous expression of melJ in the myxothiazol producer Stigmatella aurantiaca DW4/3-1. The methyltransferase MelK can finally methylate the acid to give rise to the methyl ester, which is produced as the final product in the melithiazol A biosynthetic pathway. These experiments clarify the role of MelJ and MelK during melithiazol assembly. 相似文献
984.
The synthesis of amphiphilic polyaza crown ether monomers X (benzyl-substituted), Y (palmityl-substituted) and Z (cholesteryl-substituted) and their incorporation into oligonucleotides are described. Their effects on thermal duplex stability were investigated by UV melting curve analysis in different alkaline metal buffer solutions. Thermal-denaturation experiments showed remarkable stabilization of dsDNA by polyaza crown ether monomers when incorporated in opposite positions. The series of polyaza crown ether monomers (X, Y and Z) with different overall lipophilicities showed a trend of increased stability of the corresponding dsDNA with increasing lipophilicity of the polyaza crown ether monomer. Multiple incorporations of benzyl-substituted polyaza crown ether monomer X as dangling ends on both sides of dsDNA resulted in strongly increased stability of the corresponding duplex. 相似文献
985.
Hydrogen energy from coupled waste gasification and cement production—a thermochemical concept study
A plant concept for hydrogen production from waste gasification coupled with cement manufacturing is presented. Hot precalcined cement meal, from the operating cement process, is used as heat carrier to provide energy required by the parallel arranged gasifier. The amount of CaO present in the cement meal operates simultaneously as an effective in situ CO2-sorbent. First, a practical case study was devised to be able to perform simulations for estimation of expected hydrogen yield. The influence of different operation parameters of the gasifier and the hydrogen separation unit (steam-to-fuel ratio, pyrolysis temperature, PSA efficiency) was studied based on chemical equilibrium calculations. The simulation results indicate, that the coupling provides advantages for both processes. The production of a hydrogen-rich gas via thermal gasification benefits from the continuously available fresh CaO, which improves fuel conversion reactions and captures CO2 in situ. High-calorific streams from gasification process remaining after hydrogen separation may substitute fossil fuels needed for cement process. For a steam/fuel ratio of 0.3 and a PSA efficiency of 0.7, the calculated hydrogen energy yield is 46% of fuel energy input. 相似文献
986.
Bonzini S Verro R Otto S Lazzaro L Finizio A Zanin G Vighi M 《Environmental science & technology》2006,40(24):7561-7569
A GIS-based procedure for predicting pesticide exposure in surface waters has been applied on a pilot river basin characterized by intensive agricultural activity. The predictive approach has been validated through experimental monitoring, performed by collecting manual and automatic water samples during the productive season. Five active ingredients (terbuthylazine, metolachlor, alachlor, linuron, fenitrothion) were selected for analysis to validate the predictive approach. Comparison between predicted and experimental values showed good agreement for terbuthylazine and metolachlor (used in large volumes within the basin), demonstrating the reliability of the approach. However, some anomalous results were obtained for some of the other chemicals, which serve to highlight the difficulties in getting reliable input data, in particular on application patterns (rate and time). Furthermore, the value of mapping pesticide exposure on the medium-large scale is described, and the limitations of the reported predictive approach are discussed. 相似文献
987.
The hyphenation of gas chromatography and mass spectrometry (GC/MS) revolutionized organic analysis. In GC/MS coupling, usually electron impact ionization is applied, and molecules are identified by their fragment pattern. Although mass spectrometry in principle is a separation method, it is used predominantly as a spectrometric technique. However, if soft (i.e., fragmentation-free) ionization techniques are applied, the inherent separation character of MS is emphasized, which has similarities to a GC boiling point separation. By combining polar column GC separation and fast soft ionization time-of-flight mass spectrometry technology, a comprehensive separation of complex petrochemical samples can be obtained (GC x MS approach). Compounds of comparable physical-chemical properties are characteristically grouped together in a two-dimensional retention time-m/z representation. This resembles the separation characteristics of comprehensive two-dimensional gas chromatography (GC x GC) and, thus, represents a novel multidimensional separation approach. In this work, a gas chromatograph equipped with a polar separation column was coupled to a home-built laser ionization time-of-flight mass spectrometer. Laser-based, single-photon ionization was used for universal soft ionization and resonance-enhanced multiphoton ionization for selective ionization of aromatic compounds. A novel capillary-jet inlet system was used for the coupling. Multidimensional comprehensive analysis of complex petrochemical hydrocarbon samples using gas chromatography coupled to mass spectrometry with soft and selective photo ionization sources is first demonstrated. 相似文献
988.
Chapman HN Barty A Marchesini S Noy A Hau-Riege SP Cui C Howells MR Rosen R He H Spence JC Weierstall U Beetz T Jacobsen C Shapiro D 《Journal of the Optical Society of America. A, Optics, image science, and vision》2006,23(5):1179-1200
Coherent x-ray diffraction microscopy is a method of imaging nonperiodic isolated objects at resolutions limited, in principle, by only the wavelength and largest scattering angles recorded. We demonstrate x-ray diffraction imaging with high resolution in all three dimensions, as determined by a quantitative analysis of the reconstructed volume images. These images are retrieved from the three-dimensional diffraction data using no a priori knowledge about the shape or composition of the object, which has never before been demonstrated on a nonperiodic object. We also construct two-dimensional images of thick objects with greatly increased depth of focus (without loss of transverse spatial resolution). These methods can be used to image biological and materials science samples at high resolution with x-ray undulator radiation and establishes the techniques to be used in atomic-resolution ultrafast imaging at x-ray free-electron laser sources. 相似文献
989.
Erik V. Johnson Sjoerd Hoogland Ethan Klem Nazir Kherani Stefan Zukotynski 《Journal of Materials Science: Materials in Electronics》2006,17(10):789-799
Characterization results on hydrogenated microcrystalline silicon (μc-Si:H) thin films grown in a Saddle Field (SF) PECVD
system are presented. The microcrystalline content of the films is controlled by the application of a positive electrical
bias to the film growth surface. The results of photoluminescence, atomic force microscopy, infrared-absorption, and electrical
conductivity studies are presented. The results correlate to the changing microcrystalline content of the films in the same
way as when microcrystalline content is influenced through growth parameters such as hydrogen dilution in other CVD techniques.
相似文献
Stefan ZukotynskiEmail: |
990.
We have recently described a revised scheme for spermatogonial expansion in non-human primates. We proposed that A(pale)-spermatogonia act as self-renewing progenitors and premeiotic germ cells are organized and divide as small clones. Here, we are revisiting the model described for man and propose a modified scheme for spermatogonial expansion. Our revised model shows high similarity to the scheme proposed for non-human primates and is in accordance with all previous and present data. 相似文献