Characteristics of the heterologously expressed human lanosterol 14α‐demethylase (other names: P45014DM,CYP51, P45051) and inhibition of the purified human and Candida albicans CYP51 with azole antifungal agents

Human and Candida albicans CYP51 were purified to homogeneity after GAL10‐based heterologous expression in yeast in order to resolve the basis for the selective inhibition of the fungal enzyme over the human orthologue by the azole drugs ketoconazole and itraconazole, used in the treatment of systemic fungal infection. The purified proteins have similar spectral characteristics, both giving a maximum at 448 nm in reduced carbon monoxide difference spectra. Substrate affinity constants of 20·8 and 29·4 μM and V_max of 0·15 and 0·47 nmol/min/nmol were observed for C. albicans and human enzymes, respectively, in reconstituted enzymatic assays, using an intermediate of the demethylation reaction [32‐³H]‐3β‐hydroxylanost‐7‐en‐32‐ol as the substrate. Both enzymes gave similar type II spectra on titration with drugs, but a reduced affinity was observed for human CYP51 using the ability of carbon monoxide to displace the drug as a ligand and by calculation of IC₅₀. However, although the results indicate higher affinity of the drugs for their target CYP51 in the major fungal pathogen C. albicans, when compared directly to CYP51 from humans, the difference was less than 10‐fold. This difference is an order of magnitude lower than previously reported data based on measurements using unpurified human CYP51 enzyme preparations. Consequently, increased azole doses to combat resistant candidaemia may well inhibit endogenous human CYP51 and the potential consequences are discussed. Copyright © 1999 John Wiley & Sons, Ltd.     

基于高端FPGA的IC验证平台的电源完整性(PI)分析

通用的FPGA(Field Programmable Gate Array,现场可编程逻辑门阵列)验证平台在运行不同的应用时,其瞬态电流需求是不同的。本文以基于Xilinx公司的Virtex-4系列千万门级的FPGA高端IC验证平台为例,采用Cadence PCB PI(Power Integrity)分析软件,对多种地电平面、电容值、电容的放置位置、电容的类型等进行评估,然后通过修正电容数量和额定值,调整电容的布局以及封装等,达到符合要求的电源-地平面目标阻抗,从而将电源/地平面上的噪声降低到电源的要求范围内。本文提出的电源完整性分析方法,对其他类型的系统板级设计也有一定的指导意义。     

混合动力汽车锂离子电池监控——需要冗余吗?

目前,混合动力汽车(HEV)、插电式混合动力汽车(PHEV)和纯电动汽车(EV)的开发工作与锂离子(Li-ion)电池的使用密不可分,许多OEM、电子设备供应商和电池制造商探讨的重点都集中在如何构建最佳的电池监控和管理系统.这些系统当然需要一个主监控器,它通常是一个与微控制器或电池管理单元(BMU)接口的模数转换器(ADC),用来提供精确的测量数据并执行电池平衡(确保充电前所有电池处于同一电平).     

Detection of repolarization alternans with an implantable cardioverter defibrillator lead in a porcine model

Mechanistic links have been suggested between repolarization alternans (RPA) and the onset of ventricular tachycardia (VT) and/or fibrillation. Endocardial detection of RPA may, therefore, be an important step in future device-based treatments of arrhythmias. Here, we investigate if RPA could be detected during acute ischemia using an implantable cardioverter defibrillator (ICD) lead (tip to distal coil) located in the right ventricular apex. In 18 pigs, the right coronary (n = 10) or left anterior descending coronary (n = 8) artery was occluded for 10 min using a balloon catheter, followed by reperfusion for 30 min, and re-occlusion for 30 min. RPA magnitude, computed using the modified moving average (MMA) method, showed a sharp increase in all 18 animals, from a mean baseline level of 1.9 +/- 1.3 mV to 3.0 +/- 1.3 mV during first occlusion (p < 0.001). RPA magnitude showed a prominent increase in 10 animals during re-occlusion, from a mean baseline level of 1.7 +/- 1.0 mV to 3.3 +/- 1.5 mV (p < 0.001). The protocol was terminated during the first two stages of occlusion and reperfusion for the remaining 8 animals due to the occurrence of ventricular fibrillation (VF). These results confirm that RPA increases under ischemic conditions and that it is possible to detect and track RPA dynamics with an ICD lead that is positioned in a clinically realistic location. Such an approach may be useful in formulating improved arrhythmia detection and control algorithms.     

Recombination and trapping in multicrystalline silicon

Minority carrier recombination and trapping frequently coexist in multicrystalline silicon (mc-Si), with the latter effect obscuring both transient and steady-state measurements of the photoconductance. In this paper, the injection dependence of the measured lifetime is studied to gain insight into these physical mechanisms. A theoretical model for minority carrier trapping is shown to explain the anomalous dependence of the apparent lifetime with injection level and allow the evaluation of the density of trapping centers. The main causes for volume recombination in mc-Si, impurities and crystallographic defects, are separately investigated by means of cross-contamination and gettering experiments. Metallic impurities produce a dependence of the bulk minority carrier lifetime with injection level that follows the Shockley-Read-Hall recombination theory. Modeling of this dependence gives information on the fundamental electron and hole lifetimes, with the former typically being considerably smaller than the latter, in p-type silicon, Phosphorus gettering is used to remove most of the impurities and reveal the crystallographic limits on the lifetime, which can reach 600 μs for 1.5 Ωcm mc-Si. Measurements of the lifetime at very high injection levels show evidence of the Auger recombination mechanism in mc-Si. Finally, the surface recombination velocity of the interface between mc-Si and thermally grown SiO₂ is measured and found to be as low as 70 cm/s for 1.5 Ωcm material after a forming gas anneal and 40 cm/s after an anneal. These high bulk lifetimes and excellent surface passivation prove that mc-Si can have an electronic quality similar to that of single-crystalline silicon     

