The covalent functionalization of graphene oxide (GO) with chitosan (CS) is successfully accomplished via a facile amidation process. The CS-grafted GO (GO-CS) sheets consist of about 64 wt.% CS, which imparts them with a good aqueous solubility and biocompatibility. Additionally, the physicochemical properties of GO-CS are studied. As a novel nanocarrier, GO-CS is applied to load a water-insoluble anticancer drug, camptothecin (CPT), via π-π stacking and hydrophobic interactions. It is demonstrated that GO-CS possesses a superior loading capacity for CPT, and the GO-CS-CPT complexes show remarkably high cytotoxicity in HepG2 and HeLa cell lines compared to the pure drug. At the same time, GO-CS is also able to condense plasmid DNA into stable, nanosized complexes, and the resulting GO-CS/pDNA nanoparticles exhibit reasonable transfection efficiency in HeLa cells at certain nitrogen/phosphate ratios. Therefore, the GO-CS nanocarrier is able to load and deliver both anticancer drugs and genes. 相似文献
Extensive use of electroencephalogram (EEG) signals in diversified fields has put in a lot of thrust in research for devices capable of operating at constrained power and storage levels. In this paper, a simple and novel method for compression of multichannel EEG (MCEEG) signal is proposed. Here, wave atom transform of MCEEG data followed by quantization, thresholding, and arithmetic coding of context adaptive residuals and threshold coefficients is performed to achieve compression with good signal quality. The proposed method has been tested on a wide range of publicly available databases and results indicate that the algorithm is able to achieve good signal compression without degrading the signal quality. The proposed system provides an average compression ratio of 14.01 with a percentage root mean square difference of 1.91% across different data sets.
Studies of the binding of heme/hemin to proteins or peptides have recently intensified as it became evident that heme serves not only as a prosthetic group, but also as a regulator and effector molecule interacting with transmembrane and cytoplasmic proteins. The iron‐ion‐containing heme group can associate with these proteins in different ways, with the amino acids Cys, His, and Tyr allowing individual modes of binding. Strong coordinate‐covalent binding, such as in cytochrome c, is known, and reversible attachment is also discussed. Ligands for both types of binding have been reported independently, though sometimes with different affinities for similar sequences. We applied a combinatorial approach using the library (X)4(C/H/Y)(X)4 to characterize peptide ligands with considerable hemin binding capacities. Some of the library‐selected peptides were comparable in terms of hemin association independently of whether or not a cysteine residue was present in the sequence. Indeed, a preference for His‐based (≈39 %) and Tyr‐based (≈40 %) sequences over Cys‐based ones (≈21 %) was detected. The binding affinities for the library‐selected peptides, as determined by UV/Vis spectroscopy, were in the nanomolar range. Moreover, selected representatives efficiently competed for hemin binding with the human BK channel hSlo1, which is known to be regulated by heme through binding to its heme‐binding domain. 相似文献
This paper describes the growth of barium chromate (BaCrO4) nanocrystallites within thermally evaporated thin films of stearic acid (StA) and sodium bis-2-ethylhexyl-sulfosuccinate by a process of Ba2+ ion entrapment followed by in situ reaction with CrO42− ions. Dense spherical assemblies of BaCrO4 nanocrystallites of very uniform size (∼50 nm) were obtained within the two different host matrices. The spherical assemblies were composed of smaller (ca. 5–10 nm size) BaCrO4 crystals indicating that efficient size control over crystal size may be exercised by the matrix. Contact angle measurements of the BaCrO4–StA and BaCrO4–sodium bis-2-ethylhexyl-sulfosuccinate films indicated that they were hydrophobic, thus pointing to the possible role of hydrophobic interaction between the StA and sodium bis-2-ethylhexyl-sulfosuccinate monolayer-covered BaCrO4 crystals in the assembly process. 相似文献
Seeded growth of gold nanorods (NRs) has been accomplished in a micellar medium containing mixed surfactants or a high salt
concentration. Cetyl trimethylamoniumbromide (CTAB) forms micelles upon which the growth of rod shaped gold nanoparticles
occurs. AgNO3 is introduced into the growth solution to enhance the formation of NRs. The roles of non-ionic surfactants such as Tween
and Triton, and of electrolytes such as sodium chloride and potassium chloride have been examined. As the concentration of
