技术员的“草根”狂想

老婆孩子远行两日，闲在家没什么事，出去踢了一场球，感觉还能跑，运动生命还没有结束。     

Stiffness analysis of tibia-implant system under cyclic loading

A testing method has been developed to characterize stiffness and permanent deformation of bone-implant systems. The system consists of an artificial tibia with simulated distal fracture stabilized by an unreamed intramedullary nail. This system was loaded with three different sequences at 2 Hz, each consisting of 40,000 sinusoidal cycles, simulating clinical relevant loading conditions. Evaluation of the results showed a stiffness of 2782 N/mm with a standard deviation of 311 N/mm and a permanent deformation of 0.64 mm with a standard deviation of 0.21 mm. The locking screws broke exclusively during the third loading sequence starting with the most proximal of the distal screws. The study provides a standard technique for biomechanical testing and a comparison of different bone/implant-systems avoiding the variability of cadaver bone tests.     

From the ganglioside GQ1balpha to glycomimetic antagonists of the myelin-associated glycoprotein (MAG)

The tetrasaccharide 4, a substructure of ganglioside GQ1balpha, shows a remarkable affinity for the myelin-associated glycoprotein (MAG) and was therefore selected as starting point for a lead optimization program. In our search for structurally simplified and pharmacokinetically improved mimics of 4, antagonists with modifications of the core disaccharide Galbeta(1-3)GalNAc, as well as the terminal alpha(2-3)- and the internal alpha(2-6)-linked neuraminic acid were synthesized and tested in target-based binding assays. Compared to the reference tetrasaccharide 4, the most potent antagonist 17 exhibits a 360-fold improved affinity. Furthermore, pharmacokinetic parameters such as stability in the cerebrospinal fluid, logD and permeation through the BBB indicate the drug-like properties of antagonist 17.     

Nanocomposite Microcontainers with High Ultrasound Sensitivity

A water suspension of nanocomposite microcapsules with embedded ZnO nanoparticles in the capsule shell is reported. The microcapsule morphology is characterized by confocal microscopy, TEM, SEM, and AFM before and after ultrasound treatment. A remarkably high capsule sensitivity to ultrasound is evidenced, and it is observed to grow with increasing number of ZnO nanoparticle layers in the nanocomposite shell. This effect is correlated with the mechanical properties of microcapsules measured with AFM.     

Comparison of plastic films with different optical properties for soil covering in horticulture: test under simulated environmental conditions

Covering the soil with plastic film is an important method to modify soil temperature and improve the conditions for plant growth. For the optimal use of films in horticulture, it is necessary to quantify this effect, which depends particularly on the optical properties of the film. Five films, used mainly for soil covering in asparagus cultivation, were tested by measuring the soil temperature as well as the transmittance and reflectance in the short‐ and long‐wavelength bands: ‘Taschenfolie schwarz/weiß’, a film with black and white surfaces and pockets for fastening; ‘Antitaufolie’, a film with anti‐condensation coating, ‘Solartherm plus Folie’, a film with black top and transparent sides; ‘MaterBi Folie’, a biodegradable film; and ‘Thermoplus Folie’, a black polyethylene with transparent sides. All films were produced in Germany. The temperature measurements were carried out under controlled irradiation (short‐wavelength band) and ambient temperature conditions in the laboratory: first, 182 W m^?2 and 11 °C at a depth of 0.4 m, and second, 340 W m^?2 at 20 °C. A significant relationship between the temperature increase or decrease by covering the soil with the film and the calculated apparent quantities of the reflectance in the short and long‐wavelength band was derived (r² = 0.85, n = 12). Furthermore, first tests with a physical model were carried out to obtain basic information, which should be used in the development of strategies for film management in the field. Copyright © 2004 Society of Chemical Industry     

A reduced integration solid‐shell finite element based on the EAS and the ANS concept—Large deformation problems

In this paper we address the extension of a recently proposed reduced integration eight‐node solid‐shell finite element to large deformations. The element requires only one integration point within the shell plane and at least two integration points over the thickness. The possibility to choose arbitrarily many Gauss points over the shell thickness enables a realistic and efficient modeling of the non‐linear material behavior. Only one enhanced degree‐of‐freedom is needed to avoid volumetric and Poisson thickness locking. One key point of the formulation is the Taylor expansion of the inverse Jacobian matrix with respect to the element center leading to a very accurate modeling of arbitrary element shapes. The transverse shear and curvature thickness locking are cured by means of the assumed natural strain concept. Further crucial points are the Taylor expansion of the compatible cartesian strain with respect to the center of the element as well as the Taylor expansion of the second Piola–Kirchhoff stress tensor with respect to the normal through the center of the element. Copyright © 2010 John Wiley & Sons, Ltd.     

Erythropoietin Modulates Autophagy Signaling in the Developing Rat Brain in an In Vivo Model of Oxygen-Toxicity

Autophagy is a self-degradative process that involves turnover and recycling of cytoplasmic components in healthy and diseased tissue. Autophagy has been shown to be protective at the early stages of programmed cell death but it can also promote apoptosis under certain conditions. Earlier we demonstrated that oxygen contributes to the pathogenesis of neonatal brain damage, which can be ameliorated by intervention with recombinant human erythropoietin (rhEpo). Extrinsic- and intrinsic apoptotic pathways are involved in oxygen induced neurotoxicity but the role of autophagy in this model is unclear. We analyzed the expression of autophagy activity markers in the immature rodent brain after exposure to elevated oxygen concentrations. We observed a hyperoxia-exposure dependent regulation of autophagy-related gene (Atg) proteins Atg3, 5, 12, Beclin-1, microtubule-associated protein 1 light chain 3 (LC3), LC3A-II, and LC3B-II which are all key autophagy activity proteins. Interestingly, a single injection with rhEpo at the onset of hyperoxia counteracted these oxygen-mediated effects. Our results indicate that rhEpo generates its protective effect by modifying the key autophagy activity proteins.