Charge‐Carrier Balance and Color Purity in Polyfluorene Polymer Blends for Blue Light‐Emitting Diodes

A study of an efficient blue light‐emitting diode based on a fluorescent aryl polyfluorene (aryl‐F8) homopolymer in an inverted device architecture is presented, with ZnO and MoO₃ as electron‐ and hole‐injecting electrodes, respectively. Charge‐carrier balance and color purity in these structures are achieved by incorporating poly(9,9‐dioctylfluorene‐co‐N‐(4‐butylphenyl)‐diphenylamine (TFB) into aryl‐F8. TFB is known to be a hole‐transporting material but it is found to act as a hole trap on mixing with aryl‐F8. Luminance efficiency of ≈6 cd A^?1 and external quantum efficiency (EQE) of 3.1% are obtained by adding a small amount (0.5% by weight) of TFB into aryl‐F8. Study of charge injection and transport in the single‐carrier devices shows that the addition of a small fraction of hole traps is necessary for charge‐carrier balance. Optical studies using UV–vis and fluorescence spectroscopic measurements, photoluminescence quantum yield, and fluorescence decay time measurements indicate that TFB does not affect the optical properties of the aryl‐F8, which is the emitting material in these devices. Luminance efficiency of up to ≈11 cd A^?1 and EQE values of 5.7% are achieved in these structures with the aid of improved out‐coupling using index‐matched hemispheres.     

Indium tin oxide-free semi-transparent inverted polymer solar cells using conducting polymer as both bottom and top electrodes

Poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS) is investigated as a transparent cathode to replace indium tin oxide (ITO) in inverted polymer solar cells. Increasing the thickness of the PEDOT:PSS electrode leads to a reduction in transparency and sheet resistance which lowers the photocurrent but increases the fill factor of the solar cells. The offset of photocurrent and fill factor as the thickness is increased leads to a saturation of the power conversion efficiency to 3%. These electrodes were applied to flexible substrates showing similar device performance to glass based devices. Cyclic bending test of these flexible polymer electrodes show improved conversion efficiency retention (92%) when compared to flexible ITO based electrodes (50%) after 300 bend cycles. In addition to using PEDOT:PSS as a cathode replacement for ITO in inverted solar cells, its use as a semi-transparent anode replacement to Ag is also examined. Semi-transparent inverted solar cells fabricated with ITO as the cathode and PEDOT:PSS as the top anode electrode were demonstrated showing efficiencies of 2.51% while replacement of both ITO and Ag with PEDOT:PSS as both the cathode and anode show efficiencies of 0.47%.     

Two-dimensional synthetic aperture images over a land surface scene

The Soil Moisture and Ocean Salinity (SMOS) space mission is currently undergoing phase-B studies at the European Space Agency. The SMOS payload is an L-band interferometric radiometer based on a two-dimensional aperture synthesis concept. This paper presents the first images obtained by a demonstrator of the SMOS instrument over land surfaces at the Avignon test site in 1999     

Modelling of electrostatic gate operations in the Kane solid state quantum computer

The nuclear spin quantum computer proposed by Kane [Nature 393 (1998) 133] exploits as a qubit array ³¹P dopants embedded within a silicon matrix. Single-qubit operations are controlled by the application of electrostatic potentials via a set of metallic ‘A’ gates, situated above the donors, on the silicon surface, that tune the resonance frequency of individual nuclear spins, and a globally applied RF magnetic field that flips spins at resonance. Coupling between qubits is controlled by the application of potentials via a set of ‘J’ gates, between the donors, that induce an electron-mediated coupling between nuclear spins. We report the results of the study of the electric field and potential profiles arising within the Kane device from typical gate operations. The extent to which a single nuclear spin can be tuned independently of its neighbours, by operation of an associated A-gate, is examined and key design parameters in the Kane architecture are addressed. Implications for current fabrication strategies involving the implantation of ³¹P atoms are discussed. Solution of the Poisson equation has been carried out by simulation using a TCAD modelling package (Integrated Systems Engineering AG).     

Near optimal routing and capacity management for PWCE-based survivable WDM networks

Protected Working Capacity Envelope (PWCE) has been proposed to simplify resource management and traffic control for survivable WDM networks. In a PWCE-based network, part of the link capacity is reserved for accommodating working routes, and the remaining capacity is reserved for backup routes. The shortest path routing is applied in PWCE-based networks. An arrival call is accepted only when each link along the shortest path has a free working channel. Such a working path routing scheme greatly simplifies the call admission control process for dynamic traffic, and it is especially suitable for implementation in a distributed manner among network nodes. In this article, we investigate two protection strategies: Bundle Protection (BP) and Individual Protection (IDP). In BP, only one backup path can be used to protect a failure component, whereas multiple backup paths can be used in IDP. We formulate four mixed integer non-linear programming (MINLP) problems using BP and IDP strategies for single link and single node failure protection. Each model is designed to determine link metrics for shortest working path routing, working and backup channel assignments, and backup path planning. Our objective is to minimize call-blocking probability on the bottleneck link. Since these models are highly non-linear and non-convex, it is difficult to obtain exact global optimal solutions. We propose a Simulated Annealing-based Heuristic (SAH) algorithm to obtain near optimal solutions. This SAH adopts the concepts of simulated annealing as well as the bi-section technique to minimize call-blocking probabilities. To evaluate the performance, we made simulation comparisons between SAH and the unity link weight assignment scheme. The results indicate that SAH can greatly reduce call-blocking probabilities on benchmark and the randomly generated networks.