these additives in the growth solution is increased, the aspect ratio of the NRs increases to a critical limit, after which
it decreases again. Upon carefully controlling the content of Triton X-100 or Tween 20 in the growth solution, these non-ionic
surfactants assisted in fine-tuning the shape of gold NRs (e.g. rectangular or “dogbone”). The growth pattern of the NRs fits
into the model of a soft template formed by the mixture of CTAB and non-ionic surfactants. 相似文献
The objective of this study is to prepare lanthanum and cerium metals by fused salt electrolysis of their anhydrous chloride in molten media such as LiCl-KCl, NaCl-KCl, KCl, NaCl, and LiCl and to characterize the metal deposit by X-ray diffraction, energy dispersive X-ray fluorescence, and inductive coupled plasma-atomic emission spectroscopy. Deposit metal of purity more than 99 % was obtained in each of the experiments. The entire process starting from preparation of anhydrous lanthanum/cerium chloride to electrolysis yielding of metal deposits has been described. The effect of process parameters such as temperature, electrolyte composition, and current density on the current efficiency was studied. All these parameters were varied to get the highest current efficiency and metal yield. The major non-rare earth impurities with the deposit are found to be Fe, Cr, and Ni along with ~1×10-3 of total gaseous impurities. 相似文献
The flow and heat transfer characteristics in the cooling of a heated surface by impinging slot jets have been investigated numerically. Computations are done for vertically downward-directed two-dimensional slot jets impinging on a hot isothermal surface at the bottom and confined by a parallel adiabatic surface on top. Some computations are also performed where the jet is vertically upward, with an impingement plate at the top. The principal objective of this study is to investigate the associated heat transfer process in the mixed-convective regime. The computed flow patterns and isotherms for various domain aspect ratios (4–10) and for a range of jet exit Reynolds numbers (100–500) and Richardson numbers (0–10) are analyzed to understand the mixed-convection heat transfer phenomena. The local and average Nusselt numbers and skin friction coefficients at the hot surface for various conditions are presented. It is observed that for a given domain aspect ratio and Richardson number, the average Nusselt number at the hot surface increases with increasing jet exit Reynolds number. On the other hand, for a given aspect ratio and Reynolds number, the average Nusselt number does not change significantly with Richardson number, indicating that the buoyancy effects are not very significant in the overall heat transfer process for the range of jet Reynolds number considered in this study. Also, for the same problem configuration, the average Nusselt number does not change significantly when the jet is moving upward or downward. 相似文献
The paper deals with effect of Fe on the solidification behaviour and mechanical properties of unmodified and modified Al–V–Si alloys. Effect of thermo-mechanical processing on the mechanical properties of these alloys was also reported. The solidification proceeds through several invariant reactions, the first one corresponds to formation of Al3(Fe,V,Si)-type phase. Modification with Ni–Mg master alloy changes the morphology, size and distribution of the primary as well as interdendritic phases. The modified alloys show an increase in first invariant reaction temperature and decrease in final invariant reaction temperature when compared with unmodified alloy, probably due to action of phase modification. In comparison to untreatable alloy, appreciable improvement in mechanical properties occurs on modification by Ni–Mg treatment. Hot rolling further improves the mechanical properties of the alloy. 相似文献
We report molecular changes in leaves of rice plants (Oryza sativa L. - reference crop plant and grass model) exposed to ultra low-dose ionizing radiation, first using contaminated soil from the exclusion zone around Chernobyl reactor site. Results revealed induction of stress-related marker genes (Northern blot) and secondary metabolites (LC-MS/MS) in irradiated leaf segments over appropriate control. Second, employing the same in vitro model system, we replicated results of the first experiment using in-house fabricated sources of ultra low-dose gamma (γ) rays and selected marker genes by RT-PCR. Results suggest the usefulness of the rice model in studying ultra low-dose radiation response/s. 相似文献