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排序方式: 共有385条查询结果,搜索用时 15 毫秒
381.
Chunyan Wang Mark Howell Payal Raulji Yvonne Davis Subhra Mohapatra 《Advanced functional materials》2011,21(23):4423-4429
Natriuretic peptide receptor A (NPRA), the receptor for the cardiac hormone atrial natriuretic peptide (ANP), is expressed abundantly on cancer cells and disruption of ANP‐NPRA signaling inhibits tumor burden and metastasis. Since antagonists of NPRA signaling have not provided reproducible results, we reason that a synthetic neutralizing antibody to ANP, which has high selectivity and affinity for ANP, can be used to regulate ANP levels and attenuate NPRA signaling. In this study, we prepare molecularly imprinted polymeric nanoparticles (MIPNPs) for ANP using a short peptide of ANP as the template and determine their binding affinity and selectivity. The MIPNPs are prepared by precipitation polymerization using NH2–SLRRSS–CONH2, which is a short peptide from ANP, as a template, methacrylic acid and N‐isopropylacrylamide as functional monomers, and bis‐acrylamide as a crosslinker. The average diameters of the MIPNPs and of non‐imprinted nanoparticles (NIPNPs) in water are 215.8 ± 4.6 nm and 197.7 ± 3.1 nm respectively. The binding‐isotherm analysis shows that the MIPNPs have a much‐higher binding affinity for the template peptide and ANP than the NIPNPs. Scatchard analysis gives an equilibrium dissociation constant, Kd, of 7.3 × 10?6 M with a binding capacity of 106.7 μmol g?1 for the template peptide and a Kd of 7.9 × 10?6 M with a binding capacity of 36.0 μmol g?1 for the ANP. Measurements of the binding kinetics reveal that MIPNPs reach protein‐adsorption equilibrium in 30 min. The MIPNPs are found to have a high specificity for ANP with little affinity for BSA or scrambled ANP peptide. The MIPNPs also recognize and adsorb ANP in cell‐culture medium spiked with ANP and in human plasma. Taken together, these results indicate that the MIPNPs have a high affinity and selectivity for ANP and can be used as a synthetic antibody for modulating ANP‐NPRA signaling in cancers. 相似文献
382.
M. Mohapatra R.M. KadamR.K. Mishra D. DuttaP.K. Pujari C.P. KaushikR.J. Kshirsagar B.S. TomarS.V. Godbole 《Nuclear instruments & methods in physics research. Section B, Beam interactions with materials and atoms》2011,269(19):2057-2062
Spectroscopic investigations were carried out on electron beam irradiated sodium barium borosilicate glasses, which is the base glass for immobilization of nuclear high level radioactive waste, generated from the research reactors at Bhabha Atomic Research Centre, Trombay. This was done in order to access the defects generated in it under long term irradiation. Electron paramagnetic resonance was used to identify the defect centers generated in the borosilicate glass after irradiation. In addition, positron annihilation spectroscopy and infrared investigations were done on the samples to evaluate the radiation induced changes in the glass. It was found that, boron-oxygen and silicon based hole centers along with E′ centers are getting formed in the glass after irradiation due to the breaking of the SiO bonds at regular tetrahedron sites of SiOSi. The positron annihilation spectroscopy data gave an idea regarding the free volume size and fraction of the glasses before and after irradiation. It was seen that, after irradiation the free volume size in the glass increased with creation of additional sites. Microwave power variation and temperature variation studies suggested the formation of at least five different radicals in the irradiated glasses. The spin Hamiltonian parameter of all the radical species were determined by computer simulation. An electron paramagnetic resonance spin counting technique was employed to evaluate the defect concentration in the glasses after irradiation. 相似文献
383.
Poly(lactic acid) (PLA) nanocomposites were prepared by melt mixing technique in a Haake batch mixer. The clay dispersion within the PLA matrix during melt mixing was well explained through the morphological characterization. Morphological characterizations were studied by X‐ray diffraction and transmission electron microscopy. The exfoliation/intercalation of the clay particles within the polymer matrix during melt mixing depends on the mixing torque generated during the preparation of nanocomposites. The significance of processing temperature and the mixing time in melt mixing were studied for PLA/C93A and PLA/C30B nanocomposites. The structure and properties of the nanocomposites were also characterized by differential scanning calorimetry, thermogravimetric analysis, dynamic mechanical analysis, and mechanical properties by standard tensile testing. The incorporation of nanoclays into the PLA matrix enhanced the mechanical properties and thermal stability of the PLA nanocomposites. This may be due to the reinforcing effect of nanoclays within the polymer matrix. POLYM. COMPOS., 2012. © 2012 Society of Plastics Engineers 相似文献
384.
Indrajit D. Thorat Debabandya Mohapatra R. F. Sutar S. S. Kapdi Dipali D. Jagtap 《Food and Bioprocess Technology》2012,5(4):1379-1383
The vacuum-drying characteristics of ginger (Zingiber officinale R.) slices were investigated. Drying experiments were carried out at a constant chamber pressure of 8 kPa, and at four different
drying temperatures (40 °C, 50 °C, 60 °C, and 65 °C).The effects of drying temperature on the drying rate and moisture ratio
of the ginger samples were evaluated. Efficient model for describing the vacuum-drying process was chosen by fitting five
commonly used drying models and a suggested polynomial was fitted to the experimental data. The effective moisture diffusivity
and activation energy were calculated using an infinite series solution of Fick’s diffusion equation. The results showed that
increasing drying temperature accelerated the vacuum-drying process. All drying experiments had only falling rate period.
The goodness of fit tests indicated that the proposed two-term exponential model gave the best fit to experimental results
among the five tested drying models. The average effective diffusivity values varied from 1.859 × 10−8 to 4.777 × 10−8 m2/s over the temperature range. The temperature dependence of the effective moisture diffusivity for the vacuum drying of the
ginger samples was satisfactorily described by an Arrhenius-type relationship with activation energy value of 35.675 kJ/mol
within 40–65 °C temperature range. 相似文献
385.
Ashis Kumar Mishra Subasish Mohapatra Pradip Kumar Sahu 《Concurrency and Computation》2024,36(10):e8005
Resource management and job scheduling are essential in today's cloud computing world. Due to task scheduling and users' diverse submission of large-scale requests, co-located VM instances negatively impacted the performance of leased VM instances. This workload further led to resource rivalry across co-located VMs. In order to address the aforementioned problems, numerous strategies have been presented, however, they fail to take the asynchronous nature of the cloud environment into account. To address this issue, a novel “CTA using DLFC-NN model” is proposed. This proposed approach combines the coalition theory and DLFC-NN techniques by including IRT-OPTICS for task size clustering, digital metrology based on ionized information (DMBII) for defect detection in virtue machines (VM), and the dynamic levy flight hamster optimization algorithm for processing time optimization of the clusters. However, the implementation of task scheduling in an online environment is limited by a number of presumptions or oversimplifications made by current scheduling systems. As a result, a unique coalition theory is applied to efficiently schedule activities. In addition, the DLFC-NN model is used to reduce resource consumption, span time, and be highly accurate and energy-efficient when working on both online and offline jobs. Nevertheless, while optimizing the clusters' overall execution time, earlier approaches only decreased the make-span time for task scheduling. However, the DLFC-NN model solves the computation problem by using a fully weighted bipartite graph and the pseudo method to determine the fitness of the least makespan time. The enhanced methodology used in this study reduces the scheduling cost and minimizes job completion times according to different task counts when compared to the existing techniques. 相似文献